Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells

Molecular engineering of Cas9 has the potential to expand the application of CRISPR-Cas technology. Here, Ma et al. show that dCas9 can be split and reconstituted in human cells and use a domain swapping strategy to engineer custom Cas9-based logic circuits and sensory switches.

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Detalles Bibliográficos
Autores principales:	Dacheng Ma, Shuguang Peng, Zhen Xie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2b8fed1f7cda4027bcc187fdb24c9236
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spelling	oai:doaj.org-article:2b8fed1f7cda4027bcc187fdb24c92362021-12-02T16:49:57ZIntegration and exchange of split dCas9 domains for transcriptional controls in mammalian cells10.1038/ncomms130562041-1723https://doaj.org/article/2b8fed1f7cda4027bcc187fdb24c92362016-10-01T00:00:00Zhttps://doi.org/10.1038/ncomms13056https://doaj.org/toc/2041-1723Molecular engineering of Cas9 has the potential to expand the application of CRISPR-Cas technology. Here, Ma et al. show that dCas9 can be split and reconstituted in human cells and use a domain swapping strategy to engineer custom Cas9-based logic circuits and sensory switches.Dacheng MaShuguang PengZhen XieNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dacheng Ma Shuguang Peng Zhen Xie Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells
description	Molecular engineering of Cas9 has the potential to expand the application of CRISPR-Cas technology. Here, Ma et al. show that dCas9 can be split and reconstituted in human cells and use a domain swapping strategy to engineer custom Cas9-based logic circuits and sensory switches.
format	article
author	Dacheng Ma Shuguang Peng Zhen Xie
author_facet	Dacheng Ma Shuguang Peng Zhen Xie
author_sort	Dacheng Ma
title	Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells
title_short	Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells
title_full	Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells
title_fullStr	Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells
title_full_unstemmed	Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells
title_sort	integration and exchange of split dcas9 domains for transcriptional controls in mammalian cells
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/2b8fed1f7cda4027bcc187fdb24c9236
work_keys_str_mv	AT dachengma integrationandexchangeofsplitdcas9domainsfortranscriptionalcontrolsinmammaliancells AT shuguangpeng integrationandexchangeofsplitdcas9domainsfortranscriptionalcontrolsinmammaliancells AT zhenxie integrationandexchangeofsplitdcas9domainsfortranscriptionalcontrolsinmammaliancells
_version_	1718383146479648768

Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells

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