Effects of Training Status and Exercise Mode on Global Gene Expression in Skeletal Muscle

The aim of this study was to investigate differences in skeletal muscle gene expression of highly trained endurance and strength athletes in comparison to untrained individuals at rest and in response to either an acute bout of endurance or strength exercise. Endurance (ET, <i>n</i> = 8,...

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Autores principales:	Daniel A. Bizjak, Martina Zügel, Gunnar Treff, Kay Winkert, Achim Jerg, Jens Hudemann, Frank C. Mooren, Karsten Krüger, Andreas Nieß, Jürgen M. Steinacker
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	training status transcriptional regulation endurance exercise strength exercise microarray pathway analysis Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2bf0288f59924ba98941fcfb91d6242c
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spelling	oai:doaj.org-article:2bf0288f59924ba98941fcfb91d6242c2021-11-25T17:58:01ZEffects of Training Status and Exercise Mode on Global Gene Expression in Skeletal Muscle10.3390/ijms2222125781422-00671661-6596https://doaj.org/article/2bf0288f59924ba98941fcfb91d6242c2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12578https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The aim of this study was to investigate differences in skeletal muscle gene expression of highly trained endurance and strength athletes in comparison to untrained individuals at rest and in response to either an acute bout of endurance or strength exercise. Endurance (ET, <i>n</i> = 8, VO<sub>2</sub>max 67 ± 9 mL/kg/min) and strength athletes (ST, <i>n</i> = 8, 5.8 ± 3.0 training years) as well as untrained controls (E-UT and S-UT, each <i>n</i> = 8) performed an acute endurance or strength exercise test. One day before testing (Pre), 30 min (30′Post) and 3 h (180′Post) afterwards, a skeletal muscle biopsy was obtained from the m. vastus lateralis. Skeletal muscle mRNA was isolated and analyzed by Affymetrix-microarray technology. Pathway analyses were performed to evaluate the effects of training status (trained vs. untrained) and exercise mode-specific (ET vs. ST) transcriptional responses. Differences in global skeletal muscle gene expression between trained and untrained were smaller compared to differences in exercise mode. Maximum differences between ET and ST were found between Pre and 180′Post. Pathway analyses showed increased expression of exercise-related genes, such as nuclear transcription factors (NR4A family), metabolism and vascularization (PGC1-α and VEGF-A), and muscle growth/structure (myostatin, IRS1/2 and HIF1-α. The most upregulated genes in response to acute endurance or strength exercise were the NR4A genes (NR4A1, NR4A2, NR4A3). The mode of acute exercise had a significant effect on transcriptional regulation Pre vs. 180′Post. In contrast, the effect of training status on human skeletal muscle gene expression profiles was negligible compared to strength or endurance specialization. The highest variability in gene expression, especially for the NR4A-family, was observed in trained individuals at 180′Post. Assessment of these receptors might be suitable to obtain a deeper understanding of skeletal muscle adaptive processes to develop optimized training strategies.Daniel A. BizjakMartina ZügelGunnar TreffKay WinkertAchim JergJens HudemannFrank C. MoorenKarsten KrügerAndreas NießJürgen M. SteinackerMDPI AGarticletraining statustranscriptional regulationendurance exercisestrength exercisemicroarraypathway analysisBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12578, p 12578 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	training status transcriptional regulation endurance exercise strength exercise microarray pathway analysis Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	training status transcriptional regulation endurance exercise strength exercise microarray pathway analysis Biology (General) QH301-705.5 Chemistry QD1-999 Daniel A. Bizjak Martina Zügel Gunnar Treff Kay Winkert Achim Jerg Jens Hudemann Frank C. Mooren Karsten Krüger Andreas Nieß Jürgen M. Steinacker Effects of Training Status and Exercise Mode on Global Gene Expression in Skeletal Muscle
description	The aim of this study was to investigate differences in skeletal muscle gene expression of highly trained endurance and strength athletes in comparison to untrained individuals at rest and in response to either an acute bout of endurance or strength exercise. Endurance (ET, <i>n</i> = 8, VO<sub>2</sub>max 67 ± 9 mL/kg/min) and strength athletes (ST, <i>n</i> = 8, 5.8 ± 3.0 training years) as well as untrained controls (E-UT and S-UT, each <i>n</i> = 8) performed an acute endurance or strength exercise test. One day before testing (Pre), 30 min (30′Post) and 3 h (180′Post) afterwards, a skeletal muscle biopsy was obtained from the m. vastus lateralis. Skeletal muscle mRNA was isolated and analyzed by Affymetrix-microarray technology. Pathway analyses were performed to evaluate the effects of training status (trained vs. untrained) and exercise mode-specific (ET vs. ST) transcriptional responses. Differences in global skeletal muscle gene expression between trained and untrained were smaller compared to differences in exercise mode. Maximum differences between ET and ST were found between Pre and 180′Post. Pathway analyses showed increased expression of exercise-related genes, such as nuclear transcription factors (NR4A family), metabolism and vascularization (PGC1-α and VEGF-A), and muscle growth/structure (myostatin, IRS1/2 and HIF1-α. The most upregulated genes in response to acute endurance or strength exercise were the NR4A genes (NR4A1, NR4A2, NR4A3). The mode of acute exercise had a significant effect on transcriptional regulation Pre vs. 180′Post. In contrast, the effect of training status on human skeletal muscle gene expression profiles was negligible compared to strength or endurance specialization. The highest variability in gene expression, especially for the NR4A-family, was observed in trained individuals at 180′Post. Assessment of these receptors might be suitable to obtain a deeper understanding of skeletal muscle adaptive processes to develop optimized training strategies.
format	article
author	Daniel A. Bizjak Martina Zügel Gunnar Treff Kay Winkert Achim Jerg Jens Hudemann Frank C. Mooren Karsten Krüger Andreas Nieß Jürgen M. Steinacker
author_facet	Daniel A. Bizjak Martina Zügel Gunnar Treff Kay Winkert Achim Jerg Jens Hudemann Frank C. Mooren Karsten Krüger Andreas Nieß Jürgen M. Steinacker
author_sort	Daniel A. Bizjak
title	Effects of Training Status and Exercise Mode on Global Gene Expression in Skeletal Muscle
title_short	Effects of Training Status and Exercise Mode on Global Gene Expression in Skeletal Muscle
title_full	Effects of Training Status and Exercise Mode on Global Gene Expression in Skeletal Muscle
title_fullStr	Effects of Training Status and Exercise Mode on Global Gene Expression in Skeletal Muscle
title_full_unstemmed	Effects of Training Status and Exercise Mode on Global Gene Expression in Skeletal Muscle
title_sort	effects of training status and exercise mode on global gene expression in skeletal muscle
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2bf0288f59924ba98941fcfb91d6242c
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Effects of Training Status and Exercise Mode on Global Gene Expression in Skeletal Muscle

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