Functional regulation of an outer retina hyporeflective band on optical coherence tomography images

Abstract Human and animal retinal optical coherence tomography (OCT) images show a hyporeflective band (HB) between the photoreceptor tip and retinal pigment epithelium layers whose mechanisms are unclear. In mice, HB magnitude and the external limiting membrane-retinal pigment epithelium (ELM-RPE)...

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Autores principales:	Shasha Gao, Yichao Li, David Bissig, Ethan D. Cohen, Robert H. Podolsky, Karen Lins Childers, Gregory Vernon, Sonia Chen, Bruce A. Berkowitz, Haohua Qian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ba6a17ab760416883cef9501d135fa1
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spelling	oai:doaj.org-article:2ba6a17ab760416883cef9501d135fa12021-12-02T15:43:08ZFunctional regulation of an outer retina hyporeflective band on optical coherence tomography images10.1038/s41598-021-89599-12045-2322https://doaj.org/article/2ba6a17ab760416883cef9501d135fa12021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89599-1https://doaj.org/toc/2045-2322Abstract Human and animal retinal optical coherence tomography (OCT) images show a hyporeflective band (HB) between the photoreceptor tip and retinal pigment epithelium layers whose mechanisms are unclear. In mice, HB magnitude and the external limiting membrane-retinal pigment epithelium (ELM-RPE) thickness appear to be dependent on light exposure, which is known to alter photoreceptor mitochondria respiration. Here, we test the hypothesis that these two OCT biomarkers are linked to metabolic activity of the retina. Acetazolamide, which acidifies the subretinal space, had no significant impact on HB magnitude but produced ELM-RPE thinning. Mitochondrial stimulation with 2,4-dinitrophenol reduced both HB magnitude and ELM-RPE thickness in parallel, and also reduced F-actin expression in the same retinal region, but without altering ERG responses. For mice strains with relatively lower (C57BL/6J) or higher (129S6/ev) rod mitochondrial efficacy, light-induced changes in HB magnitude and ELM-RPE thickness were correlated. Humans, analyzed from published data captured with a different protocol, showed a similar light–dark change pattern in HB magnitude as in the mice. Our results indicate that mitochondrial respiration underlies changes in HB magnitude upstream of the pH-sensitive ELM-RPE thickness response. These two distinct OCT biomarkers could be useful indices for non-invasively evaluating photoreceptor mitochondrial metabolic activity.Shasha GaoYichao LiDavid BissigEthan D. CohenRobert H. PodolskyKaren Lins ChildersGregory VernonSonia ChenBruce A. BerkowitzHaohua QianNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shasha Gao Yichao Li David Bissig Ethan D. Cohen Robert H. Podolsky Karen Lins Childers Gregory Vernon Sonia Chen Bruce A. Berkowitz Haohua Qian Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
description	Abstract Human and animal retinal optical coherence tomography (OCT) images show a hyporeflective band (HB) between the photoreceptor tip and retinal pigment epithelium layers whose mechanisms are unclear. In mice, HB magnitude and the external limiting membrane-retinal pigment epithelium (ELM-RPE) thickness appear to be dependent on light exposure, which is known to alter photoreceptor mitochondria respiration. Here, we test the hypothesis that these two OCT biomarkers are linked to metabolic activity of the retina. Acetazolamide, which acidifies the subretinal space, had no significant impact on HB magnitude but produced ELM-RPE thinning. Mitochondrial stimulation with 2,4-dinitrophenol reduced both HB magnitude and ELM-RPE thickness in parallel, and also reduced F-actin expression in the same retinal region, but without altering ERG responses. For mice strains with relatively lower (C57BL/6J) or higher (129S6/ev) rod mitochondrial efficacy, light-induced changes in HB magnitude and ELM-RPE thickness were correlated. Humans, analyzed from published data captured with a different protocol, showed a similar light–dark change pattern in HB magnitude as in the mice. Our results indicate that mitochondrial respiration underlies changes in HB magnitude upstream of the pH-sensitive ELM-RPE thickness response. These two distinct OCT biomarkers could be useful indices for non-invasively evaluating photoreceptor mitochondrial metabolic activity.
format	article
author	Shasha Gao Yichao Li David Bissig Ethan D. Cohen Robert H. Podolsky Karen Lins Childers Gregory Vernon Sonia Chen Bruce A. Berkowitz Haohua Qian
author_facet	Shasha Gao Yichao Li David Bissig Ethan D. Cohen Robert H. Podolsky Karen Lins Childers Gregory Vernon Sonia Chen Bruce A. Berkowitz Haohua Qian
author_sort	Shasha Gao
title	Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title_short	Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title_full	Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title_fullStr	Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title_full_unstemmed	Functional regulation of an outer retina hyporeflective band on optical coherence tomography images
title_sort	functional regulation of an outer retina hyporeflective band on optical coherence tomography images
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2ba6a17ab760416883cef9501d135fa1
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Functional regulation of an outer retina hyporeflective band on optical coherence tomography images

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