B1-CELLS OF INNATE IMMUNITY IN THE BONE MARROW IN BREAST CANCER PATIENTS: IDENTIFICATION AND THEIR RELATIONSHIP WITH CLINICAL AND MORPHOLOGICAL PARAMETERS

Objective: In recent years more attention has been paid to the study of the innate immune system, which includes B1-lymphocytes. They produce pentameric M antibodies, which play an important role in the induction of apoptosis in tumor cells. The study of lymphocyte populations can help to reveal the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Svetlana Chulkova, Chulkova Tupitsyn
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Diseases of the blood and blood-forming organs RC633-647.5
Acceso en línea:	https://doaj.org/article/2bc5a71ec27c4462a6896f9d9504f5ff
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2bc5a71ec27c4462a6896f9d9504f5ff
record_format	dspace
spelling	oai:doaj.org-article:2bc5a71ec27c4462a6896f9d9504f5ff2021-11-10T04:33:02ZB1-CELLS OF INNATE IMMUNITY IN THE BONE MARROW IN BREAST CANCER PATIENTS: IDENTIFICATION AND THEIR RELATIONSHIP WITH CLINICAL AND MORPHOLOGICAL PARAMETERS2531-137910.1016/j.htct.2021.10.982https://doaj.org/article/2bc5a71ec27c4462a6896f9d9504f5ff2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2531137921011299https://doaj.org/toc/2531-1379Objective: In recent years more attention has been paid to the study of the innate immune system, which includes B1-lymphocytes. They produce pentameric M antibodies, which play an important role in the induction of apoptosis in tumor cells. The study of lymphocyte populations can help to reveal the phenomenon of persistence of disseminated tumor cells in the bone marrow (BM) of breast cancer (BC) patients. Methodology: This study included BM punctuates from 64 BC patients and 10 women with benign processes. The study was carried out by two methods: morphological and immunological. Calculation of the myelogram under light microscopy was performed by two expert morphologists. Multiparameter flow cytometry (FACSCanto II cytometer) has been used to assess the populations of BM lymphocytes. Antibodies CD20, CD5, CD19, CD38, CD22, CD45 were used. Results: The content of B1 (CD5+) cells is higher in luminal B-Her2 “+” BC, than with B-Her2 “-”: 10.2% (n=10) versus 4.0% (n=20), p=0.032. The highest levels of B1-cells were observed in stage IIA (12.4±10.7%), also with 2 affected lymph nodes and their maximum size: 16.0±10.2% (n=5) and 5.8±1.6% (n=29), p=0.07. The content of B1-cells correlated with eosinophilic myelocytes (R=0.365; p=0.011; n=48), plasma cells (R=0.409; p=0.004; n=48) in BC. Conclusion: The determination of the level of B1-lymphocytes in the BM can serve as an additional marker of the molecular subtype of BC. It is described that an increase in the content of plasma cells takes place with DTC in the bone marrow. Based on this it can be assumed an increase in the level of B1-lymphocytes is associated with a high probability of metastases in the BM.Svetlana ChulkovaChulkova TupitsynElsevierarticleDiseases of the blood and blood-forming organsRC633-647.5ENHematology, Transfusion and Cell Therapy, Vol 43, Iss , Pp S21- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diseases of the blood and blood-forming organs RC633-647.5
spellingShingle	Diseases of the blood and blood-forming organs RC633-647.5 Svetlana Chulkova Chulkova Tupitsyn B1-CELLS OF INNATE IMMUNITY IN THE BONE MARROW IN BREAST CANCER PATIENTS: IDENTIFICATION AND THEIR RELATIONSHIP WITH CLINICAL AND MORPHOLOGICAL PARAMETERS
description	Objective: In recent years more attention has been paid to the study of the innate immune system, which includes B1-lymphocytes. They produce pentameric M antibodies, which play an important role in the induction of apoptosis in tumor cells. The study of lymphocyte populations can help to reveal the phenomenon of persistence of disseminated tumor cells in the bone marrow (BM) of breast cancer (BC) patients. Methodology: This study included BM punctuates from 64 BC patients and 10 women with benign processes. The study was carried out by two methods: morphological and immunological. Calculation of the myelogram under light microscopy was performed by two expert morphologists. Multiparameter flow cytometry (FACSCanto II cytometer) has been used to assess the populations of BM lymphocytes. Antibodies CD20, CD5, CD19, CD38, CD22, CD45 were used. Results: The content of B1 (CD5+) cells is higher in luminal B-Her2 “+” BC, than with B-Her2 “-”: 10.2% (n=10) versus 4.0% (n=20), p=0.032. The highest levels of B1-cells were observed in stage IIA (12.4±10.7%), also with 2 affected lymph nodes and their maximum size: 16.0±10.2% (n=5) and 5.8±1.6% (n=29), p=0.07. The content of B1-cells correlated with eosinophilic myelocytes (R=0.365; p=0.011; n=48), plasma cells (R=0.409; p=0.004; n=48) in BC. Conclusion: The determination of the level of B1-lymphocytes in the BM can serve as an additional marker of the molecular subtype of BC. It is described that an increase in the content of plasma cells takes place with DTC in the bone marrow. Based on this it can be assumed an increase in the level of B1-lymphocytes is associated with a high probability of metastases in the BM.
format	article
author	Svetlana Chulkova Chulkova Tupitsyn
author_facet	Svetlana Chulkova Chulkova Tupitsyn
author_sort	Svetlana Chulkova
title	B1-CELLS OF INNATE IMMUNITY IN THE BONE MARROW IN BREAST CANCER PATIENTS: IDENTIFICATION AND THEIR RELATIONSHIP WITH CLINICAL AND MORPHOLOGICAL PARAMETERS
title_short	B1-CELLS OF INNATE IMMUNITY IN THE BONE MARROW IN BREAST CANCER PATIENTS: IDENTIFICATION AND THEIR RELATIONSHIP WITH CLINICAL AND MORPHOLOGICAL PARAMETERS
title_full	B1-CELLS OF INNATE IMMUNITY IN THE BONE MARROW IN BREAST CANCER PATIENTS: IDENTIFICATION AND THEIR RELATIONSHIP WITH CLINICAL AND MORPHOLOGICAL PARAMETERS
title_fullStr	B1-CELLS OF INNATE IMMUNITY IN THE BONE MARROW IN BREAST CANCER PATIENTS: IDENTIFICATION AND THEIR RELATIONSHIP WITH CLINICAL AND MORPHOLOGICAL PARAMETERS
title_full_unstemmed	B1-CELLS OF INNATE IMMUNITY IN THE BONE MARROW IN BREAST CANCER PATIENTS: IDENTIFICATION AND THEIR RELATIONSHIP WITH CLINICAL AND MORPHOLOGICAL PARAMETERS
title_sort	b1-cells of innate immunity in the bone marrow in breast cancer patients: identification and their relationship with clinical and morphological parameters
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2bc5a71ec27c4462a6896f9d9504f5ff
work_keys_str_mv	AT svetlanachulkova b1cellsofinnateimmunityinthebonemarrowinbreastcancerpatientsidentificationandtheirrelationshipwithclinicalandmorphologicalparameters AT chulkovatupitsyn b1cellsofinnateimmunityinthebonemarrowinbreastcancerpatientsidentificationandtheirrelationshipwithclinicalandmorphologicalparameters
_version_	1718440634696597504

B1-CELLS OF INNATE IMMUNITY IN THE BONE MARROW IN BREAST CANCER PATIENTS: IDENTIFICATION AND THEIR RELATIONSHIP WITH CLINICAL AND MORPHOLOGICAL PARAMETERS

Ejemplares similares