Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics

Abstract Cell-free DNA (cfDNA) has been implicated as an important biomarker in cancer management. Thus, efficient techniques for cfDNA extraction are necessary for precision medicine. We developed a centrifugation-free cfDNA extraction microfluidic chip capable of extracting cfDNA from plasma sampl...

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Autores principales:	Hoyoon Lee, Chanhee Park, Wonhwi Na, Kyong Hwa Park, Sehyun Shin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/2c032ab2ee9c4444961db1169d4b301b
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spelling	oai:doaj.org-article:2c032ab2ee9c4444961db1169d4b301b2021-12-02T14:18:12ZPrecision cell-free DNA extraction for liquid biopsy by integrated microfluidics10.1038/s41698-019-0107-02397-768Xhttps://doaj.org/article/2c032ab2ee9c4444961db1169d4b301b2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41698-019-0107-0https://doaj.org/toc/2397-768XAbstract Cell-free DNA (cfDNA) has been implicated as an important biomarker in cancer management. Thus, efficient techniques for cfDNA extraction are necessary for precision medicine. We developed a centrifugation-free cfDNA extraction microfluidic chip capable of extracting cfDNA from plasma samples through microfluidic circuits within 15 min under vacuum pressure using an immiscible solvent. The microfluidic chip had excellent performance that was comparable to the most widely used commercial product (QIAamp kit) in terms of extraction efficiency, purity, and quality of DNA samples. The microfluidic chip was validated for the continuous monitoring of HER-2 type breast cancer and was able to successfully detect a point mutation in phosphatidylinositol-4,5-bisphosphate 3-kinase (PIK3CA) during severe liver metastasis. The chip effectively eliminates the repetitive centrifugation processes and dramatically shortened the sample preparation time. The proposed platform could facilitate the development of a sample-to-answer system for use in liquid biopsy of cancers.Hoyoon LeeChanhee ParkWonhwi NaKyong Hwa ParkSehyun ShinNature PortfolioarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Precision Oncology, Vol 4, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Hoyoon Lee Chanhee Park Wonhwi Na Kyong Hwa Park Sehyun Shin Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics
description	Abstract Cell-free DNA (cfDNA) has been implicated as an important biomarker in cancer management. Thus, efficient techniques for cfDNA extraction are necessary for precision medicine. We developed a centrifugation-free cfDNA extraction microfluidic chip capable of extracting cfDNA from plasma samples through microfluidic circuits within 15 min under vacuum pressure using an immiscible solvent. The microfluidic chip had excellent performance that was comparable to the most widely used commercial product (QIAamp kit) in terms of extraction efficiency, purity, and quality of DNA samples. The microfluidic chip was validated for the continuous monitoring of HER-2 type breast cancer and was able to successfully detect a point mutation in phosphatidylinositol-4,5-bisphosphate 3-kinase (PIK3CA) during severe liver metastasis. The chip effectively eliminates the repetitive centrifugation processes and dramatically shortened the sample preparation time. The proposed platform could facilitate the development of a sample-to-answer system for use in liquid biopsy of cancers.
format	article
author	Hoyoon Lee Chanhee Park Wonhwi Na Kyong Hwa Park Sehyun Shin
author_facet	Hoyoon Lee Chanhee Park Wonhwi Na Kyong Hwa Park Sehyun Shin
author_sort	Hoyoon Lee
title	Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics
title_short	Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics
title_full	Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics
title_fullStr	Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics
title_full_unstemmed	Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics
title_sort	precision cell-free dna extraction for liquid biopsy by integrated microfluidics
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2c032ab2ee9c4444961db1169d4b301b
work_keys_str_mv	AT hoyoonlee precisioncellfreednaextractionforliquidbiopsybyintegratedmicrofluidics AT chanheepark precisioncellfreednaextractionforliquidbiopsybyintegratedmicrofluidics AT wonhwina precisioncellfreednaextractionforliquidbiopsybyintegratedmicrofluidics AT kyonghwapark precisioncellfreednaextractionforliquidbiopsybyintegratedmicrofluidics AT sehyunshin precisioncellfreednaextractionforliquidbiopsybyintegratedmicrofluidics
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Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics

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