QTL mapping and candidate gene analysis of seed vigor-related traits during artificial aging in wheat (Triticum aestivum)

Abstract High vigor seeds have greater yield potential than those with low vigor; however, long-term storage leads to a decline in this trait. The objective of this study was to identify quantitative trait loci (QTLs) for seed vigor-related traits under artificial aging conditions using a high-densi...

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Autores principales:	Huawei Shi, Wanghui Guan, Yugang Shi, Shuguang Wang, Hua Fan, Jinwen Yang, Weiguo Chen, Wenjun Zhang, Daizhen Sun, Ruilian Jing
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Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b83b4ea72fa4274b466e02239d64b78
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spelling	oai:doaj.org-article:2b83b4ea72fa4274b466e02239d64b782021-12-02T13:58:15ZQTL mapping and candidate gene analysis of seed vigor-related traits during artificial aging in wheat (Triticum aestivum)10.1038/s41598-020-75778-z2045-2322https://doaj.org/article/2b83b4ea72fa4274b466e02239d64b782020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-75778-zhttps://doaj.org/toc/2045-2322Abstract High vigor seeds have greater yield potential than those with low vigor; however, long-term storage leads to a decline in this trait. The objective of this study was to identify quantitative trait loci (QTLs) for seed vigor-related traits under artificial aging conditions using a high-density genetic linkage map of wheat (Triticum aestivum) and mine the related candidate genes. A doubled haploid population, derived from a cross between Hanxuan 10 × Lumai 14, was used as the experimental material. Six controlled-environment treatments were set up, i.e. the seeds were aged for 0, 24, 36, 48, 60, and 72 h at a high temperature (48 °C) and under high humidity (relative humidity 100%). Eight traits including seed germination percentage, germination energy, germination index, seedling length, root length, seedling weight, vigor index, and simple vigor index were measured. With the prolongation of artificial aging treatment, these traits showed a continuous downward trend and significant correlations were observed between most of them. A total of 49 additive QTLs for seed vigor-related traits were mapped onto 12 chromosomes (1B, 2D, 3A, 3B, 3D, 4A, 4D, 5A, 5B, 5D, 6D, and 7A); and each one accounted for 6.01–17.18% of the phenotypic variations. Twenty-five pairs of epistatic QTLs were detected on all chromosomes, except for 5D, 6A, and 7D, and each epistasis accounted for 7.35–26.06% of the phenotypic variations. Three additive QTL hot spots were found on chromosomes 5A, 5B, and 5D, respectively. 13 QTLs, QGEe5B, QGIe5B, QSLc5B, QSLd5B, QSLf5B, QRLd5B, QRLe5B, QRLf5B, QVId5B, QVIe5B, QVIf5B, QSVId5B, and QSVIe5B, were located in the marker interval AX-94643729 ~ AX-110529646 on 5B and the physical interval 707,412,449–710,959,479 bp. Genes including TRAESCS5B01G564900, TRAESCS5B01G564200, TRAESCS5B01G562600, TraesCS5B02G562700, TRAESCS5B01G561300, TRAESCS5B01G561400, and TRAESCS5B01G562100, located in this marker interval, were found to be involved in regulating the processes of carbohydrate and lipid metabolism, transcription, and cell division during the germination of aging seeds, thus they were viewed as candidate genes for seed viability-related traits. These findings provide the basis for the seed-based cloning and functional identification of related candidate genes for seed vigor.Huawei ShiWanghui GuanYugang ShiShuguang WangHua FanJinwen YangWeiguo ChenWenjun ZhangDaizhen SunRuilian JingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Huawei Shi Wanghui Guan Yugang Shi Shuguang Wang Hua Fan Jinwen Yang Weiguo Chen Wenjun Zhang Daizhen Sun Ruilian Jing QTL mapping and candidate gene analysis of seed vigor-related traits during artificial aging in wheat (Triticum aestivum)
description	Abstract High vigor seeds have greater yield potential than those with low vigor; however, long-term storage leads to a decline in this trait. The objective of this study was to identify quantitative trait loci (QTLs) for seed vigor-related traits under artificial aging conditions using a high-density genetic linkage map of wheat (Triticum aestivum) and mine the related candidate genes. A doubled haploid population, derived from a cross between Hanxuan 10 × Lumai 14, was used as the experimental material. Six controlled-environment treatments were set up, i.e. the seeds were aged for 0, 24, 36, 48, 60, and 72 h at a high temperature (48 °C) and under high humidity (relative humidity 100%). Eight traits including seed germination percentage, germination energy, germination index, seedling length, root length, seedling weight, vigor index, and simple vigor index were measured. With the prolongation of artificial aging treatment, these traits showed a continuous downward trend and significant correlations were observed between most of them. A total of 49 additive QTLs for seed vigor-related traits were mapped onto 12 chromosomes (1B, 2D, 3A, 3B, 3D, 4A, 4D, 5A, 5B, 5D, 6D, and 7A); and each one accounted for 6.01–17.18% of the phenotypic variations. Twenty-five pairs of epistatic QTLs were detected on all chromosomes, except for 5D, 6A, and 7D, and each epistasis accounted for 7.35–26.06% of the phenotypic variations. Three additive QTL hot spots were found on chromosomes 5A, 5B, and 5D, respectively. 13 QTLs, QGEe5B, QGIe5B, QSLc5B, QSLd5B, QSLf5B, QRLd5B, QRLe5B, QRLf5B, QVId5B, QVIe5B, QVIf5B, QSVId5B, and QSVIe5B, were located in the marker interval AX-94643729 ~ AX-110529646 on 5B and the physical interval 707,412,449–710,959,479 bp. Genes including TRAESCS5B01G564900, TRAESCS5B01G564200, TRAESCS5B01G562600, TraesCS5B02G562700, TRAESCS5B01G561300, TRAESCS5B01G561400, and TRAESCS5B01G562100, located in this marker interval, were found to be involved in regulating the processes of carbohydrate and lipid metabolism, transcription, and cell division during the germination of aging seeds, thus they were viewed as candidate genes for seed viability-related traits. These findings provide the basis for the seed-based cloning and functional identification of related candidate genes for seed vigor.
format	article
author	Huawei Shi Wanghui Guan Yugang Shi Shuguang Wang Hua Fan Jinwen Yang Weiguo Chen Wenjun Zhang Daizhen Sun Ruilian Jing
author_facet	Huawei Shi Wanghui Guan Yugang Shi Shuguang Wang Hua Fan Jinwen Yang Weiguo Chen Wenjun Zhang Daizhen Sun Ruilian Jing
author_sort	Huawei Shi
title	QTL mapping and candidate gene analysis of seed vigor-related traits during artificial aging in wheat (Triticum aestivum)
title_short	QTL mapping and candidate gene analysis of seed vigor-related traits during artificial aging in wheat (Triticum aestivum)
title_full	QTL mapping and candidate gene analysis of seed vigor-related traits during artificial aging in wheat (Triticum aestivum)
title_fullStr	QTL mapping and candidate gene analysis of seed vigor-related traits during artificial aging in wheat (Triticum aestivum)
title_full_unstemmed	QTL mapping and candidate gene analysis of seed vigor-related traits during artificial aging in wheat (Triticum aestivum)
title_sort	qtl mapping and candidate gene analysis of seed vigor-related traits during artificial aging in wheat (triticum aestivum)
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2b83b4ea72fa4274b466e02239d64b78
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QTL mapping and candidate gene analysis of seed vigor-related traits during artificial aging in wheat (Triticum aestivum)

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