Evolution in quantum leaps: multiple combinatorial transfers of HPI and other genetic modules in Enterobacteriaceae.

Horizontal gene transfer is a key step in the evolution of Enterobacteriaceae. By acquiring virulence determinants of foreign origin, commensals can evolve into pathogens. In Enterobacteriaceae, horizontal transfer of these virulence determinants is largely dependent on transfer by plasmids, phages,...

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Autores principales:	Armand Paauw, Maurine A Leverstein-van Hall, Jan Verhoef, Ad C Fluit
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b99772289ff4752a8f6aa9c0a212bcc
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spelling	oai:doaj.org-article:2b99772289ff4752a8f6aa9c0a212bcc2021-11-25T06:26:45ZEvolution in quantum leaps: multiple combinatorial transfers of HPI and other genetic modules in Enterobacteriaceae.1932-620310.1371/journal.pone.0008662https://doaj.org/article/2b99772289ff4752a8f6aa9c0a212bcc2010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20084283/?tool=EBIhttps://doaj.org/toc/1932-6203Horizontal gene transfer is a key step in the evolution of Enterobacteriaceae. By acquiring virulence determinants of foreign origin, commensals can evolve into pathogens. In Enterobacteriaceae, horizontal transfer of these virulence determinants is largely dependent on transfer by plasmids, phages, genomic islands (GIs) and genomic modules (GMs). The High Pathogenicity Island (HPI) is a GI encoding virulence genes that can be transferred between different Enterobacteriaceae. We investigated the HPI because it was present in an Enterobacter hormaechei outbreak strain (EHOS). Genome sequence analysis showed that the EHOS contained an integration site for mobile elements and harbored two GIs and three putative GMs, including a new variant of the HPI (HPI-ICEEh1). We demonstrate, for the first time, that combinatorial transfers of GIs and GMs between Enterobacter cloacae complex isolates must have occurred. Furthermore, the excision and circularization of several combinations of the GIs and GMs was demonstrated. Because of its flexibility, the multiple integration site of mobile DNA can be considered an integration hotspot (IHS) that increases the genomic plasticity of the bacterium. Multiple combinatorial transfers of diverse combinations of the HPI and other genomic elements among Enterobacteriaceae may accelerate the generation of new pathogenic strains.Armand PaauwMaurine A Leverstein-van HallJan VerhoefAd C FluitPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8662 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Armand Paauw Maurine A Leverstein-van Hall Jan Verhoef Ad C Fluit Evolution in quantum leaps: multiple combinatorial transfers of HPI and other genetic modules in Enterobacteriaceae.
description	Horizontal gene transfer is a key step in the evolution of Enterobacteriaceae. By acquiring virulence determinants of foreign origin, commensals can evolve into pathogens. In Enterobacteriaceae, horizontal transfer of these virulence determinants is largely dependent on transfer by plasmids, phages, genomic islands (GIs) and genomic modules (GMs). The High Pathogenicity Island (HPI) is a GI encoding virulence genes that can be transferred between different Enterobacteriaceae. We investigated the HPI because it was present in an Enterobacter hormaechei outbreak strain (EHOS). Genome sequence analysis showed that the EHOS contained an integration site for mobile elements and harbored two GIs and three putative GMs, including a new variant of the HPI (HPI-ICEEh1). We demonstrate, for the first time, that combinatorial transfers of GIs and GMs between Enterobacter cloacae complex isolates must have occurred. Furthermore, the excision and circularization of several combinations of the GIs and GMs was demonstrated. Because of its flexibility, the multiple integration site of mobile DNA can be considered an integration hotspot (IHS) that increases the genomic plasticity of the bacterium. Multiple combinatorial transfers of diverse combinations of the HPI and other genomic elements among Enterobacteriaceae may accelerate the generation of new pathogenic strains.
format	article
author	Armand Paauw Maurine A Leverstein-van Hall Jan Verhoef Ad C Fluit
author_facet	Armand Paauw Maurine A Leverstein-van Hall Jan Verhoef Ad C Fluit
author_sort	Armand Paauw
title	Evolution in quantum leaps: multiple combinatorial transfers of HPI and other genetic modules in Enterobacteriaceae.
title_short	Evolution in quantum leaps: multiple combinatorial transfers of HPI and other genetic modules in Enterobacteriaceae.
title_full	Evolution in quantum leaps: multiple combinatorial transfers of HPI and other genetic modules in Enterobacteriaceae.
title_fullStr	Evolution in quantum leaps: multiple combinatorial transfers of HPI and other genetic modules in Enterobacteriaceae.
title_full_unstemmed	Evolution in quantum leaps: multiple combinatorial transfers of HPI and other genetic modules in Enterobacteriaceae.
title_sort	evolution in quantum leaps: multiple combinatorial transfers of hpi and other genetic modules in enterobacteriaceae.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/2b99772289ff4752a8f6aa9c0a212bcc
work_keys_str_mv	AT armandpaauw evolutioninquantumleapsmultiplecombinatorialtransfersofhpiandothergeneticmodulesinenterobacteriaceae AT maurinealeversteinvanhall evolutioninquantumleapsmultiplecombinatorialtransfersofhpiandothergeneticmodulesinenterobacteriaceae AT janverhoef evolutioninquantumleapsmultiplecombinatorialtransfersofhpiandothergeneticmodulesinenterobacteriaceae AT adcfluit evolutioninquantumleapsmultiplecombinatorialtransfersofhpiandothergeneticmodulesinenterobacteriaceae
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Evolution in quantum leaps: multiple combinatorial transfers of HPI and other genetic modules in Enterobacteriaceae.

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