Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in <i>Dasypyrum villosum</i> L. Reveals That <i>DvGELP53</i> Is Related to BSMV Infection

GDSL-type esterase/lipase proteins (GELPs) characterized by a conserved GDSL motif at their N-terminus belong to the lipid hydrolysis enzyme superfamily. In plants, GELPs play an important role in plant growth, development and stress response. The studies of the identification and characterization o...

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Autores principales:	Heng Zhang, Xu Zhang, Jia Zhao, Li Sun, Haiyan Wang, Ying Zhu, Jin Xiao, Xiue Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	GELP gene family <i>Dasypyrum villosum</i> L. virus movement Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2bcb5ef2335d469c9bf358ad9c218675
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spelling	oai:doaj.org-article:2bcb5ef2335d469c9bf358ad9c2186752021-11-25T17:55:22ZGenome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in <i>Dasypyrum villosum</i> L. Reveals That <i>DvGELP53</i> Is Related to BSMV Infection10.3390/ijms2222123171422-00671661-6596https://doaj.org/article/2bcb5ef2335d469c9bf358ad9c2186752021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12317https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067GDSL-type esterase/lipase proteins (GELPs) characterized by a conserved GDSL motif at their N-terminus belong to the lipid hydrolysis enzyme superfamily. In plants, GELPs play an important role in plant growth, development and stress response. The studies of the identification and characterization of the <i>GELP</i> gene family in Triticeae have not been reported. In this study, 193 <i>DvGELPs</i> were identified in <i>Dasypyrum villosum</i> and classified into 11 groups (clade A–K) by means of phylogenetic analysis. Most <i>DvGELPs</i> contain only one GDSL domain, only four <i>DvGELPs</i> contain other domains besides the GDSL domain. Gene structure analysis indicated 35.2% <i>DvGELP</i> genes have four introns and five exons. In the promoter regions of the identified <i>DvGELP</i><i>s</i>, we detected 4502 putative <i>cis</i>-elements, which were associated with plant hormones, plant growth, environmental stress and light responsiveness. Expression profiling revealed 36, 44 and 17 <i>DvGELPs</i> were highly expressed in the spike, the root and the grain, respectively. Further investigation of a root-specific expressing <i>GELP</i>, <i>DvGELP53</i>, indicated it was induced by a variety of biotic and abiotic stresses. The knockdown of <i>DvGELP53</i> inhibited long-distance movement of BSMV in the tissue of <i>D. villosum</i>. This research provides a genome-wide glimpse of the <i>D. villosum GELP</i> genes and hints at the participation of <i>DvGELP53</i> in the interaction between virus and plants.Heng ZhangXu ZhangJia ZhaoLi SunHaiyan WangYing ZhuJin XiaoXiue WangMDPI AGarticleGELPgene family<i>Dasypyrum villosum</i> L.virus movementBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12317, p 12317 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	GELP gene family <i>Dasypyrum villosum</i> L. virus movement Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	GELP gene family <i>Dasypyrum villosum</i> L. virus movement Biology (General) QH301-705.5 Chemistry QD1-999 Heng Zhang Xu Zhang Jia Zhao Li Sun Haiyan Wang Ying Zhu Jin Xiao Xiue Wang Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in <i>Dasypyrum villosum</i> L. Reveals That <i>DvGELP53</i> Is Related to BSMV Infection
description	GDSL-type esterase/lipase proteins (GELPs) characterized by a conserved GDSL motif at their N-terminus belong to the lipid hydrolysis enzyme superfamily. In plants, GELPs play an important role in plant growth, development and stress response. The studies of the identification and characterization of the <i>GELP</i> gene family in Triticeae have not been reported. In this study, 193 <i>DvGELPs</i> were identified in <i>Dasypyrum villosum</i> and classified into 11 groups (clade A–K) by means of phylogenetic analysis. Most <i>DvGELPs</i> contain only one GDSL domain, only four <i>DvGELPs</i> contain other domains besides the GDSL domain. Gene structure analysis indicated 35.2% <i>DvGELP</i> genes have four introns and five exons. In the promoter regions of the identified <i>DvGELP</i><i>s</i>, we detected 4502 putative <i>cis</i>-elements, which were associated with plant hormones, plant growth, environmental stress and light responsiveness. Expression profiling revealed 36, 44 and 17 <i>DvGELPs</i> were highly expressed in the spike, the root and the grain, respectively. Further investigation of a root-specific expressing <i>GELP</i>, <i>DvGELP53</i>, indicated it was induced by a variety of biotic and abiotic stresses. The knockdown of <i>DvGELP53</i> inhibited long-distance movement of BSMV in the tissue of <i>D. villosum</i>. This research provides a genome-wide glimpse of the <i>D. villosum GELP</i> genes and hints at the participation of <i>DvGELP53</i> in the interaction between virus and plants.
format	article
author	Heng Zhang Xu Zhang Jia Zhao Li Sun Haiyan Wang Ying Zhu Jin Xiao Xiue Wang
author_facet	Heng Zhang Xu Zhang Jia Zhao Li Sun Haiyan Wang Ying Zhu Jin Xiao Xiue Wang
author_sort	Heng Zhang
title	Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in <i>Dasypyrum villosum</i> L. Reveals That <i>DvGELP53</i> Is Related to BSMV Infection
title_short	Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in <i>Dasypyrum villosum</i> L. Reveals That <i>DvGELP53</i> Is Related to BSMV Infection
title_full	Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in <i>Dasypyrum villosum</i> L. Reveals That <i>DvGELP53</i> Is Related to BSMV Infection
title_fullStr	Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in <i>Dasypyrum villosum</i> L. Reveals That <i>DvGELP53</i> Is Related to BSMV Infection
title_full_unstemmed	Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in <i>Dasypyrum villosum</i> L. Reveals That <i>DvGELP53</i> Is Related to BSMV Infection
title_sort	genome-wide identification of gdsl-type esterase/lipase gene family in <i>dasypyrum villosum</i> l. reveals that <i>dvgelp53</i> is related to bsmv infection
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2bcb5ef2335d469c9bf358ad9c218675
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Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in <i>Dasypyrum villosum</i> L. Reveals That <i>DvGELP53</i> Is Related to BSMV Infection

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