Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense

Abstract Background Bupleurum chinense DC. is a widely used traditional Chinese medicinal plant. Saikosaponins are the major bioactive constituents of B. chinense, but relatively little is known about saikosaponin biosynthesis. In the present study, we performed an integrated analysis of metabolic c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yilian He, Hua Chen, Jun Zhao, Yuxia Yang, Bin Yang, Liang Feng, Yiguan Zhang, Ping Wei, Dabin Hou, Junning Zhao, Ma Yu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Bupleurum chinense DC. Transcriptome Metabolome Saikosaponin biosynthetic pathway Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/2bfa5a3cca514790b2e7059ddca26656
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2bfa5a3cca514790b2e7059ddca26656
record_format	dspace
spelling	oai:doaj.org-article:2bfa5a3cca514790b2e7059ddca266562021-11-21T12:26:35ZTranscriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense10.1186/s12864-021-08144-61471-2164https://doaj.org/article/2bfa5a3cca514790b2e7059ddca266562021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08144-6https://doaj.org/toc/1471-2164Abstract Background Bupleurum chinense DC. is a widely used traditional Chinese medicinal plant. Saikosaponins are the major bioactive constituents of B. chinense, but relatively little is known about saikosaponin biosynthesis. In the present study, we performed an integrated analysis of metabolic composition and the expressed genes involved in saikosaponin biosynthetic pathways among four organs (the root, flower, stem, and leaf) of B. chinense to discover the genes related to the saikosaponin biosynthetic pathway. Results Transcript and metabolite profiles were generated through high-throughput RNA-sequencing (RNA-seq) data analysis and liquid chromatography tandem mass spectrometry, respectively. Evaluation of saikosaponin contents and transcriptional changes showed 152 strong correlations (P < 0.05) over 3 compounds and 77 unigenes. These unigenes belonged to eight gene families: the acetoacetyl CoA transferase (AACT) (6), HMG-CoA synthase (HMGS) (2), HMG-CoA reductase (HMGR) (2), mevalonate diphosphate decarboxylase (MVD) (1), 1-deoxy-D-xylulose-5-phosphate synthase (DXS) (3), farnesyl diphosphate synthase (FPPS) (11), β-amyrin synthase (β-AS) (13) and cytochrome P450 enzymes (P450s) (39) families. Conclusions Our results investigated the diversity of the saikosaponin triterpene biosynthetic pathway in the roots, stems, leaves and flowers of B. chinese by integrated transcriptomic and metabolomic analysis, implying that manipulation of P450s genes such as Bc95697 and Bc35434 might improve saikosaponin biosynthesis. This is a good candidate for the genetic improvement of this important medicinal plant.Yilian HeHua ChenJun ZhaoYuxia YangBin YangLiang FengYiguan ZhangPing WeiDabin HouJunning ZhaoMa YuBMCarticleBupleurum chinense DC. TranscriptomeMetabolomeSaikosaponin biosynthetic pathwayBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bupleurum chinense DC. Transcriptome Metabolome Saikosaponin biosynthetic pathway Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Bupleurum chinense DC. Transcriptome Metabolome Saikosaponin biosynthetic pathway Biotechnology TP248.13-248.65 Genetics QH426-470 Yilian He Hua Chen Jun Zhao Yuxia Yang Bin Yang Liang Feng Yiguan Zhang Ping Wei Dabin Hou Junning Zhao Ma Yu Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense
description	Abstract Background Bupleurum chinense DC. is a widely used traditional Chinese medicinal plant. Saikosaponins are the major bioactive constituents of B. chinense, but relatively little is known about saikosaponin biosynthesis. In the present study, we performed an integrated analysis of metabolic composition and the expressed genes involved in saikosaponin biosynthetic pathways among four organs (the root, flower, stem, and leaf) of B. chinense to discover the genes related to the saikosaponin biosynthetic pathway. Results Transcript and metabolite profiles were generated through high-throughput RNA-sequencing (RNA-seq) data analysis and liquid chromatography tandem mass spectrometry, respectively. Evaluation of saikosaponin contents and transcriptional changes showed 152 strong correlations (P < 0.05) over 3 compounds and 77 unigenes. These unigenes belonged to eight gene families: the acetoacetyl CoA transferase (AACT) (6), HMG-CoA synthase (HMGS) (2), HMG-CoA reductase (HMGR) (2), mevalonate diphosphate decarboxylase (MVD) (1), 1-deoxy-D-xylulose-5-phosphate synthase (DXS) (3), farnesyl diphosphate synthase (FPPS) (11), β-amyrin synthase (β-AS) (13) and cytochrome P450 enzymes (P450s) (39) families. Conclusions Our results investigated the diversity of the saikosaponin triterpene biosynthetic pathway in the roots, stems, leaves and flowers of B. chinese by integrated transcriptomic and metabolomic analysis, implying that manipulation of P450s genes such as Bc95697 and Bc35434 might improve saikosaponin biosynthesis. This is a good candidate for the genetic improvement of this important medicinal plant.
format	article
author	Yilian He Hua Chen Jun Zhao Yuxia Yang Bin Yang Liang Feng Yiguan Zhang Ping Wei Dabin Hou Junning Zhao Ma Yu
author_facet	Yilian He Hua Chen Jun Zhao Yuxia Yang Bin Yang Liang Feng Yiguan Zhang Ping Wei Dabin Hou Junning Zhao Ma Yu
author_sort	Yilian He
title	Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense
title_short	Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense
title_full	Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense
title_fullStr	Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense
title_full_unstemmed	Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense
title_sort	transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in bupleurum chinense
publisher	BMC
publishDate	2021
url	https://doaj.org/article/2bfa5a3cca514790b2e7059ddca26656
work_keys_str_mv	AT yilianhe transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense AT huachen transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense AT junzhao transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense AT yuxiayang transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense AT binyang transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense AT liangfeng transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense AT yiguanzhang transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense AT pingwei transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense AT dabinhou transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense AT junningzhao transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense AT mayu transcriptomeandmetabolomeanalysistorevealmajorgenesofsaikosaponinbiosynthesisinbupleurumchinense
_version_	1718418995556646912

Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense

Ejemplares similares