Comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes.

Rice is sensitive to chilling stress, especially at the seedling stage. To elucidate the molecular genetic mechanisms of chilling tolerance in rice, comprehensive gene expressions of two rice genotypes (chilling-tolerant LTH and chilling-sensitive IR29) with contrasting responses to chilling stress...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ting Zhang, Xiuqin Zhao, Wensheng Wang, Yajiao Pan, Liyu Huang, Xiaoyue Liu, Ying Zong, Linghua Zhu, Daichang Yang, Binying Fu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2bc4d523ddb04e429241e39ac2bd89ea
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2bc4d523ddb04e429241e39ac2bd89ea
record_format	dspace
spelling	oai:doaj.org-article:2bc4d523ddb04e429241e39ac2bd89ea2021-11-18T07:08:24ZComparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes.1932-620310.1371/journal.pone.0043274https://doaj.org/article/2bc4d523ddb04e429241e39ac2bd89ea2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22912843/?tool=EBIhttps://doaj.org/toc/1932-6203Rice is sensitive to chilling stress, especially at the seedling stage. To elucidate the molecular genetic mechanisms of chilling tolerance in rice, comprehensive gene expressions of two rice genotypes (chilling-tolerant LTH and chilling-sensitive IR29) with contrasting responses to chilling stress were comparatively analyzed. Results revealed a differential constitutive gene expression prior to stress and distinct global transcription reprogramming between the two rice genotypes under time-series chilling stress and subsequent recovery conditions. A set of genes with higher basal expression were identified in chilling-tolerant LTH compared with chilling-sensitive IR29, indicating their possible role in intrinsic tolerance to chilling stress. Under chilling stress, the major effect on gene expression was up-regulation in the chilling- tolerant genotype and strong repression in chilling-sensitive genotype. Early responses to chilling stress in both genotypes featured commonly up-regulated genes related to transcription regulation and signal transduction, while functional categories for late phase chilling regulated genes were diverse with a wide range of functional adaptations to continuous stress. Following the cessation of chilling treatments, there was quick and efficient reversion of gene expression in the chilling-tolerant genotype, while the chilling-sensitive genotype displayed considerably slower recovering capacity at the transcriptional level. In addition, the detection of differentially-regulated TF genes and enriched cis-elements demonstrated that multiple regulatory pathways, including CBF and MYBS3 regulons, were involved in chilling stress tolerance. A number of the chilling-regulated genes identified in this study were co-localized onto previously fine-mapped cold-tolerance-related QTLs, providing candidates for gene cloning and elucidation of molecular mechanisms responsible for chilling tolerance in rice.Ting ZhangXiuqin ZhaoWensheng WangYajiao PanLiyu HuangXiaoyue LiuYing ZongLinghua ZhuDaichang YangBinying FuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43274 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ting Zhang Xiuqin Zhao Wensheng Wang Yajiao Pan Liyu Huang Xiaoyue Liu Ying Zong Linghua Zhu Daichang Yang Binying Fu Comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes.
description	Rice is sensitive to chilling stress, especially at the seedling stage. To elucidate the molecular genetic mechanisms of chilling tolerance in rice, comprehensive gene expressions of two rice genotypes (chilling-tolerant LTH and chilling-sensitive IR29) with contrasting responses to chilling stress were comparatively analyzed. Results revealed a differential constitutive gene expression prior to stress and distinct global transcription reprogramming between the two rice genotypes under time-series chilling stress and subsequent recovery conditions. A set of genes with higher basal expression were identified in chilling-tolerant LTH compared with chilling-sensitive IR29, indicating their possible role in intrinsic tolerance to chilling stress. Under chilling stress, the major effect on gene expression was up-regulation in the chilling- tolerant genotype and strong repression in chilling-sensitive genotype. Early responses to chilling stress in both genotypes featured commonly up-regulated genes related to transcription regulation and signal transduction, while functional categories for late phase chilling regulated genes were diverse with a wide range of functional adaptations to continuous stress. Following the cessation of chilling treatments, there was quick and efficient reversion of gene expression in the chilling-tolerant genotype, while the chilling-sensitive genotype displayed considerably slower recovering capacity at the transcriptional level. In addition, the detection of differentially-regulated TF genes and enriched cis-elements demonstrated that multiple regulatory pathways, including CBF and MYBS3 regulons, were involved in chilling stress tolerance. A number of the chilling-regulated genes identified in this study were co-localized onto previously fine-mapped cold-tolerance-related QTLs, providing candidates for gene cloning and elucidation of molecular mechanisms responsible for chilling tolerance in rice.
format	article
author	Ting Zhang Xiuqin Zhao Wensheng Wang Yajiao Pan Liyu Huang Xiaoyue Liu Ying Zong Linghua Zhu Daichang Yang Binying Fu
author_facet	Ting Zhang Xiuqin Zhao Wensheng Wang Yajiao Pan Liyu Huang Xiaoyue Liu Ying Zong Linghua Zhu Daichang Yang Binying Fu
author_sort	Ting Zhang
title	Comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes.
title_short	Comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes.
title_full	Comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes.
title_fullStr	Comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes.
title_full_unstemmed	Comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes.
title_sort	comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2bc4d523ddb04e429241e39ac2bd89ea
work_keys_str_mv	AT tingzhang comparativetranscriptomeprofilingofchillingstressresponsivenessintwocontrastingricegenotypes AT xiuqinzhao comparativetranscriptomeprofilingofchillingstressresponsivenessintwocontrastingricegenotypes AT wenshengwang comparativetranscriptomeprofilingofchillingstressresponsivenessintwocontrastingricegenotypes AT yajiaopan comparativetranscriptomeprofilingofchillingstressresponsivenessintwocontrastingricegenotypes AT liyuhuang comparativetranscriptomeprofilingofchillingstressresponsivenessintwocontrastingricegenotypes AT xiaoyueliu comparativetranscriptomeprofilingofchillingstressresponsivenessintwocontrastingricegenotypes AT yingzong comparativetranscriptomeprofilingofchillingstressresponsivenessintwocontrastingricegenotypes AT linghuazhu comparativetranscriptomeprofilingofchillingstressresponsivenessintwocontrastingricegenotypes AT daichangyang comparativetranscriptomeprofilingofchillingstressresponsivenessintwocontrastingricegenotypes AT binyingfu comparativetranscriptomeprofilingofchillingstressresponsivenessintwocontrastingricegenotypes
_version_	1718423842410463232

Comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes.

Ejemplares similares