Prevention of Ophthalmia Neonatorum Caused by <italic toggle="yes">Neisseria gonorrhoeae</italic> Using a Fatty Acid-Based Formulation

ABSTRACT Ophthalmia neonatorum, also called neonatal conjunctivitis, acquired during delivery can occur in the first 28 days of life. Commonly caused by the bacterial pathogen Neisseria gonorrhoeae, infection can lead to corneal scarring, perforation of the eye, and blindness. One approach that can...

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Autores principales:	Colin P. Churchward, Raid G. Alany, Ruth S. Kirk, Anthony J. Walker, Lori A. S. Snyder
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	antibiotic resistance eye infection fatty acids gonococcus Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2bf5a61dd3b94d73a3030663b2ce5dec
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spelling	oai:doaj.org-article:2bf5a61dd3b94d73a3030663b2ce5dec2021-11-15T15:51:42ZPrevention of Ophthalmia Neonatorum Caused by <italic toggle="yes">Neisseria gonorrhoeae</italic> Using a Fatty Acid-Based Formulation10.1128/mBio.00534-172150-7511https://doaj.org/article/2bf5a61dd3b94d73a3030663b2ce5dec2017-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00534-17https://doaj.org/toc/2150-7511ABSTRACT Ophthalmia neonatorum, also called neonatal conjunctivitis, acquired during delivery can occur in the first 28 days of life. Commonly caused by the bacterial pathogen Neisseria gonorrhoeae, infection can lead to corneal scarring, perforation of the eye, and blindness. One approach that can be taken to prevent the disease is the use of an ophthalmic prophylaxis, which kills the bacteria on the surface of the eye shortly after birth. Current prophylaxes are based on antibiotic ointments. However, N. gonorrhoeae is resistant to many antibiotics and alternative treatments must be developed before the condition becomes untreatable. This study focused on developing a fatty acid-based prophylaxis. For this, 37 fatty acids or fatty acid derivatives were screened in vitro for fast antigonococcal activity. Seven candidates were identified as bactericidal at 1 mM. These seven were subjected to irritation testing using three separate methods: the bovine corneal opacity and permeability (BCOP) test; the hen’s egg test—chorioallantoic membrane (HET-CAM); and the red blood cell (RBC) lysis assay. The candidates were also tested in artificial tear fluid to determine whether they were effective in this environment. Four of the candidates remained effective. Among these, two lead candidates, monocaprin and myristoleic acid, displayed the best potential as active compounds in the development of a fatty acid-based prophylaxis for prevention of ophthalmia neonatorum.Colin P. ChurchwardRaid G. AlanyRuth S. KirkAnthony J. WalkerLori A. S. SnyderAmerican Society for Microbiologyarticleantibiotic resistanceeye infectionfatty acidsgonococcusMicrobiologyQR1-502ENmBio, Vol 8, Iss 4 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic resistance eye infection fatty acids gonococcus Microbiology QR1-502
spellingShingle	antibiotic resistance eye infection fatty acids gonococcus Microbiology QR1-502 Colin P. Churchward Raid G. Alany Ruth S. Kirk Anthony J. Walker Lori A. S. Snyder Prevention of Ophthalmia Neonatorum Caused by <italic toggle="yes">Neisseria gonorrhoeae</italic> Using a Fatty Acid-Based Formulation
description	ABSTRACT Ophthalmia neonatorum, also called neonatal conjunctivitis, acquired during delivery can occur in the first 28 days of life. Commonly caused by the bacterial pathogen Neisseria gonorrhoeae, infection can lead to corneal scarring, perforation of the eye, and blindness. One approach that can be taken to prevent the disease is the use of an ophthalmic prophylaxis, which kills the bacteria on the surface of the eye shortly after birth. Current prophylaxes are based on antibiotic ointments. However, N. gonorrhoeae is resistant to many antibiotics and alternative treatments must be developed before the condition becomes untreatable. This study focused on developing a fatty acid-based prophylaxis. For this, 37 fatty acids or fatty acid derivatives were screened in vitro for fast antigonococcal activity. Seven candidates were identified as bactericidal at 1 mM. These seven were subjected to irritation testing using three separate methods: the bovine corneal opacity and permeability (BCOP) test; the hen’s egg test—chorioallantoic membrane (HET-CAM); and the red blood cell (RBC) lysis assay. The candidates were also tested in artificial tear fluid to determine whether they were effective in this environment. Four of the candidates remained effective. Among these, two lead candidates, monocaprin and myristoleic acid, displayed the best potential as active compounds in the development of a fatty acid-based prophylaxis for prevention of ophthalmia neonatorum.
format	article
author	Colin P. Churchward Raid G. Alany Ruth S. Kirk Anthony J. Walker Lori A. S. Snyder
author_facet	Colin P. Churchward Raid G. Alany Ruth S. Kirk Anthony J. Walker Lori A. S. Snyder
author_sort	Colin P. Churchward
title	Prevention of Ophthalmia Neonatorum Caused by <italic toggle="yes">Neisseria gonorrhoeae</italic> Using a Fatty Acid-Based Formulation
title_short	Prevention of Ophthalmia Neonatorum Caused by <italic toggle="yes">Neisseria gonorrhoeae</italic> Using a Fatty Acid-Based Formulation
title_full	Prevention of Ophthalmia Neonatorum Caused by <italic toggle="yes">Neisseria gonorrhoeae</italic> Using a Fatty Acid-Based Formulation
title_fullStr	Prevention of Ophthalmia Neonatorum Caused by <italic toggle="yes">Neisseria gonorrhoeae</italic> Using a Fatty Acid-Based Formulation
title_full_unstemmed	Prevention of Ophthalmia Neonatorum Caused by <italic toggle="yes">Neisseria gonorrhoeae</italic> Using a Fatty Acid-Based Formulation
title_sort	prevention of ophthalmia neonatorum caused by <italic toggle="yes">neisseria gonorrhoeae</italic> using a fatty acid-based formulation
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/2bf5a61dd3b94d73a3030663b2ce5dec
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Prevention of Ophthalmia Neonatorum Caused by <italic toggle="yes">Neisseria gonorrhoeae</italic> Using a Fatty Acid-Based Formulation

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