Physiological characteristics of Magnetospirillum gryphiswaldense MSR-1 that control cell growth under high-iron and low-oxygen conditions

Abstract Magnetosome formation by Magnetospirillum gryphiswaldense MSR-1 is dependent on iron and oxygen levels. We used transcriptome to evaluate transcriptional profiles of magnetic and non-magnetic MSR-1 cells cultured under high-iron and low-iron conditions. A total of 80 differentially expresse...

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Autores principales:	Qing Wang, Xu Wang, Weijia Zhang, Xianyu Li, Yuan Zhou, Dan Li, Yinjia Wang, Jiesheng Tian, Wei Jiang, Ziding Zhang, Youliang Peng, Lei Wang, Ying Li, Jilun Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b86b66f1e104ed2a1555325ac5084f9
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spelling	oai:doaj.org-article:2b86b66f1e104ed2a1555325ac5084f92021-12-02T15:05:33ZPhysiological characteristics of Magnetospirillum gryphiswaldense MSR-1 that control cell growth under high-iron and low-oxygen conditions10.1038/s41598-017-03012-42045-2322https://doaj.org/article/2b86b66f1e104ed2a1555325ac5084f92017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03012-4https://doaj.org/toc/2045-2322Abstract Magnetosome formation by Magnetospirillum gryphiswaldense MSR-1 is dependent on iron and oxygen levels. We used transcriptome to evaluate transcriptional profiles of magnetic and non-magnetic MSR-1 cells cultured under high-iron and low-iron conditions. A total of 80 differentially expressed genes (DEGs) were identified, including 53 upregulated and 27 downregulated under high-iron condition. These DEGs belonged to the functional categories of biological regulation, oxidation-reduction process, and ion binding and transport, and were involved in sulfur metabolism and cysteine/methionine metabolism. Comparison with our previous results from transcriptome data under oxygen-controlled conditions indicated that transcription of mam or mms was not regulated by oxygen or iron signals. 17 common DEGs in iron- and oxygen-transcriptomes were involved in energy production, iron transport, and iron metabolism. Some unknown-function DEGs participate in iron transport and metabolism, and some are potential biomarkers for identification of Magnetospirillum strains. IrrA and IrrB regulate iron transport in response to low-oxygen and high-iron signals, respectively. Six transcription factors were predicted to regulate DEGs. Fur and Crp particularly co-regulate DEGs in response to changes in iron or oxygen levels, in a proposed joint regulatory network of DEGs. Our findings provide new insights into biomineralization processes under high- vs. low-iron conditions in magnetotactic bacteria.Qing WangXu WangWeijia ZhangXianyu LiYuan ZhouDan LiYinjia WangJiesheng TianWei JiangZiding ZhangYouliang PengLei WangYing LiJilun LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qing Wang Xu Wang Weijia Zhang Xianyu Li Yuan Zhou Dan Li Yinjia Wang Jiesheng Tian Wei Jiang Ziding Zhang Youliang Peng Lei Wang Ying Li Jilun Li Physiological characteristics of Magnetospirillum gryphiswaldense MSR-1 that control cell growth under high-iron and low-oxygen conditions
description	Abstract Magnetosome formation by Magnetospirillum gryphiswaldense MSR-1 is dependent on iron and oxygen levels. We used transcriptome to evaluate transcriptional profiles of magnetic and non-magnetic MSR-1 cells cultured under high-iron and low-iron conditions. A total of 80 differentially expressed genes (DEGs) were identified, including 53 upregulated and 27 downregulated under high-iron condition. These DEGs belonged to the functional categories of biological regulation, oxidation-reduction process, and ion binding and transport, and were involved in sulfur metabolism and cysteine/methionine metabolism. Comparison with our previous results from transcriptome data under oxygen-controlled conditions indicated that transcription of mam or mms was not regulated by oxygen or iron signals. 17 common DEGs in iron- and oxygen-transcriptomes were involved in energy production, iron transport, and iron metabolism. Some unknown-function DEGs participate in iron transport and metabolism, and some are potential biomarkers for identification of Magnetospirillum strains. IrrA and IrrB regulate iron transport in response to low-oxygen and high-iron signals, respectively. Six transcription factors were predicted to regulate DEGs. Fur and Crp particularly co-regulate DEGs in response to changes in iron or oxygen levels, in a proposed joint regulatory network of DEGs. Our findings provide new insights into biomineralization processes under high- vs. low-iron conditions in magnetotactic bacteria.
format	article
author	Qing Wang Xu Wang Weijia Zhang Xianyu Li Yuan Zhou Dan Li Yinjia Wang Jiesheng Tian Wei Jiang Ziding Zhang Youliang Peng Lei Wang Ying Li Jilun Li
author_facet	Qing Wang Xu Wang Weijia Zhang Xianyu Li Yuan Zhou Dan Li Yinjia Wang Jiesheng Tian Wei Jiang Ziding Zhang Youliang Peng Lei Wang Ying Li Jilun Li
author_sort	Qing Wang
title	Physiological characteristics of Magnetospirillum gryphiswaldense MSR-1 that control cell growth under high-iron and low-oxygen conditions
title_short	Physiological characteristics of Magnetospirillum gryphiswaldense MSR-1 that control cell growth under high-iron and low-oxygen conditions
title_full	Physiological characteristics of Magnetospirillum gryphiswaldense MSR-1 that control cell growth under high-iron and low-oxygen conditions
title_fullStr	Physiological characteristics of Magnetospirillum gryphiswaldense MSR-1 that control cell growth under high-iron and low-oxygen conditions
title_full_unstemmed	Physiological characteristics of Magnetospirillum gryphiswaldense MSR-1 that control cell growth under high-iron and low-oxygen conditions
title_sort	physiological characteristics of magnetospirillum gryphiswaldense msr-1 that control cell growth under high-iron and low-oxygen conditions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2b86b66f1e104ed2a1555325ac5084f9
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Physiological characteristics of Magnetospirillum gryphiswaldense MSR-1 that control cell growth under high-iron and low-oxygen conditions

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