egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development

Epidermal growth factor-like domain multiple 6 (Egfl6) is a basement membrane protein and plays an important role in hair follicle morphogenesis, angiogenesis, notochord development in vertebrates. Although egfl6 expression in the developing head was observed in zebrafish, its role for craniofacial...

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Autores principales:	Sil Jin, Hyejee Na, Haewon Jeon, Jangwon Park, Chong Pyo Choe
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	egfl6 pharyngeal pouch craniofacial development zebrafish Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2be1e7bc76c042deae4f25fc11fc5dae
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spelling	oai:doaj.org-article:2be1e7bc76c042deae4f25fc11fc5dae2021-11-04T15:00:42Zegfl6 expression in the pharyngeal pouch is dispensable for craniofacial development1976-83542151-248510.1080/19768354.2021.1970018https://doaj.org/article/2be1e7bc76c042deae4f25fc11fc5dae2021-09-01T00:00:00Zhttp://dx.doi.org/10.1080/19768354.2021.1970018https://doaj.org/toc/1976-8354https://doaj.org/toc/2151-2485Epidermal growth factor-like domain multiple 6 (Egfl6) is a basement membrane protein and plays an important role in hair follicle morphogenesis, angiogenesis, notochord development in vertebrates. Although egfl6 expression in the developing head was observed in zebrafish, its role for craniofacial development and the determination of the pharyngeal region expressing egfl6, have not been reported yet. Here, we report the expression patterns and function of egfl6 in craniofacial development in zebrafish. egfl6 was expressed sequentially in the developing pharyngeal pouches that are key epithelial structures governing the development of the vertebrate head. However, loss-of-function mutations in egfl6 did not cause any craniofacial defects, including the pouches as well as the thymus and facial cartilages whose development is contingent upon appropriate pouch formation. egfl6 was unlikely redundant with egfl7 expressed in a distinct pharyngeal region from that of egfl6 in craniofacial development because reduction of egfl7 with a MO in egfl6 mutants did not affect craniofacial development. In addition, we found that egfl6 carried an endogenous start loss mutation in the wild-type Tübingen strain, implying egfl6 would be a non-functional gene. Taken all together, we suggest that egfl6 expression in the pharyngeal pouches is not required for craniofacial development in zebrafish.Sil JinHyejee NaHaewon JeonJangwon ParkChong Pyo ChoeTaylor & Francis Grouparticleegfl6pharyngeal pouchcraniofacial developmentzebrafishMedicine (General)R5-920Biology (General)QH301-705.5ENAnimal Cells and Systems, Vol 25, Iss 5, Pp 255-263 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	egfl6 pharyngeal pouch craniofacial development zebrafish Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	egfl6 pharyngeal pouch craniofacial development zebrafish Medicine (General) R5-920 Biology (General) QH301-705.5 Sil Jin Hyejee Na Haewon Jeon Jangwon Park Chong Pyo Choe egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development
description	Epidermal growth factor-like domain multiple 6 (Egfl6) is a basement membrane protein and plays an important role in hair follicle morphogenesis, angiogenesis, notochord development in vertebrates. Although egfl6 expression in the developing head was observed in zebrafish, its role for craniofacial development and the determination of the pharyngeal region expressing egfl6, have not been reported yet. Here, we report the expression patterns and function of egfl6 in craniofacial development in zebrafish. egfl6 was expressed sequentially in the developing pharyngeal pouches that are key epithelial structures governing the development of the vertebrate head. However, loss-of-function mutations in egfl6 did not cause any craniofacial defects, including the pouches as well as the thymus and facial cartilages whose development is contingent upon appropriate pouch formation. egfl6 was unlikely redundant with egfl7 expressed in a distinct pharyngeal region from that of egfl6 in craniofacial development because reduction of egfl7 with a MO in egfl6 mutants did not affect craniofacial development. In addition, we found that egfl6 carried an endogenous start loss mutation in the wild-type Tübingen strain, implying egfl6 would be a non-functional gene. Taken all together, we suggest that egfl6 expression in the pharyngeal pouches is not required for craniofacial development in zebrafish.
format	article
author	Sil Jin Hyejee Na Haewon Jeon Jangwon Park Chong Pyo Choe
author_facet	Sil Jin Hyejee Na Haewon Jeon Jangwon Park Chong Pyo Choe
author_sort	Sil Jin
title	egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development
title_short	egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development
title_full	egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development
title_fullStr	egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development
title_full_unstemmed	egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development
title_sort	egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/2be1e7bc76c042deae4f25fc11fc5dae
work_keys_str_mv	AT siljin egfl6expressioninthepharyngealpouchisdispensableforcraniofacialdevelopment AT hyejeena egfl6expressioninthepharyngealpouchisdispensableforcraniofacialdevelopment AT haewonjeon egfl6expressioninthepharyngealpouchisdispensableforcraniofacialdevelopment AT jangwonpark egfl6expressioninthepharyngealpouchisdispensableforcraniofacialdevelopment AT chongpyochoe egfl6expressioninthepharyngealpouchisdispensableforcraniofacialdevelopment
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egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development

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