Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces

Xi Yang,1,2 Liping Zhu,1 Seiichi Tada,1 Di Zhou,3 Takashi Kitajima,1 Takashi Isoshima,1 Yasuhiro Yoshida,1,4 Mariko Nakamura,1,5 Weiqun Yan,2 Yoshihiro Ito1,3 1Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan; 2School of Pharmaceutical Sciences, Jilin University, Jilin, People’s...

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Autores principales:	Yang X, Zhu L, Tada S, Zhou D, Kitajima T, Isoshima T, Yoshida Y, Nakamura M, Yan W, Ito Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2bc1e63872e74ae491ef2947c20ee8e7
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spelling	oai:doaj.org-article:2bc1e63872e74ae491ef2947c20ee8e72021-12-02T03:05:17ZMussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces1178-2013https://doaj.org/article/2bc1e63872e74ae491ef2947c20ee8e72014-05-01T00:00:00Zhttp://www.dovepress.com/mussel-inspired-human-gelatin-nanocoating-for-creating-biologically-ad-a17055https://doaj.org/toc/1178-2013 Xi Yang,1,2 Liping Zhu,1 Seiichi Tada,1 Di Zhou,3 Takashi Kitajima,1 Takashi Isoshima,1 Yasuhiro Yoshida,1,4 Mariko Nakamura,1,5 Weiqun Yan,2 Yoshihiro Ito1,3 1Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan; 2School of Pharmaceutical Sciences, Jilin University, Jilin, People’s Republic of China; 3Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, Saitama, 4Department of Biomaterials and Bioengineering, Graduate School of Dental Medicine, Hokkaido University, Hokkaido, 5Dental Hygiene Program, Kibi International College, Okayama, Japan Abstract: Recombinant human gelatin was conjugated with dopamine using carbodiimide as a surface modifier. This dopamine-coupled human gelatin (D-rhG) was characterized by 1H-nuclear magnetic resonance, mass spectroscopy, and circular dichroism. D-rhG-coated surface properties were analyzed by physicochemical methods. Additionally, cell attachment and growth on the modified surfaces was assessed using human umbilical endothelial cells. Binding of gelatin onto titanium was significantly enhanced by dopamine conjugation. The thickness of the D-rhG coating depended on the treatment pH; thicker layers were formed at higher pH values, with a maximum thickness of 30 nm. D-rhG enhanced the binding of collagen-binding vascular endothelial growth factor and cell adhesion as compared with gelatin alone, even at the same surface concentration. The D-rhG surface modifier enhanced substrate binding by creating an adhesive nanointerface that increased specific protein binding and cell attachment. Keywords: recombinant human gelatin, dopamine, natural catechols, cell adhesion, cell culture, titaniumYang XZhu LTada SZhou DKitajima TIsoshima TYoshida YNakamura MYan WIto YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2753-2765 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yang X Zhu L Tada S Zhou D Kitajima T Isoshima T Yoshida Y Nakamura M Yan W Ito Y Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces
description	Xi Yang,1,2 Liping Zhu,1 Seiichi Tada,1 Di Zhou,3 Takashi Kitajima,1 Takashi Isoshima,1 Yasuhiro Yoshida,1,4 Mariko Nakamura,1,5 Weiqun Yan,2 Yoshihiro Ito1,3 1Nano Medical Engineering Laboratory, RIKEN, Saitama, Japan; 2School of Pharmaceutical Sciences, Jilin University, Jilin, People’s Republic of China; 3Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, Saitama, 4Department of Biomaterials and Bioengineering, Graduate School of Dental Medicine, Hokkaido University, Hokkaido, 5Dental Hygiene Program, Kibi International College, Okayama, Japan Abstract: Recombinant human gelatin was conjugated with dopamine using carbodiimide as a surface modifier. This dopamine-coupled human gelatin (D-rhG) was characterized by 1H-nuclear magnetic resonance, mass spectroscopy, and circular dichroism. D-rhG-coated surface properties were analyzed by physicochemical methods. Additionally, cell attachment and growth on the modified surfaces was assessed using human umbilical endothelial cells. Binding of gelatin onto titanium was significantly enhanced by dopamine conjugation. The thickness of the D-rhG coating depended on the treatment pH; thicker layers were formed at higher pH values, with a maximum thickness of 30 nm. D-rhG enhanced the binding of collagen-binding vascular endothelial growth factor and cell adhesion as compared with gelatin alone, even at the same surface concentration. The D-rhG surface modifier enhanced substrate binding by creating an adhesive nanointerface that increased specific protein binding and cell attachment. Keywords: recombinant human gelatin, dopamine, natural catechols, cell adhesion, cell culture, titanium
format	article
author	Yang X Zhu L Tada S Zhou D Kitajima T Isoshima T Yoshida Y Nakamura M Yan W Ito Y
author_facet	Yang X Zhu L Tada S Zhou D Kitajima T Isoshima T Yoshida Y Nakamura M Yan W Ito Y
author_sort	Yang X
title	Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces
title_short	Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces
title_full	Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces
title_fullStr	Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces
title_full_unstemmed	Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces
title_sort	mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/2bc1e63872e74ae491ef2947c20ee8e7
work_keys_str_mv	AT yangx musselinspiredhumangelatinnanocoatingforcreatingbiologicallyadhesivesurfaces AT zhul musselinspiredhumangelatinnanocoatingforcreatingbiologicallyadhesivesurfaces AT tadas musselinspiredhumangelatinnanocoatingforcreatingbiologicallyadhesivesurfaces AT zhoud musselinspiredhumangelatinnanocoatingforcreatingbiologicallyadhesivesurfaces AT kitajimat musselinspiredhumangelatinnanocoatingforcreatingbiologicallyadhesivesurfaces AT isoshimat musselinspiredhumangelatinnanocoatingforcreatingbiologicallyadhesivesurfaces AT yoshiday musselinspiredhumangelatinnanocoatingforcreatingbiologicallyadhesivesurfaces AT nakamuram musselinspiredhumangelatinnanocoatingforcreatingbiologicallyadhesivesurfaces AT yanw musselinspiredhumangelatinnanocoatingforcreatingbiologicallyadhesivesurfaces AT itoy musselinspiredhumangelatinnanocoatingforcreatingbiologicallyadhesivesurfaces
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Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces

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