Synthesis and characterization of nanometer-sized liposomes for encapsulation and microRNA transfer to breast cancer cells

Henry Lujan,1 Wezley C Griffin,2,3 Joseph H Taube,2,4 Christie M Sayes1,41Department of Environmental Science, Baylor University, Waco, TX, USA; 2Department of Biology, Baylor University, Waco, TX, USA; 3Department of Chemistry and Biochemistry, Baylor University, Waco, TX, USA; 4I...

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Autores principales:	Lujan H, Griffin WC, Taube JH, Sayes CM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Liposome Nanoliposome microRNA Nanomaterial drug product Physicochemical characterization Drug delivery Breast cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2b2dcffd1d1242e48c92614431991644
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spelling	oai:doaj.org-article:2b2dcffd1d1242e48c926144319916442021-12-02T02:50:31ZSynthesis and characterization of nanometer-sized liposomes for encapsulation and microRNA transfer to breast cancer cells1178-2013https://doaj.org/article/2b2dcffd1d1242e48c926144319916442019-07-01T00:00:00Zhttps://www.dovepress.com/synthesis-and-characterization-of-nanometer-sized-liposomes-for-encaps-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Henry Lujan,1 Wezley C Griffin,2,3 Joseph H Taube,2,4 Christie M Sayes1,41Department of Environmental Science, Baylor University, Waco, TX, USA; 2Department of Biology, Baylor University, Waco, TX, USA; 3Department of Chemistry and Biochemistry, Baylor University, Waco, TX, USA; 4Institute for Biomedical Sciences, Baylor University, Waco, TX, USAIntroduction: The use of liposomes as a drug delivery carrier (DDC) for the treatment of various diseases, especially cancer, is rapidly increasing, requiring more stringent synthesis, formulation, and preservation techniques to bolster safety and efficacy. Liposomes otherwise referred to as phospholipid vesicles are self-assembled colloidal particles. When formed in either the micrometer or nanometer size range, they are ideal candidates as DDC because of their biological availability, performance, activity, and compatibility. Defining and addressing the critical quality attributes (CQAs) along the pharmaceutical production scale will enable a higher level of quality control for reproducibility. More specifically, understanding the CQAs of nanoliposomes that dictate its homogeneity and stability has the potential to widen applications in biomedical science.Methods: To this end, we designed a study that aimed to define synthesis, characterization, formulation (encapsulation), preservation, and cargo delivery and trafficking as the major components within a target product profile for nanoliposomes. A series of synthetic schemes were employed to measure physicochemical properties relevant to nanomaterial drug product development, including concentration gradients, probe versus bath sonication, and storage temperature measured by microscopy (electron and light) and dynamic light scattering.Results: Concentration was found to be a vital CQA as reducing concentrations resulted in nanometer-sized liposomes of <350 nm. Liposomes were loaded with microRNA and fluorescence spectroscopy was used to determine loading efficacy and stability over time. Lyophilization was used to create a dry powder formulation that was then assessed for stability for 6 months. Lastly, breast cancer cell lines were used to ensure efficacy of microRNA delivery and localization.Conclusion: We conclude that microRNA can be loaded into nanometer-sized liposomes, preserved for months in a dried form, and maintain encapsulation after extended time periods in storage.Keywords: liposome, microRNA, nanomaterial drug product, physicochemical characterization, drug delivery, breast cancerLujan HGriffin WCTaube JHSayes CMDove Medical PressarticleLiposomeNanoliposomemicroRNANanomaterial drug productPhysicochemical characterizationDrug deliveryBreast cancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 5159-5173 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Liposome Nanoliposome microRNA Nanomaterial drug product Physicochemical characterization Drug delivery Breast cancer Medicine (General) R5-920
spellingShingle	Liposome Nanoliposome microRNA Nanomaterial drug product Physicochemical characterization Drug delivery Breast cancer Medicine (General) R5-920 Lujan H Griffin WC Taube JH Sayes CM Synthesis and characterization of nanometer-sized liposomes for encapsulation and microRNA transfer to breast cancer cells
