Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper

Abstract Heavy metal sequestration from industrial wastes and agricultural soils is a long-standing challenge. This is more critical for copper since copper pollution is hazardous both for the environment and for human health. In this study, we applied an integrated approach of Darwin’s theory of na...

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Autores principales:	Dharmender K. Gahlot, Nayyer Taheri, Dhani Ram Mahato, Matthew S. Francis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2bbbd0f170a34f1193bd6e8c999d53b3
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spelling	oai:doaj.org-article:2bbbd0f170a34f1193bd6e8c999d53b32021-12-02T12:33:45ZBioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper10.1038/s41598-020-76178-z2045-2322https://doaj.org/article/2bbbd0f170a34f1193bd6e8c999d53b32020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-76178-zhttps://doaj.org/toc/2045-2322Abstract Heavy metal sequestration from industrial wastes and agricultural soils is a long-standing challenge. This is more critical for copper since copper pollution is hazardous both for the environment and for human health. In this study, we applied an integrated approach of Darwin’s theory of natural selection with bacterial genetic engineering to generate a biological system with an application for the accumulation of Cu2+ ions. A library of recombinant non-pathogenic Escherichia coli strains was engineered to express seven potential Cu2+ binding peptides encoded by a ‘synthetic degenerate’ DNA motif and fused to Maltose Binding Protein (MBP). Most of these peptide-MBP chimeras conferred tolerance to high concentrations of copper sulphate, and in certain cases in the order of 160-fold higher than the recognised EC50 toxic levels of copper in soils. UV–Vis spectroscopic analysis indicated a molar ratio of peptide-copper complexes, while a combination of bioinformatics-based structure modelling, Cu2+ ion docking, and MD simulations of peptide-MBP chimeras corroborated the extent of Cu2+ binding among the peptides. Further, in silico analysis predicted the peptides possessed binding affinity toward a broad range of divalent metal ions. Thus, we report on an efficient, cost-effective, and environment-friendly prototype biological system that is potentially capable of copper bioaccumulation, and which could easily be adapted for the removal of other hazardous heavy metals or the bio-mining of rare metals.Dharmender K. GahlotNayyer TaheriDhani Ram MahatoMatthew S. FrancisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dharmender K. Gahlot Nayyer Taheri Dhani Ram Mahato Matthew S. Francis Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper
description	Abstract Heavy metal sequestration from industrial wastes and agricultural soils is a long-standing challenge. This is more critical for copper since copper pollution is hazardous both for the environment and for human health. In this study, we applied an integrated approach of Darwin’s theory of natural selection with bacterial genetic engineering to generate a biological system with an application for the accumulation of Cu2+ ions. A library of recombinant non-pathogenic Escherichia coli strains was engineered to express seven potential Cu2+ binding peptides encoded by a ‘synthetic degenerate’ DNA motif and fused to Maltose Binding Protein (MBP). Most of these peptide-MBP chimeras conferred tolerance to high concentrations of copper sulphate, and in certain cases in the order of 160-fold higher than the recognised EC50 toxic levels of copper in soils. UV–Vis spectroscopic analysis indicated a molar ratio of peptide-copper complexes, while a combination of bioinformatics-based structure modelling, Cu2+ ion docking, and MD simulations of peptide-MBP chimeras corroborated the extent of Cu2+ binding among the peptides. Further, in silico analysis predicted the peptides possessed binding affinity toward a broad range of divalent metal ions. Thus, we report on an efficient, cost-effective, and environment-friendly prototype biological system that is potentially capable of copper bioaccumulation, and which could easily be adapted for the removal of other hazardous heavy metals or the bio-mining of rare metals.
format	article
author	Dharmender K. Gahlot Nayyer Taheri Dhani Ram Mahato Matthew S. Francis
author_facet	Dharmender K. Gahlot Nayyer Taheri Dhani Ram Mahato Matthew S. Francis
author_sort	Dharmender K. Gahlot
title	Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper
title_short	Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper
title_full	Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper
title_fullStr	Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper
title_full_unstemmed	Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper
title_sort	bioengineering of non-pathogenic escherichia coli to enrich for accumulation of environmental copper
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2bbbd0f170a34f1193bd6e8c999d53b3
work_keys_str_mv	AT dharmenderkgahlot bioengineeringofnonpathogenicescherichiacolitoenrichforaccumulationofenvironmentalcopper AT nayyertaheri bioengineeringofnonpathogenicescherichiacolitoenrichforaccumulationofenvironmentalcopper AT dhanirammahato bioengineeringofnonpathogenicescherichiacolitoenrichforaccumulationofenvironmentalcopper AT matthewsfrancis bioengineeringofnonpathogenicescherichiacolitoenrichforaccumulationofenvironmentalcopper
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Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper

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