Effects of DHA dietary intervention on hepatic lipid metabolism in apolipoprotein E-deficient and C57BL/6J wild-type mice

Lipid metabolic disorder occurs when ApoE gene is deficient. However, the role of Docosahexaenoic acid (DHA) in relieving hepatic lipid metabolic disorder in apolipoprotein E-deficient (ApoE -/-) mice remains unknown. We fed 3-month-old C57BL/6J wild-type (C57 wt) and ApoE -/- mice respectively with...

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Autores principales:	Jingjing Xu, Yujie Guo, Xiaochen Huang, Xiaojun Ma, Pengfei Li, Ying Wang, Xixiang Wang, Linhong Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Apolipoprotein E-deficient mice DHA Lipid metabolism Liver Non-alcoholic fatty liver disease Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/2b8428607d034a9389da949bd8f9b16d
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spelling	oai:doaj.org-article:2b8428607d034a9389da949bd8f9b16d2021-11-14T04:29:46ZEffects of DHA dietary intervention on hepatic lipid metabolism in apolipoprotein E-deficient and C57BL/6J wild-type mice0753-332210.1016/j.biopha.2021.112329https://doaj.org/article/2b8428607d034a9389da949bd8f9b16d2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011136https://doaj.org/toc/0753-3322Lipid metabolic disorder occurs when ApoE gene is deficient. However, the role of Docosahexaenoic acid (DHA) in relieving hepatic lipid metabolic disorder in apolipoprotein E-deficient (ApoE -/-) mice remains unknown. We fed 3-month-old C57BL/6J wild-type (C57 wt) and ApoE -/- mice respectively with normal or DHA fortified diet for 5 months. We found ApoE gene deficiency caused hepatic lipid deposition and increased lipid levels in plasma and liver. Hepatic gene expression of SRB1, CD36 and FABP5 in ApoE -/- mice, protein expression of HMGCR, LRP1 in C57 wt mice and protein expression of LRP1 in ApoE -/- mice increased after DHA intervention. In DHA-fed ApoE -/- mice, LXRα/β and PPARα protein expression down-regulated in cytoplasm, but LXRα/β protein expression up-regulated in nucleus. DHA treatment decreased RXRα and RXRβ expression in C57 wt and ApoE -/- female mice. Deletion of ApoE gene caused lipid metabolism disorder in liver of mice. DHA treatment efficiently meliorated lipid metabolism caused by ApoE deficient through the regulation of gene and protein expressions of molecules involved in liver fatty acids transport and lipid metabolism.Jingjing XuYujie GuoXiaochen HuangXiaojun MaPengfei LiYing WangXixiang WangLinhong YuanElsevierarticleApolipoprotein E-deficient miceDHALipid metabolismLiverNon-alcoholic fatty liver diseaseTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112329- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apolipoprotein E-deficient mice DHA Lipid metabolism Liver Non-alcoholic fatty liver disease Therapeutics. Pharmacology RM1-950
spellingShingle	Apolipoprotein E-deficient mice DHA Lipid metabolism Liver Non-alcoholic fatty liver disease Therapeutics. Pharmacology RM1-950 Jingjing Xu Yujie Guo Xiaochen Huang Xiaojun Ma Pengfei Li Ying Wang Xixiang Wang Linhong Yuan Effects of DHA dietary intervention on hepatic lipid metabolism in apolipoprotein E-deficient and C57BL/6J wild-type mice
description	Lipid metabolic disorder occurs when ApoE gene is deficient. However, the role of Docosahexaenoic acid (DHA) in relieving hepatic lipid metabolic disorder in apolipoprotein E-deficient (ApoE -/-) mice remains unknown. We fed 3-month-old C57BL/6J wild-type (C57 wt) and ApoE -/- mice respectively with normal or DHA fortified diet for 5 months. We found ApoE gene deficiency caused hepatic lipid deposition and increased lipid levels in plasma and liver. Hepatic gene expression of SRB1, CD36 and FABP5 in ApoE -/- mice, protein expression of HMGCR, LRP1 in C57 wt mice and protein expression of LRP1 in ApoE -/- mice increased after DHA intervention. In DHA-fed ApoE -/- mice, LXRα/β and PPARα protein expression down-regulated in cytoplasm, but LXRα/β protein expression up-regulated in nucleus. DHA treatment decreased RXRα and RXRβ expression in C57 wt and ApoE -/- female mice. Deletion of ApoE gene caused lipid metabolism disorder in liver of mice. DHA treatment efficiently meliorated lipid metabolism caused by ApoE deficient through the regulation of gene and protein expressions of molecules involved in liver fatty acids transport and lipid metabolism.
format	article
author	Jingjing Xu Yujie Guo Xiaochen Huang Xiaojun Ma Pengfei Li Ying Wang Xixiang Wang Linhong Yuan
author_facet	Jingjing Xu Yujie Guo Xiaochen Huang Xiaojun Ma Pengfei Li Ying Wang Xixiang Wang Linhong Yuan
author_sort	Jingjing Xu
title	Effects of DHA dietary intervention on hepatic lipid metabolism in apolipoprotein E-deficient and C57BL/6J wild-type mice
title_short	Effects of DHA dietary intervention on hepatic lipid metabolism in apolipoprotein E-deficient and C57BL/6J wild-type mice
title_full	Effects of DHA dietary intervention on hepatic lipid metabolism in apolipoprotein E-deficient and C57BL/6J wild-type mice
title_fullStr	Effects of DHA dietary intervention on hepatic lipid metabolism in apolipoprotein E-deficient and C57BL/6J wild-type mice
title_full_unstemmed	Effects of DHA dietary intervention on hepatic lipid metabolism in apolipoprotein E-deficient and C57BL/6J wild-type mice
title_sort	effects of dha dietary intervention on hepatic lipid metabolism in apolipoprotein e-deficient and c57bl/6j wild-type mice
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2b8428607d034a9389da949bd8f9b16d
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Effects of DHA dietary intervention on hepatic lipid metabolism in apolipoprotein E-deficient and C57BL/6J wild-type mice

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