X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning

Abstract X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron microscopy for submicrometer thick specimens, and the molecule-specific imaging capabilites of fluorescence light microscopy. We describe here the...

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Autores principales:	Junjing Deng, David J. Vine, Si Chen, Qiaoling Jin, Youssef S. G. Nashed, Tom Peterka, Stefan Vogt, Chris Jacobsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2bea7a6aee8c45a792653c273a78fc6f
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spelling	oai:doaj.org-article:2bea7a6aee8c45a792653c273a78fc6f2021-12-02T16:06:08ZX-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning10.1038/s41598-017-00569-y2045-2322https://doaj.org/article/2bea7a6aee8c45a792653c273a78fc6f2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00569-yhttps://doaj.org/toc/2045-2322Abstract X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron microscopy for submicrometer thick specimens, and the molecule-specific imaging capabilites of fluorescence light microscopy. We describe here the first use of fast, continuous x-ray scanning of frozen hydrated cells for simultaneous sub-20 nm resolution ptychographic transmission imaging with high contrast, and sub-100 nm resolution deconvolved x-ray fluorescence imaging of diffusible and bound ions at native concentrations, without the need to add specific labels. By working with cells that have been rapidly frozen without the use of chemical fixatives, and imaging them under cryogenic conditions, we are able to obtain images with well preserved structural and chemical composition, and sufficient stability against radiation damage to allow for multiple images to be obtained with no observable change.Junjing DengDavid J. VineSi ChenQiaoling JinYoussef S. G. NashedTom PeterkaStefan VogtChris JacobsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Junjing Deng David J. Vine Si Chen Qiaoling Jin Youssef S. G. Nashed Tom Peterka Stefan Vogt Chris Jacobsen X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
description	Abstract X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron microscopy for submicrometer thick specimens, and the molecule-specific imaging capabilites of fluorescence light microscopy. We describe here the first use of fast, continuous x-ray scanning of frozen hydrated cells for simultaneous sub-20 nm resolution ptychographic transmission imaging with high contrast, and sub-100 nm resolution deconvolved x-ray fluorescence imaging of diffusible and bound ions at native concentrations, without the need to add specific labels. By working with cells that have been rapidly frozen without the use of chemical fixatives, and imaging them under cryogenic conditions, we are able to obtain images with well preserved structural and chemical composition, and sufficient stability against radiation damage to allow for multiple images to be obtained with no observable change.
format	article
author	Junjing Deng David J. Vine Si Chen Qiaoling Jin Youssef S. G. Nashed Tom Peterka Stefan Vogt Chris Jacobsen
author_facet	Junjing Deng David J. Vine Si Chen Qiaoling Jin Youssef S. G. Nashed Tom Peterka Stefan Vogt Chris Jacobsen
author_sort	Junjing Deng
title	X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
title_short	X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
title_full	X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
title_fullStr	X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
title_full_unstemmed	X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
title_sort	x-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2bea7a6aee8c45a792653c273a78fc6f
work_keys_str_mv	AT junjingdeng xrayptychographicandfluorescencemicroscopyoffrozenhydratedcellsusingcontinuousscanning AT davidjvine xrayptychographicandfluorescencemicroscopyoffrozenhydratedcellsusingcontinuousscanning AT sichen xrayptychographicandfluorescencemicroscopyoffrozenhydratedcellsusingcontinuousscanning AT qiaolingjin xrayptychographicandfluorescencemicroscopyoffrozenhydratedcellsusingcontinuousscanning AT youssefsgnashed xrayptychographicandfluorescencemicroscopyoffrozenhydratedcellsusingcontinuousscanning AT tompeterka xrayptychographicandfluorescencemicroscopyoffrozenhydratedcellsusingcontinuousscanning AT stefanvogt xrayptychographicandfluorescencemicroscopyoffrozenhydratedcellsusingcontinuousscanning AT chrisjacobsen xrayptychographicandfluorescencemicroscopyoffrozenhydratedcellsusingcontinuousscanning
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X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning

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