Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber
Cashmere fineness is one of the important factors determining cashmere quality; however, our understanding of the regulation of cashmere fineness at the cellular level is limited. Here, we used single-cell RNA sequencing and computational models to identify 13 skin cell types in Liaoning cashmere go...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:2bbf72de8d3c4fefbe44a2c7254949d92021-11-10T08:01:44ZSingle-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber1664-802110.3389/fgene.2021.726670https://doaj.org/article/2bbf72de8d3c4fefbe44a2c7254949d92021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.726670/fullhttps://doaj.org/toc/1664-8021Cashmere fineness is one of the important factors determining cashmere quality; however, our understanding of the regulation of cashmere fineness at the cellular level is limited. Here, we used single-cell RNA sequencing and computational models to identify 13 skin cell types in Liaoning cashmere goats. We also analyzed the molecular changes in the development process by cell trajectory analysis and revealed the maturation process in the gene expression profile in Liaoning cashmere goats. Weighted gene co-expression network analysis explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2, a marker gene of SDPCs, was selected for immunofluorescence (IF) and Western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results show different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify and prove the differential expression in the coarse and fine types of Liaoning cashmere goats. Therefore, the CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat SDPCs; our research provides new insights into the mechanism of cashmere growth and fineness regulation by cells.Zeying WangZeying WangYanru WangTaiyu HuiRui ChenYanan XuYu ZhangHe TianWei WangYuyan CongSuping GuoYanxu ZhuXinghui ZhangDan GuoMan BaiYixing FanChang YueZhixian BaiJiaming SunWeidong CaiXinjiang ZhangMing GuYuting QinYinggang SunYanzhi WuRina WuXingtang DouWenlin BaiYuanyuan ZhengFrontiers Media S.A.article10 × Genomics single-cell sequencingcashmere finenessSDPCsCOL1A1ACTA2CXCL8GeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
10 × Genomics single-cell sequencing cashmere fineness SDPCs COL1A1 ACTA2 CXCL8 Genetics QH426-470 |
| spellingShingle |
10 × Genomics single-cell sequencing cashmere fineness SDPCs COL1A1 ACTA2 CXCL8 Genetics QH426-470 Zeying Wang Zeying Wang Yanru Wang Taiyu Hui Rui Chen Yanan Xu Yu Zhang He Tian Wei Wang Yuyan Cong Suping Guo Yanxu Zhu Xinghui Zhang Dan Guo Man Bai Yixing Fan Chang Yue Zhixian Bai Jiaming Sun Weidong Cai Xinjiang Zhang Ming Gu Yuting Qin Yinggang Sun Yanzhi Wu Rina Wu Xingtang Dou Wenlin Bai Yuanyuan Zheng Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber |
| description |
Cashmere fineness is one of the important factors determining cashmere quality; however, our understanding of the regulation of cashmere fineness at the cellular level is limited. Here, we used single-cell RNA sequencing and computational models to identify 13 skin cell types in Liaoning cashmere goats. We also analyzed the molecular changes in the development process by cell trajectory analysis and revealed the maturation process in the gene expression profile in Liaoning cashmere goats. Weighted gene co-expression network analysis explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2, a marker gene of SDPCs, was selected for immunofluorescence (IF) and Western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results show different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify and prove the differential expression in the coarse and fine types of Liaoning cashmere goats. Therefore, the CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat SDPCs; our research provides new insights into the mechanism of cashmere growth and fineness regulation by cells. |
| format |
article |
| author |
Zeying Wang Zeying Wang Yanru Wang Taiyu Hui Rui Chen Yanan Xu Yu Zhang He Tian Wei Wang Yuyan Cong Suping Guo Yanxu Zhu Xinghui Zhang Dan Guo Man Bai Yixing Fan Chang Yue Zhixian Bai Jiaming Sun Weidong Cai Xinjiang Zhang Ming Gu Yuting Qin Yinggang Sun Yanzhi Wu Rina Wu Xingtang Dou Wenlin Bai Yuanyuan Zheng |
| author_facet |
Zeying Wang Zeying Wang Yanru Wang Taiyu Hui Rui Chen Yanan Xu Yu Zhang He Tian Wei Wang Yuyan Cong Suping Guo Yanxu Zhu Xinghui Zhang Dan Guo Man Bai Yixing Fan Chang Yue Zhixian Bai Jiaming Sun Weidong Cai Xinjiang Zhang Ming Gu Yuting Qin Yinggang Sun Yanzhi Wu Rina Wu Xingtang Dou Wenlin Bai Yuanyuan Zheng |
| author_sort |
Zeying Wang |
| title |
Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber |
| title_short |
Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber |
| title_full |
Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber |
| title_fullStr |
Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber |
| title_full_unstemmed |
Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber |
| title_sort |
single-cell sequencing reveals differential cell types in skin tissues of liaoning cashmere goats and key genes related potentially to the fineness of cashmere fiber |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/2bbf72de8d3c4fefbe44a2c7254949d9 |
| work_keys_str_mv |
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