The possible association of clusterin fucosylation changes with male fertility disorders

Abstract In the seminal plasma (n = 118) and serum (n = 90) clusterin (CLU) the fucosylation and the expression of selected fucosyltransferases (FUTs) were analyzed. Samples from infertile men were divided into groups based on the results of the standard semen analysis: normozoospermic (N), teratozo...

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Autores principales:	Ewa Janiszewska, Izabela Kokot, Iwona Gilowska, Ricardo Faundez, Ewa Maria Kratz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2bb4aba6a1234fefb90cbfa20877bc7c
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spelling	oai:doaj.org-article:2bb4aba6a1234fefb90cbfa20877bc7c2021-12-02T17:06:33ZThe possible association of clusterin fucosylation changes with male fertility disorders10.1038/s41598-021-95288-w2045-2322https://doaj.org/article/2bb4aba6a1234fefb90cbfa20877bc7c2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95288-whttps://doaj.org/toc/2045-2322Abstract In the seminal plasma (n = 118) and serum (n = 90) clusterin (CLU) the fucosylation and the expression of selected fucosyltransferases (FUTs) were analyzed. Samples from infertile men were divided into groups based on the results of the standard semen analysis: normozoospermic (N), teratozoospermic (T), asthenoteratozoospermic (AT) and oligoasthenoteratozoospermic (OAT). The CLU fucosylation was analyzed using lectin-ELISAs with biotinylated lectins specific to α1,3-, α1,2-linked antennary fucose, and α1,6-linked core fucose (LTA, UEA, and LCA, respectively). The concentrations of FUT3 and FUT4, reflecting the expression of Le oligosaccharide structures, were measured using ELISA tests. The differences in serum CLU and FUT4 concentrations, and in the expression of core fucose and antennary fucose α1,2-linked in CLU glycans between the N group and other groups examined suggest that the disturbances in sperm count, motility, and morphology are not the only cause of male infertility. Lack of similarities between levels of examined parameters in blood serum and seminal plasma may suggest the differences in mechanisms leading to glycoproteins glycosylation. It confirmed the observed differences in concentrations of seminal plasma CLU, FUT3, and FUT4 between the OAT group and N, T, AT groups, indicating that decreased sperm count may be related to these parameters expression. The serum CLU concentrations and expression of core fucose and fucose α1,2-linked in CLU, seem to be good markers differentiating normozoospermic men from those with abnormal sperm parameters, which was not observed for seminal plasma.Ewa JaniszewskaIzabela KokotIwona GilowskaRicardo FaundezEwa Maria KratzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ewa Janiszewska Izabela Kokot Iwona Gilowska Ricardo Faundez Ewa Maria Kratz The possible association of clusterin fucosylation changes with male fertility disorders
description	Abstract In the seminal plasma (n = 118) and serum (n = 90) clusterin (CLU) the fucosylation and the expression of selected fucosyltransferases (FUTs) were analyzed. Samples from infertile men were divided into groups based on the results of the standard semen analysis: normozoospermic (N), teratozoospermic (T), asthenoteratozoospermic (AT) and oligoasthenoteratozoospermic (OAT). The CLU fucosylation was analyzed using lectin-ELISAs with biotinylated lectins specific to α1,3-, α1,2-linked antennary fucose, and α1,6-linked core fucose (LTA, UEA, and LCA, respectively). The concentrations of FUT3 and FUT4, reflecting the expression of Le oligosaccharide structures, were measured using ELISA tests. The differences in serum CLU and FUT4 concentrations, and in the expression of core fucose and antennary fucose α1,2-linked in CLU glycans between the N group and other groups examined suggest that the disturbances in sperm count, motility, and morphology are not the only cause of male infertility. Lack of similarities between levels of examined parameters in blood serum and seminal plasma may suggest the differences in mechanisms leading to glycoproteins glycosylation. It confirmed the observed differences in concentrations of seminal plasma CLU, FUT3, and FUT4 between the OAT group and N, T, AT groups, indicating that decreased sperm count may be related to these parameters expression. The serum CLU concentrations and expression of core fucose and fucose α1,2-linked in CLU, seem to be good markers differentiating normozoospermic men from those with abnormal sperm parameters, which was not observed for seminal plasma.
format	article
author	Ewa Janiszewska Izabela Kokot Iwona Gilowska Ricardo Faundez Ewa Maria Kratz
author_facet	Ewa Janiszewska Izabela Kokot Iwona Gilowska Ricardo Faundez Ewa Maria Kratz
author_sort	Ewa Janiszewska
title	The possible association of clusterin fucosylation changes with male fertility disorders
title_short	The possible association of clusterin fucosylation changes with male fertility disorders
title_full	The possible association of clusterin fucosylation changes with male fertility disorders
title_fullStr	The possible association of clusterin fucosylation changes with male fertility disorders
title_full_unstemmed	The possible association of clusterin fucosylation changes with male fertility disorders
title_sort	possible association of clusterin fucosylation changes with male fertility disorders
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2bb4aba6a1234fefb90cbfa20877bc7c
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The possible association of clusterin fucosylation changes with male fertility disorders

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