Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.

Infrapatellar fat pad adipose stem cells (IPFP-ASCs) have been shown to harbor chondrogenic potential. When combined with 3D polymeric structures, the stem cells provide a source of stem cells to engineer 3D tissues for cartilage repair. In this study, we have shown human IPFP-ASCs seeded onto 3D pr...

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Autores principales:	Ken Ye, Raed Felimban, Kathy Traianedes, Simon E Moulton, Gordon G Wallace, Johnson Chung, Anita Quigley, Peter F M Choong, Damian E Myers
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2bd7def61ef24639a20c76c7e52f3f2f
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spelling	oai:doaj.org-article:2bd7def61ef24639a20c76c7e52f3f2f2021-11-18T08:16:04ZChondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.1932-620310.1371/journal.pone.0099410https://doaj.org/article/2bd7def61ef24639a20c76c7e52f3f2f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24918443/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Infrapatellar fat pad adipose stem cells (IPFP-ASCs) have been shown to harbor chondrogenic potential. When combined with 3D polymeric structures, the stem cells provide a source of stem cells to engineer 3D tissues for cartilage repair. In this study, we have shown human IPFP-ASCs seeded onto 3D printed chitosan scaffolds can undergo chondrogenesis using TGFβ3 and BMP6. By week 4, a pearlescent, cartilage-like matrix had formed that penetrated the top layers of the chitosan scaffold forming a 'cap' on the scaffold. Chondrocytic morphology showed typical cells encased in extracellular matrix which stained positively with toluidine blue. Immunohistochemistry demonstrated positive staining for collagen type II and cartilage proteoglycans, as well as collagen type I. Real time PCR analysis showed up-regulation of collagen type II, aggrecan and SOX9 genes when IPFP-ASCs were stimulated by TGFβ3 and BMP6. Thus, IPFP-ASCs can successfully undergo chondrogenesis using TGFβ3 and BMP6 and the cartilage-like tissue that forms on the surface of 3D-printed chitosan scaffold may prove useful as an osteochondral graft.Ken YeRaed FelimbanKathy TraianedesSimon E MoultonGordon G WallaceJohnson ChungAnita QuigleyPeter F M ChoongPeter F M ChoongDamian E MyersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99410 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ken Ye Raed Felimban Kathy Traianedes Simon E Moulton Gordon G Wallace Johnson Chung Anita Quigley Peter F M Choong Peter F M Choong Damian E Myers Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.
description	Infrapatellar fat pad adipose stem cells (IPFP-ASCs) have been shown to harbor chondrogenic potential. When combined with 3D polymeric structures, the stem cells provide a source of stem cells to engineer 3D tissues for cartilage repair. In this study, we have shown human IPFP-ASCs seeded onto 3D printed chitosan scaffolds can undergo chondrogenesis using TGFβ3 and BMP6. By week 4, a pearlescent, cartilage-like matrix had formed that penetrated the top layers of the chitosan scaffold forming a 'cap' on the scaffold. Chondrocytic morphology showed typical cells encased in extracellular matrix which stained positively with toluidine blue. Immunohistochemistry demonstrated positive staining for collagen type II and cartilage proteoglycans, as well as collagen type I. Real time PCR analysis showed up-regulation of collagen type II, aggrecan and SOX9 genes when IPFP-ASCs were stimulated by TGFβ3 and BMP6. Thus, IPFP-ASCs can successfully undergo chondrogenesis using TGFβ3 and BMP6 and the cartilage-like tissue that forms on the surface of 3D-printed chitosan scaffold may prove useful as an osteochondral graft.
format	article
author	Ken Ye Raed Felimban Kathy Traianedes Simon E Moulton Gordon G Wallace Johnson Chung Anita Quigley Peter F M Choong Peter F M Choong Damian E Myers
author_facet	Ken Ye Raed Felimban Kathy Traianedes Simon E Moulton Gordon G Wallace Johnson Chung Anita Quigley Peter F M Choong Peter F M Choong Damian E Myers
author_sort	Ken Ye
title	Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.
title_short	Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.
title_full	Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.
title_fullStr	Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.
title_full_unstemmed	Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.
title_sort	chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3d printed chitosan scaffold.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2bd7def61ef24639a20c76c7e52f3f2f
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Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.

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