Epigenetic Regulation of miR-92a and TET2 and Their Association in Non-Hodgkin Lymphoma

MicroRNAs (miRNAs) are well known for their ability to regulate the expression of specific target genes through degradation or inhibition of translation of the target mRNA. In various cancers, miRNAs regulate gene expression by altering the epigenetic status of candidate genes that are implicated in...

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Autores principales:	Esther K. Elliott, Lloyd N. Hopkins, Robert Hensen, Heidi G. Sutherland, Larisa M. Haupt, Lyn R. Griffiths
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	miR-17∼92 Burkitt lymphoma mantle cell lymphoma diffuse large B-cell lymphoma micro-RNA (miRNA/miR) DNA methylation Genetics QH426-470
Acceso en línea:	https://doaj.org/article/2b77846fad2b44fc839d3d50817ed005
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spelling	oai:doaj.org-article:2b77846fad2b44fc839d3d50817ed0052021-12-01T07:15:43ZEpigenetic Regulation of miR-92a and TET2 and Their Association in Non-Hodgkin Lymphoma1664-802110.3389/fgene.2021.768913https://doaj.org/article/2b77846fad2b44fc839d3d50817ed0052021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.768913/fullhttps://doaj.org/toc/1664-8021MicroRNAs (miRNAs) are well known for their ability to regulate the expression of specific target genes through degradation or inhibition of translation of the target mRNA. In various cancers, miRNAs regulate gene expression by altering the epigenetic status of candidate genes that are implicated in various difficult to treat haematological malignancies such as non-Hodgkin lymphoma by acting as either oncogenes or tumour suppressor genes. Cellular and circulating miRNA biomarkers could also be directly utilised as disease markers for diagnosis and monitoring of non-Hodgkin lymphoma (NHL); however, the role of DNA methylation in miRNA expression regulation in NHL requires further scientific inquiry. In this study, we investigated the methylation levels of CpGs in CpG islands spanning the promoter regions of the miR-17–92 cluster host gene and the TET2 gene and correlated them with the expression levels of TET2 mRNA and miR-92a-3p and miR-92a-5p mature miRNAs in NHL cell lines, tumour samples, and the whole blood gDNA of an NHL case control cohort. Increased expression of both miR-92a-3p and miR-92a-5p and aberrant expression of TET2 was observed in NHL cell lines and tumour tissues, as well as disparate levels of dysfunctional promoter CGI methylation. Both miR-92a and TET2 may play a concerted role in NHL malignancy and disease pathogenesis.Esther K. ElliottEsther K. ElliottLloyd N. HopkinsRobert HensenHeidi G. SutherlandLarisa M. HauptLyn R. GriffithsFrontiers Media S.A.articlemiR-17∼92Burkitt lymphomamantle cell lymphomadiffuse large B-cell lymphomamicro-RNA (miRNA/miR)DNA methylationGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	miR-17∼92 Burkitt lymphoma mantle cell lymphoma diffuse large B-cell lymphoma micro-RNA (miRNA/miR) DNA methylation Genetics QH426-470
spellingShingle	miR-17∼92 Burkitt lymphoma mantle cell lymphoma diffuse large B-cell lymphoma micro-RNA (miRNA/miR) DNA methylation Genetics QH426-470 Esther K. Elliott Esther K. Elliott Lloyd N. Hopkins Robert Hensen Heidi G. Sutherland Larisa M. Haupt Lyn R. Griffiths Epigenetic Regulation of miR-92a and TET2 and Their Association in Non-Hodgkin Lymphoma
description	MicroRNAs (miRNAs) are well known for their ability to regulate the expression of specific target genes through degradation or inhibition of translation of the target mRNA. In various cancers, miRNAs regulate gene expression by altering the epigenetic status of candidate genes that are implicated in various difficult to treat haematological malignancies such as non-Hodgkin lymphoma by acting as either oncogenes or tumour suppressor genes. Cellular and circulating miRNA biomarkers could also be directly utilised as disease markers for diagnosis and monitoring of non-Hodgkin lymphoma (NHL); however, the role of DNA methylation in miRNA expression regulation in NHL requires further scientific inquiry. In this study, we investigated the methylation levels of CpGs in CpG islands spanning the promoter regions of the miR-17–92 cluster host gene and the TET2 gene and correlated them with the expression levels of TET2 mRNA and miR-92a-3p and miR-92a-5p mature miRNAs in NHL cell lines, tumour samples, and the whole blood gDNA of an NHL case control cohort. Increased expression of both miR-92a-3p and miR-92a-5p and aberrant expression of TET2 was observed in NHL cell lines and tumour tissues, as well as disparate levels of dysfunctional promoter CGI methylation. Both miR-92a and TET2 may play a concerted role in NHL malignancy and disease pathogenesis.
format	article
author	Esther K. Elliott Esther K. Elliott Lloyd N. Hopkins Robert Hensen Heidi G. Sutherland Larisa M. Haupt Lyn R. Griffiths
author_facet	Esther K. Elliott Esther K. Elliott Lloyd N. Hopkins Robert Hensen Heidi G. Sutherland Larisa M. Haupt Lyn R. Griffiths
author_sort	Esther K. Elliott
title	Epigenetic Regulation of miR-92a and TET2 and Their Association in Non-Hodgkin Lymphoma
title_short	Epigenetic Regulation of miR-92a and TET2 and Their Association in Non-Hodgkin Lymphoma
title_full	Epigenetic Regulation of miR-92a and TET2 and Their Association in Non-Hodgkin Lymphoma
title_fullStr	Epigenetic Regulation of miR-92a and TET2 and Their Association in Non-Hodgkin Lymphoma
title_full_unstemmed	Epigenetic Regulation of miR-92a and TET2 and Their Association in Non-Hodgkin Lymphoma
title_sort	epigenetic regulation of mir-92a and tet2 and their association in non-hodgkin lymphoma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2b77846fad2b44fc839d3d50817ed005
work_keys_str_mv	AT estherkelliott epigeneticregulationofmir92aandtet2andtheirassociationinnonhodgkinlymphoma AT estherkelliott epigeneticregulationofmir92aandtet2andtheirassociationinnonhodgkinlymphoma AT lloydnhopkins epigeneticregulationofmir92aandtet2andtheirassociationinnonhodgkinlymphoma AT roberthensen epigeneticregulationofmir92aandtet2andtheirassociationinnonhodgkinlymphoma AT heidigsutherland epigeneticregulationofmir92aandtet2andtheirassociationinnonhodgkinlymphoma AT larisamhaupt epigeneticregulationofmir92aandtet2andtheirassociationinnonhodgkinlymphoma AT lynrgriffiths epigeneticregulationofmir92aandtet2andtheirassociationinnonhodgkinlymphoma
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Epigenetic Regulation of miR-92a and TET2 and Their Association in Non-Hodgkin Lymphoma

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