Pollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system.

Despite a great number of published studies addressing estuarine, freshwater and marine bacterial diversity, few have examined urban coastal lagoons in tropical habitats. There is an increasing interest in monitoring opportunistic pathogens as well as indigenous microbial community members in these...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gigliola R B Salloto, Alexander M Cardoso, Felipe H Coutinho, Leonardo H Pinto, Ricardo P Vieira, Catia Chaia, Joyce L Lima, Rodolpho M Albano, Orlando B Martins, Maysa M Clementino
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b8a81184a7644af8f181c57098fec35
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2b8a81184a7644af8f181c57098fec35
record_format	dspace
spelling	oai:doaj.org-article:2b8a81184a7644af8f181c57098fec352021-11-18T08:06:33ZPollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system.1932-620310.1371/journal.pone.0051175https://doaj.org/article/2b8a81184a7644af8f181c57098fec352012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226484/?tool=EBIhttps://doaj.org/toc/1932-6203Despite a great number of published studies addressing estuarine, freshwater and marine bacterial diversity, few have examined urban coastal lagoons in tropical habitats. There is an increasing interest in monitoring opportunistic pathogens as well as indigenous microbial community members in these water bodies by current molecular and microbiological approaches. In this work, bacterial isolates were obtained through selective plate dilution methods to evaluate antibiotic resistances. In addition, 16S rRNA gene libraries were prepared from environmental waters and mixed cultures grown in BHI medium inoculated with Jacarepaguá lagoon waters. Denaturing gradient gel electrophoresis (DGGE) analyses showed distinct community profiles between environmental communities from each studied site and their cultured counterparts. A total of 497 bacterial sequences were analyzed by MOTHUR, yielding 245 operational taxonomic units (OTUs) grouped at 97% similarity. CCA diagrams showcased how several environmental variables affect the distribution of 18 bacterial orders throughout the three distinct habitats. UniFrac metrics and Venn diagrams revealed that bacterial communities retrieved through each experimental approach were significantly different and that only one OTU, closely related to Vibrio cholerae, was shared between them. Potentially pathogenic bacteria were isolated from most sampled environments, fifty percent of which showed antibiotic resistance.Gigliola R B SallotoAlexander M CardosoFelipe H CoutinhoLeonardo H PintoRicardo P VieiraCatia ChaiaJoyce L LimaRodolpho M AlbanoOrlando B MartinsMaysa M ClementinoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e51175 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gigliola R B Salloto Alexander M Cardoso Felipe H Coutinho Leonardo H Pinto Ricardo P Vieira Catia Chaia Joyce L Lima Rodolpho M Albano Orlando B Martins Maysa M Clementino Pollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system.
description	Despite a great number of published studies addressing estuarine, freshwater and marine bacterial diversity, few have examined urban coastal lagoons in tropical habitats. There is an increasing interest in monitoring opportunistic pathogens as well as indigenous microbial community members in these water bodies by current molecular and microbiological approaches. In this work, bacterial isolates were obtained through selective plate dilution methods to evaluate antibiotic resistances. In addition, 16S rRNA gene libraries were prepared from environmental waters and mixed cultures grown in BHI medium inoculated with Jacarepaguá lagoon waters. Denaturing gradient gel electrophoresis (DGGE) analyses showed distinct community profiles between environmental communities from each studied site and their cultured counterparts. A total of 497 bacterial sequences were analyzed by MOTHUR, yielding 245 operational taxonomic units (OTUs) grouped at 97% similarity. CCA diagrams showcased how several environmental variables affect the distribution of 18 bacterial orders throughout the three distinct habitats. UniFrac metrics and Venn diagrams revealed that bacterial communities retrieved through each experimental approach were significantly different and that only one OTU, closely related to Vibrio cholerae, was shared between them. Potentially pathogenic bacteria were isolated from most sampled environments, fifty percent of which showed antibiotic resistance.
format	article
author	Gigliola R B Salloto Alexander M Cardoso Felipe H Coutinho Leonardo H Pinto Ricardo P Vieira Catia Chaia Joyce L Lima Rodolpho M Albano Orlando B Martins Maysa M Clementino
author_facet	Gigliola R B Salloto Alexander M Cardoso Felipe H Coutinho Leonardo H Pinto Ricardo P Vieira Catia Chaia Joyce L Lima Rodolpho M Albano Orlando B Martins Maysa M Clementino
author_sort	Gigliola R B Salloto
title	Pollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system.
title_short	Pollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system.
title_full	Pollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system.
title_fullStr	Pollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system.
title_full_unstemmed	Pollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system.
title_sort	pollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2b8a81184a7644af8f181c57098fec35
work_keys_str_mv	AT gigliolarbsalloto pollutionimpactsonbacterioplanktondiversityinatropicalurbancoastallagoonsystem AT alexandermcardoso pollutionimpactsonbacterioplanktondiversityinatropicalurbancoastallagoonsystem AT felipehcoutinho pollutionimpactsonbacterioplanktondiversityinatropicalurbancoastallagoonsystem AT leonardohpinto pollutionimpactsonbacterioplanktondiversityinatropicalurbancoastallagoonsystem AT ricardopvieira pollutionimpactsonbacterioplanktondiversityinatropicalurbancoastallagoonsystem AT catiachaia pollutionimpactsonbacterioplanktondiversityinatropicalurbancoastallagoonsystem AT joycellima pollutionimpactsonbacterioplanktondiversityinatropicalurbancoastallagoonsystem AT rodolphomalbano pollutionimpactsonbacterioplanktondiversityinatropicalurbancoastallagoonsystem AT orlandobmartins pollutionimpactsonbacterioplanktondiversityinatropicalurbancoastallagoonsystem AT maysamclementino pollutionimpactsonbacterioplanktondiversityinatropicalurbancoastallagoonsystem
_version_	1718422218253271040

Pollution impacts on bacterioplankton diversity in a tropical urban coastal lagoon system.

Ejemplares similares