Systematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafish

Abstract Long intergenic noncoding RNAs (lincRNAs) are increasingly recognized as potential key regulators of heart development and related diseases, but their identities and functions remain elusive. In this study, we sought to identify and characterize the cardiac lincRNA transcriptome in the expe...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lei Wang, Xiao Ma, Xiaolei Xu, Yuji Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b5d1d70029849068fbcf45bfa68912b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2b5d1d70029849068fbcf45bfa68912b
record_format	dspace
spelling	oai:doaj.org-article:2b5d1d70029849068fbcf45bfa68912b2021-12-02T16:07:47ZSystematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafish10.1038/s41598-017-00823-32045-2322https://doaj.org/article/2b5d1d70029849068fbcf45bfa68912b2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00823-3https://doaj.org/toc/2045-2322Abstract Long intergenic noncoding RNAs (lincRNAs) are increasingly recognized as potential key regulators of heart development and related diseases, but their identities and functions remain elusive. In this study, we sought to identify and characterize the cardiac lincRNA transcriptome in the experimentally accessible zebrafish model by integrating bioinformatics analysis with experimental validation. By conducting genome-wide RNA sequencing profiling of zebrafish embryonic hearts, adult hearts, and adult muscle, we generated a high-confidence set of 813 cardiac lincRNA transcripts, 423 of which are novel. Among these lincRNAs, 564 are expressed in the embryonic heart, and 730 are expressed in the adult heart, including 2 novel lincRNAs, TCONS_00000891 and TCONS_00028686, which exhibit cardiac-enriched expression patterns in adult heart. Using a method similar to a fetal gene program, we identified 51 lincRNAs with differential expression patterns between embryonic and adult hearts, among which TCONS_00009015 responded to doxorubicin-induced cardiac stress. In summary, our genome-wide systematic identification and characterization of cardiac lincRNAs lays the foundation for future studies in this vertebrate model to elucidate crucial roles for cardiac lincRNAs during heart development and cardiac diseases.Lei WangXiao MaXiaolei XuYuji ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lei Wang Xiao Ma Xiaolei Xu Yuji Zhang Systematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafish
description	Abstract Long intergenic noncoding RNAs (lincRNAs) are increasingly recognized as potential key regulators of heart development and related diseases, but their identities and functions remain elusive. In this study, we sought to identify and characterize the cardiac lincRNA transcriptome in the experimentally accessible zebrafish model by integrating bioinformatics analysis with experimental validation. By conducting genome-wide RNA sequencing profiling of zebrafish embryonic hearts, adult hearts, and adult muscle, we generated a high-confidence set of 813 cardiac lincRNA transcripts, 423 of which are novel. Among these lincRNAs, 564 are expressed in the embryonic heart, and 730 are expressed in the adult heart, including 2 novel lincRNAs, TCONS_00000891 and TCONS_00028686, which exhibit cardiac-enriched expression patterns in adult heart. Using a method similar to a fetal gene program, we identified 51 lincRNAs with differential expression patterns between embryonic and adult hearts, among which TCONS_00009015 responded to doxorubicin-induced cardiac stress. In summary, our genome-wide systematic identification and characterization of cardiac lincRNAs lays the foundation for future studies in this vertebrate model to elucidate crucial roles for cardiac lincRNAs during heart development and cardiac diseases.
format	article
author	Lei Wang Xiao Ma Xiaolei Xu Yuji Zhang
author_facet	Lei Wang Xiao Ma Xiaolei Xu Yuji Zhang
author_sort	Lei Wang
title	Systematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafish
title_short	Systematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafish
title_full	Systematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafish
title_fullStr	Systematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafish
title_full_unstemmed	Systematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafish
title_sort	systematic identification and characterization of cardiac long intergenic noncoding rnas in zebrafish
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2b5d1d70029849068fbcf45bfa68912b
work_keys_str_mv	AT leiwang systematicidentificationandcharacterizationofcardiaclongintergenicnoncodingrnasinzebrafish AT xiaoma systematicidentificationandcharacterizationofcardiaclongintergenicnoncodingrnasinzebrafish AT xiaoleixu systematicidentificationandcharacterizationofcardiaclongintergenicnoncodingrnasinzebrafish AT yujizhang systematicidentificationandcharacterizationofcardiaclongintergenicnoncodingrnasinzebrafish
_version_	1718384707430776832

Systematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafish

Ejemplares similares