Pitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits

Abstract The spleen is a hematopoietic organ that participates in cellular and humoral immunity. It also serves as a quality control mechanism for removing senescent and/or poorly deformable red blood cells (RBCs) from circulation. Pitting is a specialized process by which the spleen extracts partic...

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Autores principales:	Aleix Elizalde-Torrent, Claudia Trejo-Soto, Lourdes Méndez-Mora, Marc Nicolau, Oihane Ezama, Melisa Gualdrón-López, Carmen Fernández-Becerra, Tomás Alarcón, Aurora Hernández-Machado, Hernando A. del Portillo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2bac6c7ddd0f45178048f1465ce3fb33
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spelling	oai:doaj.org-article:2bac6c7ddd0f45178048f1465ce3fb332021-11-14T12:19:50ZPitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits10.1038/s41598-021-01568-w2045-2322https://doaj.org/article/2bac6c7ddd0f45178048f1465ce3fb332021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01568-whttps://doaj.org/toc/2045-2322Abstract The spleen is a hematopoietic organ that participates in cellular and humoral immunity. It also serves as a quality control mechanism for removing senescent and/or poorly deformable red blood cells (RBCs) from circulation. Pitting is a specialized process by which the spleen extracts particles, including malaria parasites, from within circulating RBCs during their passage through the interendothelial slits (IES) in the splenic cords. To study this physiological function in vitro, we have developed two microfluidic devices modeling the IES, according to the hypothesis that at a certain range of mechanical stress on the RBC, regulated through both slit size and blood flow, would force it undergo the pitting process without affecting the cell integrity. To prove its functionality in replicating pitting of malaria parasites, we have performed a characterization of P. falciparum-infected RBCs (P.f.-RBCs) after their passage through the devices, determining hemolysis and the proportion of once-infected RBCs (O-iRBCs), defined by the presence of a parasite antigen and absence of DAPI staining of parasite DNA using a flow cytometry-based approach. The passage of P.f.-RBCs through the devices at the physiological flow rate did not affect cell integrity and resulted in an increase of the frequency of O-iRBCs. Both microfluidic device models were capable to replicate the pitting of P.f.-RBCs ex vivo by means of mechanical constraints without cellular involvement, shedding new insights on the role of the spleen in the pathophysiology of malaria.Aleix Elizalde-TorrentClaudia Trejo-SotoLourdes Méndez-MoraMarc NicolauOihane EzamaMelisa Gualdrón-LópezCarmen Fernández-BecerraTomás AlarcónAurora Hernández-MachadoHernando A. del PortilloNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aleix Elizalde-Torrent Claudia Trejo-Soto Lourdes Méndez-Mora Marc Nicolau Oihane Ezama Melisa Gualdrón-López Carmen Fernández-Becerra Tomás Alarcón Aurora Hernández-Machado Hernando A. del Portillo Pitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits
description	Abstract The spleen is a hematopoietic organ that participates in cellular and humoral immunity. It also serves as a quality control mechanism for removing senescent and/or poorly deformable red blood cells (RBCs) from circulation. Pitting is a specialized process by which the spleen extracts particles, including malaria parasites, from within circulating RBCs during their passage through the interendothelial slits (IES) in the splenic cords. To study this physiological function in vitro, we have developed two microfluidic devices modeling the IES, according to the hypothesis that at a certain range of mechanical stress on the RBC, regulated through both slit size and blood flow, would force it undergo the pitting process without affecting the cell integrity. To prove its functionality in replicating pitting of malaria parasites, we have performed a characterization of P. falciparum-infected RBCs (P.f.-RBCs) after their passage through the devices, determining hemolysis and the proportion of once-infected RBCs (O-iRBCs), defined by the presence of a parasite antigen and absence of DAPI staining of parasite DNA using a flow cytometry-based approach. The passage of P.f.-RBCs through the devices at the physiological flow rate did not affect cell integrity and resulted in an increase of the frequency of O-iRBCs. Both microfluidic device models were capable to replicate the pitting of P.f.-RBCs ex vivo by means of mechanical constraints without cellular involvement, shedding new insights on the role of the spleen in the pathophysiology of malaria.
format	article
author	Aleix Elizalde-Torrent Claudia Trejo-Soto Lourdes Méndez-Mora Marc Nicolau Oihane Ezama Melisa Gualdrón-López Carmen Fernández-Becerra Tomás Alarcón Aurora Hernández-Machado Hernando A. del Portillo
author_facet	Aleix Elizalde-Torrent Claudia Trejo-Soto Lourdes Méndez-Mora Marc Nicolau Oihane Ezama Melisa Gualdrón-López Carmen Fernández-Becerra Tomás Alarcón Aurora Hernández-Machado Hernando A. del Portillo
author_sort	Aleix Elizalde-Torrent
title	Pitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits
title_short	Pitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits
title_full	Pitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits
title_fullStr	Pitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits
title_full_unstemmed	Pitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits
title_sort	pitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2bac6c7ddd0f45178048f1465ce3fb33
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Pitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits

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