Molecular Characterization of Blastocystis sp. in Camelus bactrianus in Northwestern China

Blastocystis sp. is an important zoonotic protist in humans and various animals with worldwide distribution. However, there have been no data on the occurrence of Blastocystis sp. in C. bactrianus, an important economic animal in northwestern...

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Autores principales:	Xin Yang, Yunhui Li, Yuxin Wang, Junwei Wang, Peng Lai, Yuan Li, Junke Song, Meng Qi, Guanghui Zhao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Blastocystis</i> sp. <i>Camelus bactrianus</i> prevalence subtyping northwestern China Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/2b31688561704dbf8dbf25641d6efa18
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spelling	oai:doaj.org-article:2b31688561704dbf8dbf25641d6efa182021-11-25T16:13:38ZMolecular Characterization of <i>Blastocystis</i> sp. in <i>Camelus bactrianus</i> in Northwestern China10.3390/ani111130162076-2615https://doaj.org/article/2b31688561704dbf8dbf25641d6efa182021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3016https://doaj.org/toc/2076-2615<i>Blastocystis</i> sp. is an important zoonotic protist in humans and various animals with worldwide distribution. However, there have been no data on the occurrence of <i>Blastocystis</i> sp. in <i>C. bactrianus</i>, an important economic animal in northwestern China. In the present study, a PCR-sequencing tool based on the SSU rRNA gene was applied to investigate the prevalence and genetic diversity of <i>Blastocystis</i> sp. in 638 faecal samples from <i>C. bactrianus</i> in 21 sampling sites within three main breeding areas (Gansu, Inner Mongolia and Xinjiang) in northwestern China. The total prevalence of <i>Blastocystis</i> sp. was 21.8% (139/638) in <i>C. bactrianus</i>, with the infection rates of 29.5% (18/61), 50.0% (14/28) and 19.5% (107/549) for animals aged <2 years, 2–6 years and >6 years, respectively. Significant differences in prevalence were detected among <i>C. bactrianus</i> from three geographic areas (χ<sup>2</sup> = 19.972, <i>df</i> = 2, <i>p</i> < 0.001) and all sampling sites (χ<sup>2</sup> = 104.154, <i>df</i> = 20, <i>p</i> < 0.001). A total of 16 of 21 sampling sites were positive for <i>Blastocystis</i> sp., with the prevalence ranging from 7.7% to 70.6%. Sequence analysis of the SSU rRNA gene identified eight subtypes in <i>C. bactrianus</i> in the present study, including seven animal adapted subtypes (ST10, ST14, ST21, ST24, ST25, ST26 and ST30) and one potentially novel subtype, with ST10 being the dominant one. To the best of our knowledge, this study provides the first insight for the occurrence and genetic make-up of <i>Blastocystis</i> sp. in <i>C. bactrianus</i> and contributes to the understanding of the transmission of <i>Blastocystis</i> infection in <i>C. bactrianus</i> in China.Xin YangYunhui LiYuxin WangJunwei WangPeng LaiYuan LiJunke SongMeng QiGuanghui ZhaoMDPI AGarticle<i>Blastocystis</i> sp.<i>Camelus bactrianus</i>prevalencesubtypingnorthwestern ChinaVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3016, p 3016 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Blastocystis</i> sp. <i>Camelus bactrianus</i> prevalence subtyping northwestern China Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	<i>Blastocystis</i> sp. <i>Camelus bactrianus</i> prevalence subtyping northwestern China Veterinary medicine SF600-1100 Zoology QL1-991 Xin Yang Yunhui Li Yuxin Wang Junwei Wang Peng Lai Yuan Li Junke Song Meng Qi Guanghui Zhao Molecular Characterization of <i>Blastocystis</i> sp. in <i>Camelus bactrianus</i> in Northwestern China
description	<i>Blastocystis</i> sp. is an important zoonotic protist in humans and various animals with worldwide distribution. However, there have been no data on the occurrence of <i>Blastocystis</i> sp. in <i>C. bactrianus</i>, an important economic animal in northwestern China. In the present study, a PCR-sequencing tool based on the SSU rRNA gene was applied to investigate the prevalence and genetic diversity of <i>Blastocystis</i> sp. in 638 faecal samples from <i>C. bactrianus</i> in 21 sampling sites within three main breeding areas (Gansu, Inner Mongolia and Xinjiang) in northwestern China. The total prevalence of <i>Blastocystis</i> sp. was 21.8% (139/638) in <i>C. bactrianus</i>, with the infection rates of 29.5% (18/61), 50.0% (14/28) and 19.5% (107/549) for animals aged <2 years, 2–6 years and >6 years, respectively. Significant differences in prevalence were detected among <i>C. bactrianus</i> from three geographic areas (χ<sup>2</sup> = 19.972, <i>df</i> = 2, <i>p</i> < 0.001) and all sampling sites (χ<sup>2</sup> = 104.154, <i>df</i> = 20, <i>p</i> < 0.001). A total of 16 of 21 sampling sites were positive for <i>Blastocystis</i> sp., with the prevalence ranging from 7.7% to 70.6%. Sequence analysis of the SSU rRNA gene identified eight subtypes in <i>C. bactrianus</i> in the present study, including seven animal adapted subtypes (ST10, ST14, ST21, ST24, ST25, ST26 and ST30) and one potentially novel subtype, with ST10 being the dominant one. To the best of our knowledge, this study provides the first insight for the occurrence and genetic make-up of <i>Blastocystis</i> sp. in <i>C. bactrianus</i> and contributes to the understanding of the transmission of <i>Blastocystis</i> infection in <i>C. bactrianus</i> in China.
format	article
author	Xin Yang Yunhui Li Yuxin Wang Junwei Wang Peng Lai Yuan Li Junke Song Meng Qi Guanghui Zhao
author_facet	Xin Yang Yunhui Li Yuxin Wang Junwei Wang Peng Lai Yuan Li Junke Song Meng Qi Guanghui Zhao
author_sort	Xin Yang
title	Molecular Characterization of <i>Blastocystis</i> sp. in <i>Camelus bactrianus</i> in Northwestern China
title_short	Molecular Characterization of <i>Blastocystis</i> sp. in <i>Camelus bactrianus</i> in Northwestern China
title_full	Molecular Characterization of <i>Blastocystis</i> sp. in <i>Camelus bactrianus</i> in Northwestern China
title_fullStr	Molecular Characterization of <i>Blastocystis</i> sp. in <i>Camelus bactrianus</i> in Northwestern China
title_full_unstemmed	Molecular Characterization of <i>Blastocystis</i> sp. in <i>Camelus bactrianus</i> in Northwestern China
title_sort	molecular characterization of <i>blastocystis</i> sp. in <i>camelus bactrianus</i> in northwestern china
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2b31688561704dbf8dbf25641d6efa18
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Molecular Characterization of <i>Blastocystis</i> sp. in <i>Camelus bactrianus</i> in Northwestern China

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