A modified consumer inkjet for spatiotemporal control of gene expression.

This paper presents a low-cost inkjet dosing system capable of continuous, two-dimensional spatiotemporal regulation of gene expression via delivery of diffusible regulators to a custom-mounted gel culture of E. coli. A consumer-grade, inkjet printer was adapted for chemical printing; E. coli cultur...

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Autores principales:	Daniel J Cohen, Roberto C Morfino, Michel M Maharbiz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b3d9cf6bc7f4c3b83be1aafa4b16090
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spelling	oai:doaj.org-article:2b3d9cf6bc7f4c3b83be1aafa4b160902021-11-25T06:20:18ZA modified consumer inkjet for spatiotemporal control of gene expression.1932-620310.1371/journal.pone.0007086https://doaj.org/article/2b3d9cf6bc7f4c3b83be1aafa4b160902009-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19763256/?tool=EBIhttps://doaj.org/toc/1932-6203This paper presents a low-cost inkjet dosing system capable of continuous, two-dimensional spatiotemporal regulation of gene expression via delivery of diffusible regulators to a custom-mounted gel culture of E. coli. A consumer-grade, inkjet printer was adapted for chemical printing; E. coli cultures were grown on 750 microm thick agar embedded in micro-wells machined into commercial compact discs. Spatio-temporal regulation of the lac operon was demonstrated via the printing of patterns of lactose and glucose directly into the cultures; X-Gal blue patterns were used for visual feedback. We demonstrate how the bistable nature of the lac operon's feedback, when perturbed by patterning lactose (inducer) and glucose (inhibitor), can lead to coordination of cell expression patterns across a field in ways that mimic motifs seen in developmental biology. Examples of this include sharp boundaries and the generation of traveling waves of mRNA expression. To our knowledge, this is the first demonstration of reaction-diffusion effects in the well-studied lac operon. A finite element reaction-diffusion model of the lac operon is also presented which predicts pattern formation with good fidelity.Daniel J CohenRoberto C MorfinoMichel M MaharbizPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 9, p e7086 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel J Cohen Roberto C Morfino Michel M Maharbiz A modified consumer inkjet for spatiotemporal control of gene expression.
description	This paper presents a low-cost inkjet dosing system capable of continuous, two-dimensional spatiotemporal regulation of gene expression via delivery of diffusible regulators to a custom-mounted gel culture of E. coli. A consumer-grade, inkjet printer was adapted for chemical printing; E. coli cultures were grown on 750 microm thick agar embedded in micro-wells machined into commercial compact discs. Spatio-temporal regulation of the lac operon was demonstrated via the printing of patterns of lactose and glucose directly into the cultures; X-Gal blue patterns were used for visual feedback. We demonstrate how the bistable nature of the lac operon's feedback, when perturbed by patterning lactose (inducer) and glucose (inhibitor), can lead to coordination of cell expression patterns across a field in ways that mimic motifs seen in developmental biology. Examples of this include sharp boundaries and the generation of traveling waves of mRNA expression. To our knowledge, this is the first demonstration of reaction-diffusion effects in the well-studied lac operon. A finite element reaction-diffusion model of the lac operon is also presented which predicts pattern formation with good fidelity.
format	article
author	Daniel J Cohen Roberto C Morfino Michel M Maharbiz
author_facet	Daniel J Cohen Roberto C Morfino Michel M Maharbiz
author_sort	Daniel J Cohen
title	A modified consumer inkjet for spatiotemporal control of gene expression.
title_short	A modified consumer inkjet for spatiotemporal control of gene expression.
title_full	A modified consumer inkjet for spatiotemporal control of gene expression.
title_fullStr	A modified consumer inkjet for spatiotemporal control of gene expression.
title_full_unstemmed	A modified consumer inkjet for spatiotemporal control of gene expression.
title_sort	modified consumer inkjet for spatiotemporal control of gene expression.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/2b3d9cf6bc7f4c3b83be1aafa4b16090
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_version_	1718413794606055424

A modified consumer inkjet for spatiotemporal control of gene expression.

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