Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase

Abstract AMP-activated protein kinase (AMPK) is a key sensor and regulator of glucose metabolism. Here, we demonstrated that shizukaol F, a natural compound isolated from Chloranthus japonicus, can activate AMPK and modulate glucose metabolism both in vitro and in vivo. Shizukaol F increased glucose...

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Autores principales:	Rongkuan Hu, Huan Yan, Xiaoyan Fei, Haiyang Liu, Jiarui Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b4a471feba04b03ade6e6be5bcc225e
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spelling	oai:doaj.org-article:2b4a471feba04b03ade6e6be5bcc225e2021-12-02T11:53:11ZModulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase10.1038/s41598-017-00925-y2045-2322https://doaj.org/article/2b4a471feba04b03ade6e6be5bcc225e2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00925-yhttps://doaj.org/toc/2045-2322Abstract AMP-activated protein kinase (AMPK) is a key sensor and regulator of glucose metabolism. Here, we demonstrated that shizukaol F, a natural compound isolated from Chloranthus japonicus, can activate AMPK and modulate glucose metabolism both in vitro and in vivo. Shizukaol F increased glucose uptake in differentiated C2C12 myotubes by stimulating glucose transporter-4 (GLUT-4) membraned translocation. Treatment of primary mouse hepatocytes with shizukaol F decreased the expression of phosphoenolpyruvate carboxykinase 2 (PEPCK), glucose-6-phosphatase (G6Pase) and suppressed hepatic gluconeogenesis. Meanwhile, a single oral dose of shizukaol F reduced gluconeogenesis in C57BL/6 J mice. Further studies indicated that shizukaol F modulates glucose metabolism mainly by AMPKa phosphorylation activity. In addition, we also found that shizukaol F depolarizes the mitochondrial membrane and inhibits respiratory complex I, which may result in AMPK activation. Our results highlight the potential value of shizukaol F as a possible treatment of metabolic syndrome.Rongkuan HuHuan YanXiaoyan FeiHaiyang LiuJiarui WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rongkuan Hu Huan Yan Xiaoyan Fei Haiyang Liu Jiarui Wu Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase
description	Abstract AMP-activated protein kinase (AMPK) is a key sensor and regulator of glucose metabolism. Here, we demonstrated that shizukaol F, a natural compound isolated from Chloranthus japonicus, can activate AMPK and modulate glucose metabolism both in vitro and in vivo. Shizukaol F increased glucose uptake in differentiated C2C12 myotubes by stimulating glucose transporter-4 (GLUT-4) membraned translocation. Treatment of primary mouse hepatocytes with shizukaol F decreased the expression of phosphoenolpyruvate carboxykinase 2 (PEPCK), glucose-6-phosphatase (G6Pase) and suppressed hepatic gluconeogenesis. Meanwhile, a single oral dose of shizukaol F reduced gluconeogenesis in C57BL/6 J mice. Further studies indicated that shizukaol F modulates glucose metabolism mainly by AMPKa phosphorylation activity. In addition, we also found that shizukaol F depolarizes the mitochondrial membrane and inhibits respiratory complex I, which may result in AMPK activation. Our results highlight the potential value of shizukaol F as a possible treatment of metabolic syndrome.
format	article
author	Rongkuan Hu Huan Yan Xiaoyan Fei Haiyang Liu Jiarui Wu
author_facet	Rongkuan Hu Huan Yan Xiaoyan Fei Haiyang Liu Jiarui Wu
author_sort	Rongkuan Hu
title	Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase
title_short	Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase
title_full	Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase
title_fullStr	Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase
title_full_unstemmed	Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase
title_sort	modulation of glucose metabolism by a natural compound from chloranthus japonicus via activation of amp-activated protein kinase
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2b4a471feba04b03ade6e6be5bcc225e
work_keys_str_mv	AT rongkuanhu modulationofglucosemetabolismbyanaturalcompoundfromchloranthusjaponicusviaactivationofampactivatedproteinkinase AT huanyan modulationofglucosemetabolismbyanaturalcompoundfromchloranthusjaponicusviaactivationofampactivatedproteinkinase AT xiaoyanfei modulationofglucosemetabolismbyanaturalcompoundfromchloranthusjaponicusviaactivationofampactivatedproteinkinase AT haiyangliu modulationofglucosemetabolismbyanaturalcompoundfromchloranthusjaponicusviaactivationofampactivatedproteinkinase AT jiaruiwu modulationofglucosemetabolismbyanaturalcompoundfromchloranthusjaponicusviaactivationofampactivatedproteinkinase
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Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase

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