Self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential

Compared with primary and local tumor, metastasis is more difficult to resect completely and remain the leading cause of death associated with solid tumors. It is therefore pressing to develop effective strategies to prevent and suppress tumor metastasis in its early stages. Heparanase overexpressio...

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Autores principales:	Xiqi Ma, Xiaojuan Wang, Cai Liu, Baosheng Ge, Hua He, Qi Dai, Zhixiong Zhang, Jinyi Yu, Werner M. Nau, Fang Huang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Delivery system Gold nanoclusters Self-assembly Fluorescence antisense microRNA Materials of engineering and construction. Mechanics of materials TA401-492
Acceso en línea:	https://doaj.org/article/2bc4ca922cf64f269c4bfcbda6b6db4f
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spelling	oai:doaj.org-article:2bc4ca922cf64f269c4bfcbda6b6db4f2021-11-04T04:25:59ZSelf-assembled theranostic microcarrier targeting tumor cells with high metastatic potential0264-127510.1016/j.matdes.2021.110196https://doaj.org/article/2bc4ca922cf64f269c4bfcbda6b6db4f2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0264127521007516https://doaj.org/toc/0264-1275Compared with primary and local tumor, metastasis is more difficult to resect completely and remain the leading cause of death associated with solid tumors. It is therefore pressing to develop effective strategies to prevent and suppress tumor metastasis in its early stages. Heparanase overexpression is significantly associated with increased malignancy and metastasis. Here, we develop a novel heparanase degradable heparin-based microcarrier, HBMA, for specific staining, targeting, and inhibiting tumor cells with high metastatic potential. HBMA is fabricated through self-assembling of three components, positively charged fluorescent gold nanoclusters (KG-AuNCs), heparin polymers (HP), and antisense miRNA-21 oligonucleotides (AM-21), with each component playing multiple roles. Results of confocal microscopy and flow cytometry show that this agent has an excellent capability to label metastatic tumor cells with high selectivity. On the therapeutic side, the results of cell viability assay, wound healing assay, and plate colony formation assay verify that HBMA induces a significant inhibition effect towards the proliferation and migration of the selectively target tumor cells. The heparanase responsive AM-21 delivery, the specific staining, and the efficient tumor cell activity suppression highlight HBMA as a promising cancer theranostic agent to prevent tumor metastasis.Xiqi MaXiaojuan WangCai LiuBaosheng GeHua HeQi DaiZhixiong ZhangJinyi YuWerner M. NauFang HuangElsevierarticleDelivery systemGold nanoclustersSelf-assemblyFluorescenceantisense microRNAMaterials of engineering and construction. Mechanics of materialsTA401-492ENMaterials & Design, Vol 212, Iss , Pp 110196- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Delivery system Gold nanoclusters Self-assembly Fluorescence antisense microRNA Materials of engineering and construction. Mechanics of materials TA401-492
spellingShingle	Delivery system Gold nanoclusters Self-assembly Fluorescence antisense microRNA Materials of engineering and construction. Mechanics of materials TA401-492 Xiqi Ma Xiaojuan Wang Cai Liu Baosheng Ge Hua He Qi Dai Zhixiong Zhang Jinyi Yu Werner M. Nau Fang Huang Self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential
description	Compared with primary and local tumor, metastasis is more difficult to resect completely and remain the leading cause of death associated with solid tumors. It is therefore pressing to develop effective strategies to prevent and suppress tumor metastasis in its early stages. Heparanase overexpression is significantly associated with increased malignancy and metastasis. Here, we develop a novel heparanase degradable heparin-based microcarrier, HBMA, for specific staining, targeting, and inhibiting tumor cells with high metastatic potential. HBMA is fabricated through self-assembling of three components, positively charged fluorescent gold nanoclusters (KG-AuNCs), heparin polymers (HP), and antisense miRNA-21 oligonucleotides (AM-21), with each component playing multiple roles. Results of confocal microscopy and flow cytometry show that this agent has an excellent capability to label metastatic tumor cells with high selectivity. On the therapeutic side, the results of cell viability assay, wound healing assay, and plate colony formation assay verify that HBMA induces a significant inhibition effect towards the proliferation and migration of the selectively target tumor cells. The heparanase responsive AM-21 delivery, the specific staining, and the efficient tumor cell activity suppression highlight HBMA as a promising cancer theranostic agent to prevent tumor metastasis.
format	article
author	Xiqi Ma Xiaojuan Wang Cai Liu Baosheng Ge Hua He Qi Dai Zhixiong Zhang Jinyi Yu Werner M. Nau Fang Huang
author_facet	Xiqi Ma Xiaojuan Wang Cai Liu Baosheng Ge Hua He Qi Dai Zhixiong Zhang Jinyi Yu Werner M. Nau Fang Huang
author_sort	Xiqi Ma
title	Self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential
title_short	Self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential
title_full	Self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential
title_fullStr	Self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential
title_full_unstemmed	Self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential
title_sort	self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2bc4ca922cf64f269c4bfcbda6b6db4f
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Self-assembled theranostic microcarrier targeting tumor cells with high metastatic potential

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