description	Henry Lujan,1 Wezley C Griffin,2,3 Joseph H Taube,2,4 Christie M Sayes1,41Department of Environmental Science, Baylor University, Waco, TX, USA; 2Department of Biology, Baylor University, Waco, TX, USA; 3Department of Chemistry and Biochemistry, Baylor University, Waco, TX, USA; 4Institute for Biomedical Sciences, Baylor University, Waco, TX, USAIntroduction: The use of liposomes as a drug delivery carrier (DDC) for the treatment of various diseases, especially cancer, is rapidly increasing, requiring more stringent synthesis, formulation, and preservation techniques to bolster safety and efficacy. Liposomes otherwise referred to as phospholipid vesicles are self-assembled colloidal particles. When formed in either the micrometer or nanometer size range, they are ideal candidates as DDC because of their biological availability, performance, activity, and compatibility. Defining and addressing the critical quality attributes (CQAs) along the pharmaceutical production scale will enable a higher level of quality control for reproducibility. More specifically, understanding the CQAs of nanoliposomes that dictate its homogeneity and stability has the potential to widen applications in biomedical science.Methods: To this end, we designed a study that aimed to define synthesis, characterization, formulation (encapsulation), preservation, and cargo delivery and trafficking as the major components within a target product profile for nanoliposomes. A series of synthetic schemes were employed to measure physicochemical properties relevant to nanomaterial drug product development, including concentration gradients, probe versus bath sonication, and storage temperature measured by microscopy (electron and light) and dynamic light scattering.Results: Concentration was found to be a vital CQA as reducing concentrations resulted in nanometer-sized liposomes of <350 nm. Liposomes were loaded with microRNA and fluorescence spectroscopy was used to determine loading efficacy and stability over time. Lyophilization was used to create a dry powder formulation that was then assessed for stability for 6 months. Lastly, breast cancer cell lines were used to ensure efficacy of microRNA delivery and localization.Conclusion: We conclude that microRNA can be loaded into nanometer-sized liposomes, preserved for months in a dried form, and maintain encapsulation after extended time periods in storage.Keywords: liposome, microRNA, nanomaterial drug product, physicochemical characterization, drug delivery, breast cancer
format	article
author	Lujan H Griffin WC Taube JH Sayes CM
author_facet	Lujan H Griffin WC Taube JH Sayes CM
author_sort	Lujan H
title	Synthesis and characterization of nanometer-sized liposomes for encapsulation and microRNA transfer to breast cancer cells
title_short	Synthesis and characterization of nanometer-sized liposomes for encapsulation and microRNA transfer to breast cancer cells
title_full	Synthesis and characterization of nanometer-sized liposomes for encapsulation and microRNA transfer to breast cancer cells
title_fullStr	Synthesis and characterization of nanometer-sized liposomes for encapsulation and microRNA transfer to breast cancer cells
title_full_unstemmed	Synthesis and characterization of nanometer-sized liposomes for encapsulation and microRNA transfer to breast cancer cells
title_sort	synthesis and characterization of nanometer-sized liposomes for encapsulation and microrna transfer to breast cancer cells
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/2b2dcffd1d1242e48c92614431991644
work_keys_str_mv	AT lujanh synthesisandcharacterizationofnanometersizedliposomesforencapsulationandmicrornatransfertobreastcancercells AT griffinwc synthesisandcharacterizationofnanometersizedliposomesforencapsulationandmicrornatransfertobreastcancercells AT taubejh synthesisandcharacterizationofnanometersizedliposomesforencapsulationandmicrornatransfertobreastcancercells AT sayescm synthesisandcharacterizationofnanometersizedliposomesforencapsulationandmicrornatransfertobreastcancercells
_version_	1718402092121456640

Synthesis and characterization of nanometer-sized liposomes for encapsulation and microRNA transfer to breast cancer cells

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