Discovery and Community Dynamics of Novel ssRNA Mycoviruses in the Conifer Pathogen Heterobasidion parviporum

Heterobasidion species are highly destructive basidiomycetous conifer pathogens of the Boreal forest region. Earlier studies have revealed dsRNA virus infections of families Curvulaviridae and Partitiviridae in Heterobasidion strains, and small RNA deep sequencing has also identified infections of M...

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Autores principales:	Suvi Sutela, Tuula Piri, Eeva J. Vainio
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	mycovirus forest pathogen boreal forest virus population Lenarviricota ambisense virus Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2b7aebfd300a4718a8b34d832859c177
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spelling	oai:doaj.org-article:2b7aebfd300a4718a8b34d832859c1772021-11-30T18:29:27ZDiscovery and Community Dynamics of Novel ssRNA Mycoviruses in the Conifer Pathogen Heterobasidion parviporum1664-302X10.3389/fmicb.2021.770787https://doaj.org/article/2b7aebfd300a4718a8b34d832859c1772021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.770787/fullhttps://doaj.org/toc/1664-302XHeterobasidion species are highly destructive basidiomycetous conifer pathogens of the Boreal forest region. Earlier studies have revealed dsRNA virus infections of families Curvulaviridae and Partitiviridae in Heterobasidion strains, and small RNA deep sequencing has also identified infections of Mitoviridae members in these fungi. In this study, the virome of Heterobasidion parviporum was examined for the first time by RNA-Seq using total RNA depleted of rRNA. This method successfully revealed new viruses representing two established (+)ssRNA virus families not found earlier in Heterobasidion: Narnaviridae and Botourmiaviridae. In addition, we identified the presence of a recently described virus group tentatively named “ambiviruses” in H. parviporum. The H. parviporum isolates included in the study originated from experimental forest sites located within 0.7 km range from each other, and a population analysis including 43 isolates was conducted at one of the experimental plots to establish the prevalence of the newly identified viruses in clonally spreading H. parviporum individuals. Our results indicate that viral infections are considerably more diverse and common among Heterobasidion isolates than known earlier and include ssRNA viruses with high prevalence and interspecies variation.Suvi SutelaTuula PiriEeva J. VainioFrontiers Media S.A.articlemycovirusforest pathogenboreal forestvirus populationLenarviricotaambisense virusMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mycovirus forest pathogen boreal forest virus population Lenarviricota ambisense virus Microbiology QR1-502
spellingShingle	mycovirus forest pathogen boreal forest virus population Lenarviricota ambisense virus Microbiology QR1-502 Suvi Sutela Tuula Piri Eeva J. Vainio Discovery and Community Dynamics of Novel ssRNA Mycoviruses in the Conifer Pathogen Heterobasidion parviporum
description	Heterobasidion species are highly destructive basidiomycetous conifer pathogens of the Boreal forest region. Earlier studies have revealed dsRNA virus infections of families Curvulaviridae and Partitiviridae in Heterobasidion strains, and small RNA deep sequencing has also identified infections of Mitoviridae members in these fungi. In this study, the virome of Heterobasidion parviporum was examined for the first time by RNA-Seq using total RNA depleted of rRNA. This method successfully revealed new viruses representing two established (+)ssRNA virus families not found earlier in Heterobasidion: Narnaviridae and Botourmiaviridae. In addition, we identified the presence of a recently described virus group tentatively named “ambiviruses” in H. parviporum. The H. parviporum isolates included in the study originated from experimental forest sites located within 0.7 km range from each other, and a population analysis including 43 isolates was conducted at one of the experimental plots to establish the prevalence of the newly identified viruses in clonally spreading H. parviporum individuals. Our results indicate that viral infections are considerably more diverse and common among Heterobasidion isolates than known earlier and include ssRNA viruses with high prevalence and interspecies variation.
format	article
author	Suvi Sutela Tuula Piri Eeva J. Vainio
author_facet	Suvi Sutela Tuula Piri Eeva J. Vainio
author_sort	Suvi Sutela
title	Discovery and Community Dynamics of Novel ssRNA Mycoviruses in the Conifer Pathogen Heterobasidion parviporum
title_short	Discovery and Community Dynamics of Novel ssRNA Mycoviruses in the Conifer Pathogen Heterobasidion parviporum
title_full	Discovery and Community Dynamics of Novel ssRNA Mycoviruses in the Conifer Pathogen Heterobasidion parviporum
title_fullStr	Discovery and Community Dynamics of Novel ssRNA Mycoviruses in the Conifer Pathogen Heterobasidion parviporum
title_full_unstemmed	Discovery and Community Dynamics of Novel ssRNA Mycoviruses in the Conifer Pathogen Heterobasidion parviporum
title_sort	discovery and community dynamics of novel ssrna mycoviruses in the conifer pathogen heterobasidion parviporum
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2b7aebfd300a4718a8b34d832859c177
work_keys_str_mv	AT suvisutela discoveryandcommunitydynamicsofnovelssrnamycovirusesintheconiferpathogenheterobasidionparviporum AT tuulapiri discoveryandcommunitydynamicsofnovelssrnamycovirusesintheconiferpathogenheterobasidionparviporum AT eevajvainio discoveryandcommunitydynamicsofnovelssrnamycovirusesintheconiferpathogenheterobasidionparviporum
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Discovery and Community Dynamics of Novel ssRNA Mycoviruses in the Conifer Pathogen Heterobasidion parviporum

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