LF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway

Abstract Our previous studies showed that low frequency magnetic fields (LF-MF) suppressed tumor growth and influenced the function of immune system. Nevertheless the mechanisms behind the effect of LF-MF still remain to be elucidated. In this study, Tumor- bearing mice subcutaneously inoculated wit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jing Ren, Liang Ding, Qianyun Xu, Guoping Shi, Xiaojing Li, Xiujun Li, Jianjian Ji, Dongya Zhang, Yaping Wang, Tingting Wang, Yayi Hou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b9867850e5845c0bc52c412064a2a68
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2b9867850e5845c0bc52c412064a2a68
record_format	dspace
spelling	oai:doaj.org-article:2b9867850e5845c0bc52c412064a2a682021-12-02T15:18:52ZLF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway10.1038/s41598-017-00913-22045-2322https://doaj.org/article/2b9867850e5845c0bc52c412064a2a682017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00913-2https://doaj.org/toc/2045-2322Abstract Our previous studies showed that low frequency magnetic fields (LF-MF) suppressed tumor growth and influenced the function of immune system. Nevertheless the mechanisms behind the effect of LF-MF still remain to be elucidated. In this study, Tumor- bearing mice subcutaneously inoculated with Lewis lung cancer cells were exposed to a LF-MF (0.4T, 7.5 Hz) for 35 days and Survival rate, tumor growth and the tumor markers were measured. Results showed that tumor growth was obviously inhibited with a prolonged survival of tumor- bearing mice by LF-MF exposure. In vitro experiments, LF-MF was found to induce cell growth arrest, cell senescence and inhibit iron metabolism of lung cancer cells. Moreover, LF-MF stabilized p53 protein via inhibiting cell iron metabolism and the stabilized p53 protein enhanced miR-34a transcription. Furthermore, increased expression of miR-34a induced cell proliferation inhibition, cell cycle arrest and cell senescence of lung cancer cells by targeting E2F1/E2F3. We also detected the relevant indicator in tumor tissue such as the iron content, the level of miR-34a and related protein, corresponding results were obtained. Taken together, these observations imply that LF-MF suppressed lung cancer via inhibiting cell iron metabolism, stabilizing p53 protein and activation P53- miR-34a-E2F1/E2F3 pathway.Jing RenLiang DingQianyun XuGuoping ShiXiaojing LiXiujun LiJianjian JiDongya ZhangYaping WangTingting WangYayi HouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Ren Liang Ding Qianyun Xu Guoping Shi Xiaojing Li Xiujun Li Jianjian Ji Dongya Zhang Yaping Wang Tingting Wang Yayi Hou LF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway
description	Abstract Our previous studies showed that low frequency magnetic fields (LF-MF) suppressed tumor growth and influenced the function of immune system. Nevertheless the mechanisms behind the effect of LF-MF still remain to be elucidated. In this study, Tumor- bearing mice subcutaneously inoculated with Lewis lung cancer cells were exposed to a LF-MF (0.4T, 7.5 Hz) for 35 days and Survival rate, tumor growth and the tumor markers were measured. Results showed that tumor growth was obviously inhibited with a prolonged survival of tumor- bearing mice by LF-MF exposure. In vitro experiments, LF-MF was found to induce cell growth arrest, cell senescence and inhibit iron metabolism of lung cancer cells. Moreover, LF-MF stabilized p53 protein via inhibiting cell iron metabolism and the stabilized p53 protein enhanced miR-34a transcription. Furthermore, increased expression of miR-34a induced cell proliferation inhibition, cell cycle arrest and cell senescence of lung cancer cells by targeting E2F1/E2F3. We also detected the relevant indicator in tumor tissue such as the iron content, the level of miR-34a and related protein, corresponding results were obtained. Taken together, these observations imply that LF-MF suppressed lung cancer via inhibiting cell iron metabolism, stabilizing p53 protein and activation P53- miR-34a-E2F1/E2F3 pathway.
format	article
author	Jing Ren Liang Ding Qianyun Xu Guoping Shi Xiaojing Li Xiujun Li Jianjian Ji Dongya Zhang Yaping Wang Tingting Wang Yayi Hou
author_facet	Jing Ren Liang Ding Qianyun Xu Guoping Shi Xiaojing Li Xiujun Li Jianjian Ji Dongya Zhang Yaping Wang Tingting Wang Yayi Hou
author_sort	Jing Ren
title	LF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway
title_short	LF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway
title_full	LF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway
title_fullStr	LF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway
title_full_unstemmed	LF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway
title_sort	lf-mf inhibits iron metabolism and suppresses lung cancer through activation of p53-mir-34a-e2f1/e2f3 pathway
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2b9867850e5845c0bc52c412064a2a68
work_keys_str_mv	AT jingren lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway AT liangding lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway AT qianyunxu lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway AT guopingshi lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway AT xiaojingli lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway AT xiujunli lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway AT jianjianji lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway AT dongyazhang lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway AT yapingwang lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway AT tingtingwang lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway AT yayihou lfmfinhibitsironmetabolismandsuppresseslungcancerthroughactivationofp53mir34ae2f1e2f3pathway
_version_	1718387438226767872

LF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway

Ejemplares similares