Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2

Abstract In our previous work, we reported a novel approach for increasing lipid production in an oleaginous fungus Mucor circinelloides by overexpression of mitochondrial malate transporter protein. This transporter plays a vital role in fatty acid biosynthesis during malate and citrate transport s...

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Autores principales:	Yao Zhang, Qing Liu, Pengcheng Li, Yanxia Wang, Shaoqi Li, Meng Gao, Yuanda Song
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ba01a6b894e48a9a2a19dcddbf07784
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spelling	oai:doaj.org-article:2ba01a6b894e48a9a2a19dcddbf077842021-12-02T16:04:18ZEnhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-210.1038/s41598-021-92324-72045-2322https://doaj.org/article/2ba01a6b894e48a9a2a19dcddbf077842021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92324-7https://doaj.org/toc/2045-2322Abstract In our previous work, we reported a novel approach for increasing lipid production in an oleaginous fungus Mucor circinelloides by overexpression of mitochondrial malate transporter protein. This transporter plays a vital role in fatty acid biosynthesis during malate and citrate transport systems in oleaginous fungi. In this study, the controlling metabolic supplementation strategy was used to improve the lipid production by overexpression of malate transporter protein in M. circinelloides strain coded as Mc-MT-2. The effects of different metabolic intermediates on lipid production in batch fermentation by Mc-MT-2 were investigated. The optimal lipid production was obtained at 0.8% malic acid after 24 h of fermentation. Furthermore, in fed-batch bioreactors containing glucose as a carbon source supplemented with malic acid, the highest cell growth, and lipid production were achieved. The resulting strain showed the fungal dry biomass of 16 g/L, a lipid content of 32%, lipid yield of 5.12 g/L in a controlled bench-top bioreactor, with 1.60-, 1.60- and 2.56-fold improvement, respectively, compared with the batch control without supplementation of malic acid. Our findings revealed that the addition of malic acid during fermentation might play an important role in lipid accumulation in the recombinant M. circinelloides Mc-MT-2. This study provides valuable insights for enhanced microbial lipid production through metabolic supplementation strategy in large scale and industrial applications.Yao ZhangQing LiuPengcheng LiYanxia WangShaoqi LiMeng GaoYuanda SongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yao Zhang Qing Liu Pengcheng Li Yanxia Wang Shaoqi Li Meng Gao Yuanda Song Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2
description	Abstract In our previous work, we reported a novel approach for increasing lipid production in an oleaginous fungus Mucor circinelloides by overexpression of mitochondrial malate transporter protein. This transporter plays a vital role in fatty acid biosynthesis during malate and citrate transport systems in oleaginous fungi. In this study, the controlling metabolic supplementation strategy was used to improve the lipid production by overexpression of malate transporter protein in M. circinelloides strain coded as Mc-MT-2. The effects of different metabolic intermediates on lipid production in batch fermentation by Mc-MT-2 were investigated. The optimal lipid production was obtained at 0.8% malic acid after 24 h of fermentation. Furthermore, in fed-batch bioreactors containing glucose as a carbon source supplemented with malic acid, the highest cell growth, and lipid production were achieved. The resulting strain showed the fungal dry biomass of 16 g/L, a lipid content of 32%, lipid yield of 5.12 g/L in a controlled bench-top bioreactor, with 1.60-, 1.60- and 2.56-fold improvement, respectively, compared with the batch control without supplementation of malic acid. Our findings revealed that the addition of malic acid during fermentation might play an important role in lipid accumulation in the recombinant M. circinelloides Mc-MT-2. This study provides valuable insights for enhanced microbial lipid production through metabolic supplementation strategy in large scale and industrial applications.
format	article
author	Yao Zhang Qing Liu Pengcheng Li Yanxia Wang Shaoqi Li Meng Gao Yuanda Song
author_facet	Yao Zhang Qing Liu Pengcheng Li Yanxia Wang Shaoqi Li Meng Gao Yuanda Song
author_sort	Yao Zhang
title	Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2
title_short	Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2
title_full	Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2
title_fullStr	Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2
title_full_unstemmed	Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2
title_sort	enhanced lipid production by addition of malic acid in fermentation of recombinant mucor circinelloides mc-mt-2
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2ba01a6b894e48a9a2a19dcddbf07784
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_version_	1718385262041497600

Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2

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