Immunochromatographic strip for ultrasensitive detection of fumonisin B1

A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for...

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Autores principales: Kai Hao, Steven Suryoprabowo, Tao Hong, Shanshan Song, Liqiang Liu, Qiankun Zheng, Hua Kuang
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Lenguaje:EN
Publicado: Taylor & Francis Group 2018
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Acceso en línea:https://doaj.org/article/2babf9d852944641afa48a7648b7fd07
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spelling oai:doaj.org-article:2babf9d852944641afa48a7648b7fd072021-11-26T11:19:46ZImmunochromatographic strip for ultrasensitive detection of fumonisin B10954-01051465-344310.1080/09540105.2018.1439455https://doaj.org/article/2babf9d852944641afa48a7648b7fd072018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1439455https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for FB1 were found to be 50 ng/mL in 0.01 M PBS and 25 ng/mL in corn samples. Results could be obtained within 5 min. The results revealed that the developed method is a rapid, sensitive, and simple tool for the detection of FB1.Kai HaoSteven SuryoprabowoTao HongShanshan SongLiqiang LiuQiankun ZhengHua KuangTaylor & Francis Grouparticlefumonisinsfumonisin b1immunochromatographic assaymonoclonal antibodystrip testAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 699-710 (2018)
institution DOAJ
collection DOAJ
language EN
topic fumonisins
fumonisin b1
immunochromatographic assay
monoclonal antibody
strip test
Agriculture (General)
S1-972
Immunologic diseases. Allergy
RC581-607
spellingShingle fumonisins
fumonisin b1
immunochromatographic assay
monoclonal antibody
strip test
Agriculture (General)
S1-972
Immunologic diseases. Allergy
RC581-607
Kai Hao
Steven Suryoprabowo
Tao Hong
Shanshan Song
Liqiang Liu
Qiankun Zheng
Hua Kuang
Immunochromatographic strip for ultrasensitive detection of fumonisin B1
description A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for FB1 were found to be 50 ng/mL in 0.01 M PBS and 25 ng/mL in corn samples. Results could be obtained within 5 min. The results revealed that the developed method is a rapid, sensitive, and simple tool for the detection of FB1.
format article
author Kai Hao
Steven Suryoprabowo
Tao Hong
Shanshan Song
Liqiang Liu
Qiankun Zheng
Hua Kuang
author_facet Kai Hao
Steven Suryoprabowo
Tao Hong
Shanshan Song
Liqiang Liu
Qiankun Zheng
Hua Kuang
author_sort Kai Hao
title Immunochromatographic strip for ultrasensitive detection of fumonisin B1
title_short Immunochromatographic strip for ultrasensitive detection of fumonisin B1
title_full Immunochromatographic strip for ultrasensitive detection of fumonisin B1
title_fullStr Immunochromatographic strip for ultrasensitive detection of fumonisin B1
title_full_unstemmed Immunochromatographic strip for ultrasensitive detection of fumonisin B1
title_sort immunochromatographic strip for ultrasensitive detection of fumonisin b1
publisher Taylor & Francis Group
publishDate 2018
url https://doaj.org/article/2babf9d852944641afa48a7648b7fd07
work_keys_str_mv AT kaihao immunochromatographicstripforultrasensitivedetectionoffumonisinb1
AT stevensuryoprabowo immunochromatographicstripforultrasensitivedetectionoffumonisinb1
AT taohong immunochromatographicstripforultrasensitivedetectionoffumonisinb1
AT shanshansong immunochromatographicstripforultrasensitivedetectionoffumonisinb1
AT liqiangliu immunochromatographicstripforultrasensitivedetectionoffumonisinb1
AT qiankunzheng immunochromatographicstripforultrasensitivedetectionoffumonisinb1
AT huakuang immunochromatographicstripforultrasensitivedetectionoffumonisinb1
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