IL-17/Th17 pathway is activated in acne lesions.

The mechanisms of inflammation in acne are currently subject of intense investigation. This study focused on the activation of adaptive and innate immunity in clinically early visible inflamed acne lesions and was performed in two independent patient populations. Biopsies were collected from lesiona...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Hanna-Leena Kelhälä, Riitta Palatsi, Nanna Fyhrquist, Sari Lehtimäki, Juha P Väyrynen, Matti Kallioinen, Minna E Kubin, Dario Greco, Kaisa Tasanen, Harri Alenius, Beatrice Bertino, Isabelle Carlavan, Bruno Mehul, Sophie Déret, Pascale Reiniche, Philippe Martel, Carine Marty, Ulrike Blume-Peytavi, Johannes J Voegel, Antti Lauerma
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
R
Q
Acceso en línea:https://doaj.org/article/2be799c3d05146d78144b913059b859a
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:2be799c3d05146d78144b913059b859a
record_format dspace
spelling oai:doaj.org-article:2be799c3d05146d78144b913059b859a2021-11-25T06:03:20ZIL-17/Th17 pathway is activated in acne lesions.1932-620310.1371/journal.pone.0105238https://doaj.org/article/2be799c3d05146d78144b913059b859a2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25153527/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The mechanisms of inflammation in acne are currently subject of intense investigation. This study focused on the activation of adaptive and innate immunity in clinically early visible inflamed acne lesions and was performed in two independent patient populations. Biopsies were collected from lesional and non-lesional skin of acne patients. Using Affymetrix Genechips, we observed significant elevation of the signature cytokines of the Th17 lineage in acne lesions compared to non-lesional skin. The increased expression of IL-17 was confirmed at the RNA and also protein level with real-time PCR (RT-PCR) and Luminex technology. Cytokines involved in Th17 lineage differentiation (IL-1β, IL-6, TGF-β, IL23p19) were remarkably induced at the RNA level. In addition, proinflammatory cytokines and chemokines (TNF-α, IL-8, CSF2 and CCL20), Th1 markers (IL12p40, CXCR3, T-bet, IFN-γ), T regulatory cell markers (Foxp3, IL-10, TGF-β) and IL-17 related antimicrobial peptides (S100A7, S100A9, lipocalin, hBD2, hBD3, hCAP18) were induced. Importantly, immunohistochemistry revealed significantly increased numbers of IL-17A positive T cells and CD83 dendritic cells in the acne lesions. In summary our results demonstrate the presence of IL-17A positive T cells and the activation of Th17-related cytokines in acne lesions, indicating that the Th17 pathway is activated and may play a pivotal role in the disease process, possibly offering new targets of therapy.Hanna-Leena KelhäläRiitta PalatsiNanna FyhrquistSari LehtimäkiJuha P VäyrynenMatti KallioinenMinna E KubinDario GrecoKaisa TasanenHarri AleniusBeatrice BertinoIsabelle CarlavanBruno MehulSophie DéretPascale ReinichePhilippe MartelCarine MartyUlrike Blume-PeytaviJohannes J VoegelAntti LauermaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105238 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Hanna-Leena Kelhälä
Riitta Palatsi
Nanna Fyhrquist
Sari Lehtimäki
Juha P Väyrynen
Matti Kallioinen
Minna E Kubin
Dario Greco
Kaisa Tasanen
Harri Alenius
Beatrice Bertino
Isabelle Carlavan
Bruno Mehul
Sophie Déret
Pascale Reiniche
Philippe Martel
Carine Marty
Ulrike Blume-Peytavi
Johannes J Voegel
Antti Lauerma
IL-17/Th17 pathway is activated in acne lesions.
