HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney

We previously demonstrated that mast cells expressing HLA-G are associated with regions of hepatitis C virus-induced liver fibrosis. Here, we aimed to determine whether HLA-G expression in mast cells is specific to viral etiology, the liver, or to the general process of fibrosis. We enumerated HLA-G...

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Autores principales:	Nicolas Mouchet, Nicolas Vu, Bruno Turlin, Nathalie Rioux-Leclercq, Stéphane Jouneau, Michel Samson, Laurence Amiot
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	fibrosis HLA-G mast cells follicles B lymphocytes antifibrotic Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2bee05bc1fdd400cb57808d3d2faf44b
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spelling	oai:doaj.org-article:2bee05bc1fdd400cb57808d3d2faf44b2021-11-25T17:57:13ZHLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney10.3390/ijms2222124901422-00671661-6596https://doaj.org/article/2bee05bc1fdd400cb57808d3d2faf44b2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12490https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067We previously demonstrated that mast cells expressing HLA-G are associated with regions of hepatitis C virus-induced liver fibrosis. Here, we aimed to determine whether HLA-G expression in mast cells is specific to viral etiology, the liver, or to the general process of fibrosis. We enumerated HLA-G<sup>+</sup> cells and mast cells by the immunohistochemistry of (i) liver blocks from 41 cases of alcoholic cirrhosis, (ii) 10 of idiopathic pulmonary fibrosis (IPF), and (iii) 10 of renal fibrosis. The nature of the HLA-G<sup>+</sup> cells was specified by multiplex immunofluorescence using software. More than half of all HLA-G<sup>+</sup> cells were mast cells in fibrotic areas of alcoholic cirrhosis and IPF. In the kidneys, subjected to fibrosis, the HLA-G<sup>+</sup> cells were indeed mast cells but could not be counted. Moreover, in certain cases of the liver and lung, we observed a number of cellular nodes, which were secondary or tertiary follicles, in which HLA-G was highly expressed by B lymphocytes. In conclusion, HLA-G<sup>+</sup> mast cells could be observed in the fibrotic regions of all organs studied. Previous studies suggest a protective role for HLA-G<sup>+</sup> mast cells against inflammation and fibrosis. The observed follicles with B lymphocytes that express HLA-G may also reinforce their antifibrotic role.Nicolas MouchetNicolas VuBruno TurlinNathalie Rioux-LeclercqStéphane JouneauMichel SamsonLaurence AmiotMDPI AGarticlefibrosisHLA-Gmast cellsfolliclesB lymphocytesantifibroticBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12490, p 12490 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fibrosis HLA-G mast cells follicles B lymphocytes antifibrotic Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	fibrosis HLA-G mast cells follicles B lymphocytes antifibrotic Biology (General) QH301-705.5 Chemistry QD1-999 Nicolas Mouchet Nicolas Vu Bruno Turlin Nathalie Rioux-Leclercq Stéphane Jouneau Michel Samson Laurence Amiot HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney
description	We previously demonstrated that mast cells expressing HLA-G are associated with regions of hepatitis C virus-induced liver fibrosis. Here, we aimed to determine whether HLA-G expression in mast cells is specific to viral etiology, the liver, or to the general process of fibrosis. We enumerated HLA-G<sup>+</sup> cells and mast cells by the immunohistochemistry of (i) liver blocks from 41 cases of alcoholic cirrhosis, (ii) 10 of idiopathic pulmonary fibrosis (IPF), and (iii) 10 of renal fibrosis. The nature of the HLA-G<sup>+</sup> cells was specified by multiplex immunofluorescence using software. More than half of all HLA-G<sup>+</sup> cells were mast cells in fibrotic areas of alcoholic cirrhosis and IPF. In the kidneys, subjected to fibrosis, the HLA-G<sup>+</sup> cells were indeed mast cells but could not be counted. Moreover, in certain cases of the liver and lung, we observed a number of cellular nodes, which were secondary or tertiary follicles, in which HLA-G was highly expressed by B lymphocytes. In conclusion, HLA-G<sup>+</sup> mast cells could be observed in the fibrotic regions of all organs studied. Previous studies suggest a protective role for HLA-G<sup>+</sup> mast cells against inflammation and fibrosis. The observed follicles with B lymphocytes that express HLA-G may also reinforce their antifibrotic role.
format	article
author	Nicolas Mouchet Nicolas Vu Bruno Turlin Nathalie Rioux-Leclercq Stéphane Jouneau Michel Samson Laurence Amiot
author_facet	Nicolas Mouchet Nicolas Vu Bruno Turlin Nathalie Rioux-Leclercq Stéphane Jouneau Michel Samson Laurence Amiot
author_sort	Nicolas Mouchet
title	HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney
title_short	HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney
title_full	HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney
title_fullStr	HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney
title_full_unstemmed	HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney
title_sort	hla-g is widely expressed by mast cells in regions of organ fibrosis in the liver, lung and kidney
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2bee05bc1fdd400cb57808d3d2faf44b
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_version_	1718411791384444928

HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney

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