Modification of composition of a nanoemulsion with different cholesteryl ester molecular species: Effects on stability, peroxidation, and cell uptake

Cristina P Almeida1, Carolina G Vital1, Thais C Contente1, Durvanei A Maria2, Raul C Maranh&atilde;o1,31Lipid Metabolism Laboratory, Heart Institute (InCor), Medical School Hospital, 2Biochemistry and Biophysics Laboratories, Butantan Institute, 3Faculty of Pharmaceutical Sciences, Universit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cristina P Almeida, Carolina G Vital, Thais C Contente, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2b83ec124af54181bf142d46e0ff47a3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2b83ec124af54181bf142d46e0ff47a3
record_format	dspace
spelling	oai:doaj.org-article:2b83ec124af54181bf142d46e0ff47a32021-12-02T01:13:35ZModification of composition of a nanoemulsion with different cholesteryl ester molecular species: Effects on stability, peroxidation, and cell uptake1176-91141178-2013https://doaj.org/article/2b83ec124af54181bf142d46e0ff47a32010-09-01T00:00:00Zhttp://www.dovepress.com/modification-of-composition-of-a-nanoemulsion-with-different-cholester-a5276https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Cristina P Almeida1, Carolina G Vital1, Thais C Contente1, Durvanei A Maria2, Raul C Maranh&atilde;o1,31Lipid Metabolism Laboratory, Heart Institute (InCor), Medical School Hospital, 2Biochemistry and Biophysics Laboratories, Butantan Institute, 3Faculty of Pharmaceutical Sciences, University of S&atilde;o Paulo, S&atilde;o Paulo, BrazilPurpose: Use of lipid nanoemulsions as carriers of drugs for therapeutic or diagnostic purposes has been increasingly studied. Here, it was tested whether modifications of core particle constitution could affect the characteristics and biologic properties of lipid nanoemulsions. Methods: Three nanoemulsions were prepared using cholesteryl oleate, cholesteryl stearate, or cholesteryl linoleate as main core constituents. Particle size, stability, pH, peroxidation of the nanoemulsions, and cell survival and uptake by different cell lines were evaluated.Results: It was shown that cholesteryl stearate nanoemulsions had the greatest particle size and all three nanoemulsions were stable during the 237-day observation period. The pH of the three nanoemulsion preparations tended to decrease over time, but the decrease in pH of cholesteryl stearate was smaller than that of cholesteryl oleate and cholesteryl linoleate. Lipoperoxidation was greater in cholesteryl linoleate than in cholesteryl oleate and cholesteryl stearate. After four hours&rsquo; incubation of human umbilical vein endothelial cells (HUVEC) with nanoemulsions, peroxidation was minimal in the presence of cholesteryl oleate and more pronounced with cholesteryl linoleate and cholesteryl stearate. In contrast, macrophage incubates showed the highest peroxidation rates with cholesteryl oleate. Cholesteryl linoleate induced the highest cell peroxidation rates, except in macrophages. Uptake of cholesteryl oleate nanoemulsion by HUVEC and fibroblasts was greater than that of cholesteryl linoleate and cholesteryl stearate. Uptake of the three nanoemulsions by monocytes was equal. Uptake of cholesteryl oleate and cholesteryl linoleate by macrophages was negligible, but macrophage uptake of cholesteryl stearate was higher. In H292 tumor cells, cholesteryl oleate showed the highest uptakes. HUVEC showed higher survival rates when incubated with cholesteryl stearate and smaller survival with cholesteryl linoleate. H292 survival was greater with cholesteryl stearate.Conclusion: Although all three nanoemulsion types were stable for a long period, considerable differences were observed in size, oxidation status, and cell survival and nanoemulsion uptake in all tested cell lines. Those differences may be helpful in protocol planning and interpretation of data from experiments with lipid nanoemulsions.Keywords: emulsions, cholesterol, drug vehicles, lipoprotein receptors, solid lipid nanoparticles Cristina P AlmeidaCarolina G VitalThais C Contenteet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 679-686 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Cristina P Almeida Carolina G Vital Thais C Contente et al Modification of composition of a nanoemulsion with different cholesteryl ester molecular species: Effects on stability, peroxidation, and cell uptake
