Immunochromatographic strip for ultrasensitive detection of fumonisin B1
A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for...
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Taylor & Francis Group
2018
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oai:doaj.org-article:2babf9d852944641afa48a7648b7fd072021-11-26T11:19:46ZImmunochromatographic strip for ultrasensitive detection of fumonisin B10954-01051465-344310.1080/09540105.2018.1439455https://doaj.org/article/2babf9d852944641afa48a7648b7fd072018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1439455https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for FB1 were found to be 50 ng/mL in 0.01 M PBS and 25 ng/mL in corn samples. Results could be obtained within 5 min. The results revealed that the developed method is a rapid, sensitive, and simple tool for the detection of FB1.Kai HaoSteven SuryoprabowoTao HongShanshan SongLiqiang LiuQiankun ZhengHua KuangTaylor & Francis Grouparticlefumonisinsfumonisin b1immunochromatographic assaymonoclonal antibodystrip testAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 699-710 (2018) |
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DOAJ |
language |
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topic |
fumonisins fumonisin b1 immunochromatographic assay monoclonal antibody strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 |
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fumonisins fumonisin b1 immunochromatographic assay monoclonal antibody strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Kai Hao Steven Suryoprabowo Tao Hong Shanshan Song Liqiang Liu Qiankun Zheng Hua Kuang Immunochromatographic strip for ultrasensitive detection of fumonisin B1 |
description |
A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for FB1 were found to be 50 ng/mL in 0.01 M PBS and 25 ng/mL in corn samples. Results could be obtained within 5 min. The results revealed that the developed method is a rapid, sensitive, and simple tool for the detection of FB1. |
format |
article |
author |
Kai Hao Steven Suryoprabowo Tao Hong Shanshan Song Liqiang Liu Qiankun Zheng Hua Kuang |
author_facet |
Kai Hao Steven Suryoprabowo Tao Hong Shanshan Song Liqiang Liu Qiankun Zheng Hua Kuang |
author_sort |
Kai Hao |
title |
Immunochromatographic strip for ultrasensitive detection of fumonisin B1 |
title_short |
Immunochromatographic strip for ultrasensitive detection of fumonisin B1 |
title_full |
Immunochromatographic strip for ultrasensitive detection of fumonisin B1 |
title_fullStr |
Immunochromatographic strip for ultrasensitive detection of fumonisin B1 |
title_full_unstemmed |
Immunochromatographic strip for ultrasensitive detection of fumonisin B1 |
title_sort |
immunochromatographic strip for ultrasensitive detection of fumonisin b1 |
publisher |
Taylor & Francis Group |
publishDate |
2018 |
url |
https://doaj.org/article/2babf9d852944641afa48a7648b7fd07 |
work_keys_str_mv |
AT kaihao immunochromatographicstripforultrasensitivedetectionoffumonisinb1 AT stevensuryoprabowo immunochromatographicstripforultrasensitivedetectionoffumonisinb1 AT taohong immunochromatographicstripforultrasensitivedetectionoffumonisinb1 AT shanshansong immunochromatographicstripforultrasensitivedetectionoffumonisinb1 AT liqiangliu immunochromatographicstripforultrasensitivedetectionoffumonisinb1 AT qiankunzheng immunochromatographicstripforultrasensitivedetectionoffumonisinb1 AT huakuang immunochromatographicstripforultrasensitivedetectionoffumonisinb1 |
_version_ |
1718409524772077568 |