Rimklb mutation causes male infertility in mice

Abstract Rimklb is a mammalian homologue of the E. coli enzyme RimK, which catalyzes addition of glutamic acid to the ribosomal protein S6. To date, no previous studies have shown any physiological role for Rimklb in mammals. In this study, using Western blotting, we found that Rimklb is distributed...

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Autores principales:	Koji Maekura, Satoshi Tsukamoto, Michiko Hamada-Kanazawa, Masaoki Takano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b5c9f27fc6b472b986ee9fa1344c85e
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spelling	oai:doaj.org-article:2b5c9f27fc6b472b986ee9fa1344c85e2021-12-02T13:34:46ZRimklb mutation causes male infertility in mice10.1038/s41598-021-84105-z2045-2322https://doaj.org/article/2b5c9f27fc6b472b986ee9fa1344c85e2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84105-zhttps://doaj.org/toc/2045-2322Abstract Rimklb is a mammalian homologue of the E. coli enzyme RimK, which catalyzes addition of glutamic acid to the ribosomal protein S6. To date, no previous studies have shown any physiological role for Rimklb in mammals. In this study, using Western blotting, we found that Rimklb is distributed and expressed in mouse testis and heart. Rimklb was subsequently localized to the testicular Leydig cells using immunohistochemistry with an anti-Rimklb antibody. We generated a Rimklb mutant mouse in which a three-base deletion results in deletion of Ala 29 and substitution of Leu 30 with Val, which we named the RimklbA29del, L30V mutant mouse. RimklbA29del, L30V mutant mice show a decrease in testicular size and weight, and in vitro fertilization demonstrates complete male infertility. Furthermore, we found that a key factor in the mammalian target of the rapamycin/ribosomal protein S6 transcriptional pathway is hyperphosphorylated in the seminiferous tubules of the mutant testis. We conclude that Rimklb has important roles that include spermatogenesis in seminiferous tubules. In summary, male RimklbA29del, L30V mice are infertile.Koji MaekuraSatoshi TsukamotoMichiko Hamada-KanazawaMasaoki TakanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Koji Maekura Satoshi Tsukamoto Michiko Hamada-Kanazawa Masaoki Takano Rimklb mutation causes male infertility in mice
description	Abstract Rimklb is a mammalian homologue of the E. coli enzyme RimK, which catalyzes addition of glutamic acid to the ribosomal protein S6. To date, no previous studies have shown any physiological role for Rimklb in mammals. In this study, using Western blotting, we found that Rimklb is distributed and expressed in mouse testis and heart. Rimklb was subsequently localized to the testicular Leydig cells using immunohistochemistry with an anti-Rimklb antibody. We generated a Rimklb mutant mouse in which a three-base deletion results in deletion of Ala 29 and substitution of Leu 30 with Val, which we named the RimklbA29del, L30V mutant mouse. RimklbA29del, L30V mutant mice show a decrease in testicular size and weight, and in vitro fertilization demonstrates complete male infertility. Furthermore, we found that a key factor in the mammalian target of the rapamycin/ribosomal protein S6 transcriptional pathway is hyperphosphorylated in the seminiferous tubules of the mutant testis. We conclude that Rimklb has important roles that include spermatogenesis in seminiferous tubules. In summary, male RimklbA29del, L30V mice are infertile.
format	article
author	Koji Maekura Satoshi Tsukamoto Michiko Hamada-Kanazawa Masaoki Takano
author_facet	Koji Maekura Satoshi Tsukamoto Michiko Hamada-Kanazawa Masaoki Takano
author_sort	Koji Maekura
title	Rimklb mutation causes male infertility in mice
title_short	Rimklb mutation causes male infertility in mice
title_full	Rimklb mutation causes male infertility in mice
title_fullStr	Rimklb mutation causes male infertility in mice
title_full_unstemmed	Rimklb mutation causes male infertility in mice
title_sort	rimklb mutation causes male infertility in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2b5c9f27fc6b472b986ee9fa1344c85e
work_keys_str_mv	AT kojimaekura rimklbmutationcausesmaleinfertilityinmice AT satoshitsukamoto rimklbmutationcausesmaleinfertilityinmice AT michikohamadakanazawa rimklbmutationcausesmaleinfertilityinmice AT masaokitakano rimklbmutationcausesmaleinfertilityinmice
_version_	1718392724784152576

Rimklb mutation causes male infertility in mice

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