description	Cristina P Almeida1, Carolina G Vital1, Thais C Contente1, Durvanei A Maria2, Raul C Maranh&atilde;o1,31Lipid Metabolism Laboratory, Heart Institute (InCor), Medical School Hospital, 2Biochemistry and Biophysics Laboratories, Butantan Institute, 3Faculty of Pharmaceutical Sciences, University of S&atilde;o Paulo, S&atilde;o Paulo, BrazilPurpose: Use of lipid nanoemulsions as carriers of drugs for therapeutic or diagnostic purposes has been increasingly studied. Here, it was tested whether modifications of core particle constitution could affect the characteristics and biologic properties of lipid nanoemulsions. Methods: Three nanoemulsions were prepared using cholesteryl oleate, cholesteryl stearate, or cholesteryl linoleate as main core constituents. Particle size, stability, pH, peroxidation of the nanoemulsions, and cell survival and uptake by different cell lines were evaluated.Results: It was shown that cholesteryl stearate nanoemulsions had the greatest particle size and all three nanoemulsions were stable during the 237-day observation period. The pH of the three nanoemulsion preparations tended to decrease over time, but the decrease in pH of cholesteryl stearate was smaller than that of cholesteryl oleate and cholesteryl linoleate. Lipoperoxidation was greater in cholesteryl linoleate than in cholesteryl oleate and cholesteryl stearate. After four hours&rsquo; incubation of human umbilical vein endothelial cells (HUVEC) with nanoemulsions, peroxidation was minimal in the presence of cholesteryl oleate and more pronounced with cholesteryl linoleate and cholesteryl stearate. In contrast, macrophage incubates showed the highest peroxidation rates with cholesteryl oleate. Cholesteryl linoleate induced the highest cell peroxidation rates, except in macrophages. Uptake of cholesteryl oleate nanoemulsion by HUVEC and fibroblasts was greater than that of cholesteryl linoleate and cholesteryl stearate. Uptake of the three nanoemulsions by monocytes was equal. Uptake of cholesteryl oleate and cholesteryl linoleate by macrophages was negligible, but macrophage uptake of cholesteryl stearate was higher. In H292 tumor cells, cholesteryl oleate showed the highest uptakes. HUVEC showed higher survival rates when incubated with cholesteryl stearate and smaller survival with cholesteryl linoleate. H292 survival was greater with cholesteryl stearate.Conclusion: Although all three nanoemulsion types were stable for a long period, considerable differences were observed in size, oxidation status, and cell survival and nanoemulsion uptake in all tested cell lines. Those differences may be helpful in protocol planning and interpretation of data from experiments with lipid nanoemulsions.Keywords: emulsions, cholesterol, drug vehicles, lipoprotein receptors, solid lipid nanoparticles
format	article
author	Cristina P Almeida Carolina G Vital Thais C Contente et al
author_facet	Cristina P Almeida Carolina G Vital Thais C Contente et al
author_sort	Cristina P Almeida
title	Modification of composition of a nanoemulsion with different cholesteryl ester molecular species: Effects on stability, peroxidation, and cell uptake
title_short	Modification of composition of a nanoemulsion with different cholesteryl ester molecular species: Effects on stability, peroxidation, and cell uptake
title_full	Modification of composition of a nanoemulsion with different cholesteryl ester molecular species: Effects on stability, peroxidation, and cell uptake
title_fullStr	Modification of composition of a nanoemulsion with different cholesteryl ester molecular species: Effects on stability, peroxidation, and cell uptake
title_full_unstemmed	Modification of composition of a nanoemulsion with different cholesteryl ester molecular species: Effects on stability, peroxidation, and cell uptake
title_sort	modification of composition of a nanoemulsion with different cholesteryl ester molecular species: effects on stability, peroxidation, and cell uptake
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/2b83ec124af54181bf142d46e0ff47a3
work_keys_str_mv	AT cristinapalmeida modificationofcompositionofananoemulsionwithdifferentcholesterylestermolecularspecieseffectsonstabilityperoxidationandcelluptake AT carolinagvital modificationofcompositionofananoemulsionwithdifferentcholesterylestermolecularspecieseffectsonstabilityperoxidationandcelluptake AT thaisccontente modificationofcompositionofananoemulsionwithdifferentcholesterylestermolecularspecieseffectsonstabilityperoxidationandcelluptake AT etal modificationofcompositionofananoemulsionwithdifferentcholesterylestermolecularspecieseffectsonstabilityperoxidationandcelluptake
_version_	1718403189448900608

Modification of composition of a nanoemulsion with different cholesteryl ester molecular species: Effects on stability, peroxidation, and cell uptake

Ejemplares similares