iTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling

Abstract In plants, during growth and development, photoreceptors monitor fluctuations in their environment and adjust their metabolism as a strategy of surveillance. Phytochromes (Phys) play an essential role in plant growth and development, from germination to fruit development. FR-light (FR) inse...

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Autores principales:	Sherinmol Thomas, Rakesh Kumar, Kapil Sharma, Abhilash Barpanda, Yellamaraju Sreelakshmi, Rameshwar Sharma, Sanjeeva Srivastava
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b3386301a1845a8a818a516ff0c77f7
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spelling	oai:doaj.org-article:2b3386301a1845a8a818a516ff0c77f72021-12-02T14:26:51ZiTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling10.1038/s41598-021-87208-92045-2322https://doaj.org/article/2b3386301a1845a8a818a516ff0c77f72021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87208-9https://doaj.org/toc/2045-2322Abstract In plants, during growth and development, photoreceptors monitor fluctuations in their environment and adjust their metabolism as a strategy of surveillance. Phytochromes (Phys) play an essential role in plant growth and development, from germination to fruit development. FR-light (FR) insensitive mutant (fri) carries a recessive mutation in Phytochrome A and is characterized by the failure to de-etiolate in continuous FR. Here we used iTRAQ-based quantitative proteomics along with metabolomics to unravel the role of Phytochrome A in regulating central metabolism in tomato seedlings grown under FR. Our results indicate that Phytochrome A has a predominant role in FR-mediated establishment of the mature seedling proteome. Further, we observed temporal regulation in the expression of several of the late response proteins associated with central metabolism. The proteomics investigations identified a decreased abundance of enzymes involved in photosynthesis and carbon fixation in the mutant. Profound accumulation of storage proteins in the mutant ascertained the possible conversion of sugars into storage material instead of being used or the retention of an earlier profile associated with the mature embryo. The enhanced accumulation of organic sugars in the seedlings indicates the absence of photomorphogenesis in the mutant.Sherinmol ThomasRakesh KumarKapil SharmaAbhilash BarpandaYellamaraju SreelakshmiRameshwar SharmaSanjeeva SrivastavaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-21 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sherinmol Thomas Rakesh Kumar Kapil Sharma Abhilash Barpanda Yellamaraju Sreelakshmi Rameshwar Sharma Sanjeeva Srivastava iTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling
description	Abstract In plants, during growth and development, photoreceptors monitor fluctuations in their environment and adjust their metabolism as a strategy of surveillance. Phytochromes (Phys) play an essential role in plant growth and development, from germination to fruit development. FR-light (FR) insensitive mutant (fri) carries a recessive mutation in Phytochrome A and is characterized by the failure to de-etiolate in continuous FR. Here we used iTRAQ-based quantitative proteomics along with metabolomics to unravel the role of Phytochrome A in regulating central metabolism in tomato seedlings grown under FR. Our results indicate that Phytochrome A has a predominant role in FR-mediated establishment of the mature seedling proteome. Further, we observed temporal regulation in the expression of several of the late response proteins associated with central metabolism. The proteomics investigations identified a decreased abundance of enzymes involved in photosynthesis and carbon fixation in the mutant. Profound accumulation of storage proteins in the mutant ascertained the possible conversion of sugars into storage material instead of being used or the retention of an earlier profile associated with the mature embryo. The enhanced accumulation of organic sugars in the seedlings indicates the absence of photomorphogenesis in the mutant.
format	article
author	Sherinmol Thomas Rakesh Kumar Kapil Sharma Abhilash Barpanda Yellamaraju Sreelakshmi Rameshwar Sharma Sanjeeva Srivastava
author_facet	Sherinmol Thomas Rakesh Kumar Kapil Sharma Abhilash Barpanda Yellamaraju Sreelakshmi Rameshwar Sharma Sanjeeva Srivastava
author_sort	Sherinmol Thomas
title	iTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling
title_short	iTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling
title_full	iTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling
title_fullStr	iTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling
title_full_unstemmed	iTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling
title_sort	itraq-based proteome profiling revealed the role of phytochrome a in regulating primary metabolism in tomato seedling
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2b3386301a1845a8a818a516ff0c77f7
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iTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling

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