Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2

Fatty acid overload, either of the saturated palmitic acid (PA) or the unsaturated oleic acid (OA), causes triglyceride accumulation into specialized organelles termed lipid droplets (LD). However, only PA overload leads to liver damage mediated by mitochondrial dysfunction. Whether these divergent...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andrea Eynaudi, Francisco Díaz-Castro, Juan Carlos Bórquez, Roberto Bravo-Sagua, Valentina Parra, Rodrigo Troncoso
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lipid droplets mitochondria fatty acids hepatocytes oxygen consumption Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/2bd959abe94c4e5cbbb90ed1d6c7ed4b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2bd959abe94c4e5cbbb90ed1d6c7ed4b
record_format	dspace
spelling	oai:doaj.org-article:2bd959abe94c4e5cbbb90ed1d6c7ed4b2021-11-12T04:51:27ZDifferential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG22296-861X10.3389/fnut.2021.775382https://doaj.org/article/2bd959abe94c4e5cbbb90ed1d6c7ed4b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnut.2021.775382/fullhttps://doaj.org/toc/2296-861XFatty acid overload, either of the saturated palmitic acid (PA) or the unsaturated oleic acid (OA), causes triglyceride accumulation into specialized organelles termed lipid droplets (LD). However, only PA overload leads to liver damage mediated by mitochondrial dysfunction. Whether these divergent outcomes stem from differential effects of PA and OA on LD and mitochondria joint dynamics remains to be uncovered. Here, we contrast how both fatty acids impact the morphology and interaction between both organelles and mitochondrial bioenergetics in HepG2 cells. Using confocal microscopy, we showed that short-term (2–24 h) OA overload promotes more and bigger LD accumulation than PA. Oxygen polarography indicated that both treatments stimulated mitochondrial respiration; however, OA favored an overall build-up of the mitochondrial potential, and PA evoked mitochondrial fragmentation, concomitant with an ATP-oriented metabolism. Even though PA-induced a lesser increase in LD-mitochondria proximity than OA, those LD associated with highly active mitochondria suggest that they interact mainly to fuel fatty acid oxidation and ATP synthesis (that is, metabolically “active” LD). On the contrary, OA overload seemingly stimulated LD-mitochondria interaction mainly for LD growth (thus metabolically “passive” LDs). In sum, these differences point out that OA readily accumulates in LD, likely reducing their toxicity, while PA preferably stimulates mitochondrial oxidative metabolism, which may contribute to liver damage progression.Andrea EynaudiFrancisco Díaz-CastroJuan Carlos BórquezRoberto Bravo-SaguaRoberto Bravo-SaguaRoberto Bravo-SaguaValentina ParraValentina ParraRodrigo TroncosoRodrigo TroncosoFrontiers Media S.A.articlelipid dropletsmitochondriafatty acidshepatocytesoxygen consumptionNutrition. Foods and food supplyTX341-641ENFrontiers in Nutrition, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipid droplets mitochondria fatty acids hepatocytes oxygen consumption Nutrition. Foods and food supply TX341-641
spellingShingle	lipid droplets mitochondria fatty acids hepatocytes oxygen consumption Nutrition. Foods and food supply TX341-641 Andrea Eynaudi Francisco Díaz-Castro Juan Carlos Bórquez Roberto Bravo-Sagua Roberto Bravo-Sagua Roberto Bravo-Sagua Valentina Parra Valentina Parra Rodrigo Troncoso Rodrigo Troncoso Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2
description	Fatty acid overload, either of the saturated palmitic acid (PA) or the unsaturated oleic acid (OA), causes triglyceride accumulation into specialized organelles termed lipid droplets (LD). However, only PA overload leads to liver damage mediated by mitochondrial dysfunction. Whether these divergent outcomes stem from differential effects of PA and OA on LD and mitochondria joint dynamics remains to be uncovered. Here, we contrast how both fatty acids impact the morphology and interaction between both organelles and mitochondrial bioenergetics in HepG2 cells. Using confocal microscopy, we showed that short-term (2–24 h) OA overload promotes more and bigger LD accumulation than PA. Oxygen polarography indicated that both treatments stimulated mitochondrial respiration; however, OA favored an overall build-up of the mitochondrial potential, and PA evoked mitochondrial fragmentation, concomitant with an ATP-oriented metabolism. Even though PA-induced a lesser increase in LD-mitochondria proximity than OA, those LD associated with highly active mitochondria suggest that they interact mainly to fuel fatty acid oxidation and ATP synthesis (that is, metabolically “active” LD). On the contrary, OA overload seemingly stimulated LD-mitochondria interaction mainly for LD growth (thus metabolically “passive” LDs). In sum, these differences point out that OA readily accumulates in LD, likely reducing their toxicity, while PA preferably stimulates mitochondrial oxidative metabolism, which may contribute to liver damage progression.
format	article
author	Andrea Eynaudi Francisco Díaz-Castro Juan Carlos Bórquez Roberto Bravo-Sagua Roberto Bravo-Sagua Roberto Bravo-Sagua Valentina Parra Valentina Parra Rodrigo Troncoso Rodrigo Troncoso
author_facet	Andrea Eynaudi Francisco Díaz-Castro Juan Carlos Bórquez Roberto Bravo-Sagua Roberto Bravo-Sagua Roberto Bravo-Sagua Valentina Parra Valentina Parra Rodrigo Troncoso Rodrigo Troncoso
author_sort	Andrea Eynaudi
title	Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2
title_short	Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2
title_full	Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2
title_fullStr	Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2
title_full_unstemmed	Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2
title_sort	differential effects of oleic and palmitic acids on lipid droplet-mitochondria interaction in the hepatic cell line hepg2
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2bd959abe94c4e5cbbb90ed1d6c7ed4b
work_keys_str_mv	AT andreaeynaudi differentialeffectsofoleicandpalmiticacidsonlipiddropletmitochondriainteractioninthehepaticcelllinehepg2 AT franciscodiazcastro differentialeffectsofoleicandpalmiticacidsonlipiddropletmitochondriainteractioninthehepaticcelllinehepg2 AT juancarlosborquez differentialeffectsofoleicandpalmiticacidsonlipiddropletmitochondriainteractioninthehepaticcelllinehepg2 AT robertobravosagua differentialeffectsofoleicandpalmiticacidsonlipiddropletmitochondriainteractioninthehepaticcelllinehepg2 AT robertobravosagua differentialeffectsofoleicandpalmiticacidsonlipiddropletmitochondriainteractioninthehepaticcelllinehepg2 AT robertobravosagua differentialeffectsofoleicandpalmiticacidsonlipiddropletmitochondriainteractioninthehepaticcelllinehepg2 AT valentinaparra differentialeffectsofoleicandpalmiticacidsonlipiddropletmitochondriainteractioninthehepaticcelllinehepg2 AT valentinaparra differentialeffectsofoleicandpalmiticacidsonlipiddropletmitochondriainteractioninthehepaticcelllinehepg2 AT rodrigotroncoso differentialeffectsofoleicandpalmiticacidsonlipiddropletmitochondriainteractioninthehepaticcelllinehepg2 AT rodrigotroncoso differentialeffectsofoleicandpalmiticacidsonlipiddropletmitochondriainteractioninthehepaticcelllinehepg2
_version_	1718431159858233344

Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2

Ejemplares similares