Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation

Abstract Taste cells undergo constant turnover throughout life; however, the molecular mechanisms governing taste cell generation are not well understood. Using RNA-Seq, we systematically surveyed the transcriptome landscape of taste organoids at different stages of growth. Our data show the staged...

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Autores principales:	Wenwen Ren, Eitaro Aihara, Weiwei Lei, Nishi Gheewala, Hironobu Uchiyama, Robert F. Margolskee, Ken Iwatsuki, Peihua Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b8eaf0b31324533849755c33c546893
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spelling	oai:doaj.org-article:2b8eaf0b31324533849755c33c5468932021-12-02T15:05:50ZTranscriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation10.1038/s41598-017-04099-52045-2322https://doaj.org/article/2b8eaf0b31324533849755c33c5468932017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04099-5https://doaj.org/toc/2045-2322Abstract Taste cells undergo constant turnover throughout life; however, the molecular mechanisms governing taste cell generation are not well understood. Using RNA-Seq, we systematically surveyed the transcriptome landscape of taste organoids at different stages of growth. Our data show the staged expression of a variety of genes and identify multiple signaling pathways underlying taste cell differentiation and taste stem/progenitor cell proliferation. For example, transcripts of taste receptors appear only or predominantly in late-stage organoids. Prior to that, transcription factors and other signaling elements are upregulated. RNA-Seq identified a number of well-characterized signaling pathways in taste organoid cultures, such as those involving Wnt, bone morphogenetic proteins (BMPs), Notch, and Hedgehog (Hh). By pharmacological manipulation, we demonstrate that Wnt, BMPs, Notch, and Hh signaling pathways are necessary for taste cell proliferation, differentiation and cell fate determination. The temporal expression profiles displayed by taste organoids may also lead to the identification of currently unknown transducer elements underlying sour, salt, and other taste qualities, given the staged expression of taste receptor genes and taste transduction elements in cultured organoids.Wenwen RenEitaro AiharaWeiwei LeiNishi GheewalaHironobu UchiyamaRobert F. MargolskeeKen IwatsukiPeihua JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenwen Ren Eitaro Aihara Weiwei Lei Nishi Gheewala Hironobu Uchiyama Robert F. Margolskee Ken Iwatsuki Peihua Jiang Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
description	Abstract Taste cells undergo constant turnover throughout life; however, the molecular mechanisms governing taste cell generation are not well understood. Using RNA-Seq, we systematically surveyed the transcriptome landscape of taste organoids at different stages of growth. Our data show the staged expression of a variety of genes and identify multiple signaling pathways underlying taste cell differentiation and taste stem/progenitor cell proliferation. For example, transcripts of taste receptors appear only or predominantly in late-stage organoids. Prior to that, transcription factors and other signaling elements are upregulated. RNA-Seq identified a number of well-characterized signaling pathways in taste organoid cultures, such as those involving Wnt, bone morphogenetic proteins (BMPs), Notch, and Hedgehog (Hh). By pharmacological manipulation, we demonstrate that Wnt, BMPs, Notch, and Hh signaling pathways are necessary for taste cell proliferation, differentiation and cell fate determination. The temporal expression profiles displayed by taste organoids may also lead to the identification of currently unknown transducer elements underlying sour, salt, and other taste qualities, given the staged expression of taste receptor genes and taste transduction elements in cultured organoids.
format	article
author	Wenwen Ren Eitaro Aihara Weiwei Lei Nishi Gheewala Hironobu Uchiyama Robert F. Margolskee Ken Iwatsuki Peihua Jiang
author_facet	Wenwen Ren Eitaro Aihara Weiwei Lei Nishi Gheewala Hironobu Uchiyama Robert F. Margolskee Ken Iwatsuki Peihua Jiang
author_sort	Wenwen Ren
title	Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title_short	Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title_full	Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title_fullStr	Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title_full_unstemmed	Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title_sort	transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2b8eaf0b31324533849755c33c546893
work_keys_str_mv	AT wenwenren transcriptomeanalysesoftasteorganoidsrevealmultiplepathwaysinvolvedintastecellgeneration AT eitaroaihara transcriptomeanalysesoftasteorganoidsrevealmultiplepathwaysinvolvedintastecellgeneration AT weiweilei transcriptomeanalysesoftasteorganoidsrevealmultiplepathwaysinvolvedintastecellgeneration AT nishigheewala transcriptomeanalysesoftasteorganoidsrevealmultiplepathwaysinvolvedintastecellgeneration AT hironobuuchiyama transcriptomeanalysesoftasteorganoidsrevealmultiplepathwaysinvolvedintastecellgeneration AT robertfmargolskee transcriptomeanalysesoftasteorganoidsrevealmultiplepathwaysinvolvedintastecellgeneration AT keniwatsuki transcriptomeanalysesoftasteorganoidsrevealmultiplepathwaysinvolvedintastecellgeneration AT peihuajiang transcriptomeanalysesoftasteorganoidsrevealmultiplepathwaysinvolvedintastecellgeneration
_version_	1718388694032842752

Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation

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