Characterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus Encephalitis

Human parechovirus type 3 (PeV-A3) infection has been recognized as an emerging etiologic factor causing severe nerve disease or sepsis in infants and young children. But the neuropathogenic mechanisms of PeV-A3 remain unknown. To understand the pathogenesis of PeV-A3 infection in the neuronal syste...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ming-Wei Jan, Hong-Lin Su, Tsung-Hsien Chang, Kuen-Jer Tsai
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	parechovirus A3 cytopathic effect inflammation neuronal diseases infectious model Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/2b8c417aad7f48fb99cf8a71a04fcdc7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2b8c417aad7f48fb99cf8a71a04fcdc7
record_format	dspace
spelling	oai:doaj.org-article:2b8c417aad7f48fb99cf8a71a04fcdc72021-11-30T21:33:07ZCharacterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus Encephalitis1664-322410.3389/fimmu.2021.753683https://doaj.org/article/2b8c417aad7f48fb99cf8a71a04fcdc72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.753683/fullhttps://doaj.org/toc/1664-3224Human parechovirus type 3 (PeV-A3) infection has been recognized as an emerging etiologic factor causing severe nerve disease or sepsis in infants and young children. But the neuropathogenic mechanisms of PeV-A3 remain unknown. To understand the pathogenesis of PeV-A3 infection in the neuronal system, PeV-A3-mediated cytopathic effects were analyzed in human glioblastoma cells and neuroblastoma cells. PeV-A3 induced interferons and inflammatory cytokine expression in these neuronal cells. The pronounced cytopathic effects accompanied with activation of death signaling pathways of apoptosis, autophagy, and pyroptosis were detected. A new experimental disease model of parechovirus encephalitis was established. In the disease model, intracranial inoculation with PeV-A3 in C57BL/6 neonatal mice showed body weight loss, hindlimb paralysis, and approximately 20% mortality. PeV-A3 infection in the hippocampus and cortex regions of the neonatal mouse brain was revealed. Mechanistic assay supported the in vitro results, indicating detection of PeV-A3 replication, inflammatory cytokine expression, and death signaling transduction in mouse brain tissues. These in vitro and in vivo studies revealed that the activation of death signaling and inflammation responses is involved in PeV-A3-mediated neurological disorders. The present results might account for some of the PeV-A3-associated clinical manifestations.Ming-Wei JanMing-Wei JanHong-Lin SuTsung-Hsien ChangKuen-Jer TsaiKuen-Jer TsaiFrontiers Media S.A.articleparechovirus A3cytopathic effectinflammationneuronal diseasesinfectious modelImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	parechovirus A3 cytopathic effect inflammation neuronal diseases infectious model Immunologic diseases. Allergy RC581-607
spellingShingle	parechovirus A3 cytopathic effect inflammation neuronal diseases infectious model Immunologic diseases. Allergy RC581-607 Ming-Wei Jan Ming-Wei Jan Hong-Lin Su Tsung-Hsien Chang Kuen-Jer Tsai Kuen-Jer Tsai Characterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus Encephalitis
description	Human parechovirus type 3 (PeV-A3) infection has been recognized as an emerging etiologic factor causing severe nerve disease or sepsis in infants and young children. But the neuropathogenic mechanisms of PeV-A3 remain unknown. To understand the pathogenesis of PeV-A3 infection in the neuronal system, PeV-A3-mediated cytopathic effects were analyzed in human glioblastoma cells and neuroblastoma cells. PeV-A3 induced interferons and inflammatory cytokine expression in these neuronal cells. The pronounced cytopathic effects accompanied with activation of death signaling pathways of apoptosis, autophagy, and pyroptosis were detected. A new experimental disease model of parechovirus encephalitis was established. In the disease model, intracranial inoculation with PeV-A3 in C57BL/6 neonatal mice showed body weight loss, hindlimb paralysis, and approximately 20% mortality. PeV-A3 infection in the hippocampus and cortex regions of the neonatal mouse brain was revealed. Mechanistic assay supported the in vitro results, indicating detection of PeV-A3 replication, inflammatory cytokine expression, and death signaling transduction in mouse brain tissues. These in vitro and in vivo studies revealed that the activation of death signaling and inflammation responses is involved in PeV-A3-mediated neurological disorders. The present results might account for some of the PeV-A3-associated clinical manifestations.
format	article
author	Ming-Wei Jan Ming-Wei Jan Hong-Lin Su Tsung-Hsien Chang Kuen-Jer Tsai Kuen-Jer Tsai
author_facet	Ming-Wei Jan Ming-Wei Jan Hong-Lin Su Tsung-Hsien Chang Kuen-Jer Tsai Kuen-Jer Tsai
author_sort	Ming-Wei Jan
title	Characterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus Encephalitis
title_short	Characterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus Encephalitis
title_full	Characterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus Encephalitis
title_fullStr	Characterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus Encephalitis
title_full_unstemmed	Characterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus Encephalitis
title_sort	characterization of pathogenesis and inflammatory responses to experimental parechovirus encephalitis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2b8c417aad7f48fb99cf8a71a04fcdc7
work_keys_str_mv	AT mingweijan characterizationofpathogenesisandinflammatoryresponsestoexperimentalparechovirusencephalitis AT mingweijan characterizationofpathogenesisandinflammatoryresponsestoexperimentalparechovirusencephalitis AT honglinsu characterizationofpathogenesisandinflammatoryresponsestoexperimentalparechovirusencephalitis AT tsunghsienchang characterizationofpathogenesisandinflammatoryresponsestoexperimentalparechovirusencephalitis AT kuenjertsai characterizationofpathogenesisandinflammatoryresponsestoexperimentalparechovirusencephalitis AT kuenjertsai characterizationofpathogenesisandinflammatoryresponsestoexperimentalparechovirusencephalitis
_version_	1718406251258314752

Characterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus Encephalitis

Ejemplares similares