MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway

Abstract Exploring the expression of miR-571 in patients with liver fibrosis and its role in the progression of liver fibrosis. A total of 74 patients with liver fibrosis in our institution from September to December 2018 were collected for study, and the expression of miR-571, Notch3 and Jagged1 in...

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Autores principales:	Shuo Cong, Yongmei Liu, Yi Li, Yu Chen, Rui Chen, Baofang Zhang, Lei Yu, Yaxin Hu, Xueke Zhao, Mao Mu, Mingliang Cheng, Zhi Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b88ef43c3104f2bb7cad42ca929c2ef
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spelling	oai:doaj.org-article:2b88ef43c3104f2bb7cad42ca929c2ef2021-11-14T12:18:14ZMiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway10.1038/s41598-021-00638-32045-2322https://doaj.org/article/2b88ef43c3104f2bb7cad42ca929c2ef2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00638-3https://doaj.org/toc/2045-2322Abstract Exploring the expression of miR-571 in patients with liver fibrosis and its role in the progression of liver fibrosis. A total of 74 patients with liver fibrosis in our institution from September to December 2018 were collected for study, and the expression of miR-571, Notch3 and Jagged1 in patients with different progressions of liver fibrosis was determined by RT-PCR and Western blot analysis. Set up Notch3 up group and Notch3 down regulated group, RT-PCR and Western blot were used to determine the effect of Notch signaling on the expression of fibrogenic factors. CCK-8, cell scratch assays, Transwell assays, flow cytometry were used to determine the effect of miR-571 on LX-2 proliferation, migration, apoptosis in human stem stellate cells, and RT-PCR, Western blot assays were performed to determine the effect of miR-571 on the Notch3 signaling pathway and the expression of profibrogenic factors. miR-571, Notch3 and Jagged1 are up-regulated in patients with liver fibrosis and is associated with the progression of liver fibrosis. Notch3 signaling pathway can promote the expression of fibroblast in human hepatic stellate cells; miR-571 can inhibit the apoptosis of human hepatic stellate cells, promote cell proliferation and migration; up regulation of miR-571 can promote the expression of Notch3 and Jagged1, and up-regulation of miR-571 also promoted the expression of related fibroblasts. MiR-571 can promote the activation of human stem cell stellate cells and the expression of fibroblast related factors through Notch3 signaling pathway.Shuo CongYongmei LiuYi LiYu ChenRui ChenBaofang ZhangLei YuYaxin HuXueke ZhaoMao MuMingliang ChengZhi HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuo Cong Yongmei Liu Yi Li Yu Chen Rui Chen Baofang Zhang Lei Yu Yaxin Hu Xueke Zhao Mao Mu Mingliang Cheng Zhi Huang MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway
description	Abstract Exploring the expression of miR-571 in patients with liver fibrosis and its role in the progression of liver fibrosis. A total of 74 patients with liver fibrosis in our institution from September to December 2018 were collected for study, and the expression of miR-571, Notch3 and Jagged1 in patients with different progressions of liver fibrosis was determined by RT-PCR and Western blot analysis. Set up Notch3 up group and Notch3 down regulated group, RT-PCR and Western blot were used to determine the effect of Notch signaling on the expression of fibrogenic factors. CCK-8, cell scratch assays, Transwell assays, flow cytometry were used to determine the effect of miR-571 on LX-2 proliferation, migration, apoptosis in human stem stellate cells, and RT-PCR, Western blot assays were performed to determine the effect of miR-571 on the Notch3 signaling pathway and the expression of profibrogenic factors. miR-571, Notch3 and Jagged1 are up-regulated in patients with liver fibrosis and is associated with the progression of liver fibrosis. Notch3 signaling pathway can promote the expression of fibroblast in human hepatic stellate cells; miR-571 can inhibit the apoptosis of human hepatic stellate cells, promote cell proliferation and migration; up regulation of miR-571 can promote the expression of Notch3 and Jagged1, and up-regulation of miR-571 also promoted the expression of related fibroblasts. MiR-571 can promote the activation of human stem cell stellate cells and the expression of fibroblast related factors through Notch3 signaling pathway.
format	article
author	Shuo Cong Yongmei Liu Yi Li Yu Chen Rui Chen Baofang Zhang Lei Yu Yaxin Hu Xueke Zhao Mao Mu Mingliang Cheng Zhi Huang
author_facet	Shuo Cong Yongmei Liu Yi Li Yu Chen Rui Chen Baofang Zhang Lei Yu Yaxin Hu Xueke Zhao Mao Mu Mingliang Cheng Zhi Huang
author_sort	Shuo Cong
title	MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway
title_short	MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway
title_full	MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway
title_fullStr	MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway
title_full_unstemmed	MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway
title_sort	mir-571 affects the development and progression of liver fibrosis by regulating the notch3 pathway
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2b88ef43c3104f2bb7cad42ca929c2ef
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MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway

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