description The mechanisms of inflammation in acne are currently subject of intense investigation. This study focused on the activation of adaptive and innate immunity in clinically early visible inflamed acne lesions and was performed in two independent patient populations. Biopsies were collected from lesional and non-lesional skin of acne patients. Using Affymetrix Genechips, we observed significant elevation of the signature cytokines of the Th17 lineage in acne lesions compared to non-lesional skin. The increased expression of IL-17 was confirmed at the RNA and also protein level with real-time PCR (RT-PCR) and Luminex technology. Cytokines involved in Th17 lineage differentiation (IL-1β, IL-6, TGF-β, IL23p19) were remarkably induced at the RNA level. In addition, proinflammatory cytokines and chemokines (TNF-α, IL-8, CSF2 and CCL20), Th1 markers (IL12p40, CXCR3, T-bet, IFN-γ), T regulatory cell markers (Foxp3, IL-10, TGF-β) and IL-17 related antimicrobial peptides (S100A7, S100A9, lipocalin, hBD2, hBD3, hCAP18) were induced. Importantly, immunohistochemistry revealed significantly increased numbers of IL-17A positive T cells and CD83 dendritic cells in the acne lesions. In summary our results demonstrate the presence of IL-17A positive T cells and the activation of Th17-related cytokines in acne lesions, indicating that the Th17 pathway is activated and may play a pivotal role in the disease process, possibly offering new targets of therapy.
format article
author Hanna-Leena Kelhälä
Riitta Palatsi
Nanna Fyhrquist
Sari Lehtimäki
Juha P Väyrynen
Matti Kallioinen
Minna E Kubin
Dario Greco
Kaisa Tasanen
Harri Alenius
Beatrice Bertino
Isabelle Carlavan
Bruno Mehul
Sophie Déret
Pascale Reiniche
Philippe Martel
Carine Marty
Ulrike Blume-Peytavi
Johannes J Voegel
Antti Lauerma
author_facet Hanna-Leena Kelhälä
Riitta Palatsi
Nanna Fyhrquist
Sari Lehtimäki
Juha P Väyrynen
Matti Kallioinen
Minna E Kubin
Dario Greco
Kaisa Tasanen
Harri Alenius
Beatrice Bertino
Isabelle Carlavan
Bruno Mehul
Sophie Déret
Pascale Reiniche
Philippe Martel
Carine Marty
Ulrike Blume-Peytavi
Johannes J Voegel
Antti Lauerma
author_sort Hanna-Leena Kelhälä
title IL-17/Th17 pathway is activated in acne lesions.
title_short IL-17/Th17 pathway is activated in acne lesions.
title_full IL-17/Th17 pathway is activated in acne lesions.
title_fullStr IL-17/Th17 pathway is activated in acne lesions.
title_full_unstemmed IL-17/Th17 pathway is activated in acne lesions.
title_sort il-17/th17 pathway is activated in acne lesions.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/2be799c3d05146d78144b913059b859a
work_keys_str_mv AT hannaleenakelhala il17th17pathwayisactivatedinacnelesions
AT riittapalatsi il17th17pathwayisactivatedinacnelesions
AT nannafyhrquist il17th17pathwayisactivatedinacnelesions
AT sarilehtimaki il17th17pathwayisactivatedinacnelesions
AT juhapvayrynen il17th17pathwayisactivatedinacnelesions
AT mattikallioinen il17th17pathwayisactivatedinacnelesions
AT minnaekubin il17th17pathwayisactivatedinacnelesions
AT dariogreco il17th17pathwayisactivatedinacnelesions
AT kaisatasanen il17th17pathwayisactivatedinacnelesions
AT harrialenius il17th17pathwayisactivatedinacnelesions
AT beatricebertino il17th17pathwayisactivatedinacnelesions
AT isabellecarlavan il17th17pathwayisactivatedinacnelesions
AT brunomehul il17th17pathwayisactivatedinacnelesions
AT sophiederet il17th17pathwayisactivatedinacnelesions
AT pascalereiniche il17th17pathwayisactivatedinacnelesions
AT philippemartel il17th17pathwayisactivatedinacnelesions
AT carinemarty il17th17pathwayisactivatedinacnelesions
AT ulrikeblumepeytavi il17th17pathwayisactivatedinacnelesions
AT johannesjvoegel il17th17pathwayisactivatedinacnelesions
AT anttilauerma il17th17pathwayisactivatedinacnelesions
_version_ 1718414213241634816