Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria

Chongwen Wang,1,2,* Bing Gu,3,4,* Qiqi Liu,1,* Yuanfeng Pang,2,5 Rui Xiao,1 Shengqi Wang1–3 1Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases, Beijing Institute of Radiation Medicine, Beijing, People’s Republic of China; 2College of Life Sci...

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Autores principales:	Wang CW, Gu B, Liu QQ, Pang YF, Xiao R, Wang SQ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Surface-enhanced Raman scattering Fe3O4@Ag magnetic nanoparticle Au@Ag nanoaprticles vancomycin-modified rapid bacteria detection Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2c052bcc26cd44c6871dce37a07f01c0
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spelling	oai:doaj.org-article:2c052bcc26cd44c6871dce37a07f01c02021-12-02T04:52:08ZCombined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria1178-2013https://doaj.org/article/2c052bcc26cd44c6871dce37a07f01c02018-02-01T00:00:00Zhttps://www.dovepress.com/combined-use-of-vancomycin-modified-ag-coated-magnetic-nanoparticles-a-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chongwen Wang,1,2,* Bing Gu,3,4,* Qiqi Liu,1,* Yuanfeng Pang,2,5 Rui Xiao,1 Shengqi Wang1–3 1Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases, Beijing Institute of Radiation Medicine, Beijing, People’s Republic of China; 2College of Life Sciences and Bio-Engineering, Beijing University of Technology, Beijing, People’s Republic of China; 3Medical Technology School, Xuzhou Medical University, Xuzhou, People’s Republic of China; 4Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, People’s Republic of China; 5Department of Toxicology, Capital Medical University, Beijing, People’s Republic of China *These authors contributed equally to this work Background: Pathogenic bacteria have always been a significant threat to human health. The detection of pathogens needs to be rapid, accurate, and convenient. Methods: We present a sensitive surface-enhanced Raman scattering (SERS) biosensor based on the combination of vancomycin-modified Ag-coated magnetic nanoparticles (Fe3O4@Ag-Van MNPs) and Au@Ag nanoparticles (NPs) that can effectively capture and discriminate bacterial pathogens from solution. The high-performance Fe3O4@Ag MNPs were modified with vancomycin and used as bacteria capturer for magnetic separation and enrichment. The modified MNPS were found to exhibit strong affinity with a broad range of Gram-positive and Gram-negative bacteria. After separating and rinsing bacteria, Fe3O4@Ag-Van MNPs and Au@Ag NPs were synergistically used to construct a very large number of hot spots on bacteria cells, leading to ultrasensitive SERS detection. Results: The dominant merits of our dual enhanced strategy included high bacterial-capture efficiency (>65%) within a wide pH range (pH 3.0–11.0), a short assay time (<30 min), and a low detection limit (5×102 cells/mL). Moreover, the spiked tests show that this method is still valid in milk and blood samples. Owing to these capabilities, the combined system enabled the sensitive and specific discrimination of different pathogens in complex solution, as verified by its detection of Gram-positive bacterium Escherichia coli, Gram-positive bacterium Staphylococcus aureus, and methicillin-resistant S. aureus. Conclusion: This method has great potential for field applications in food safety, environmental monitoring, and infectious disease diagnosis. Keywords: surface-enhanced Raman scattering, Fe3O4@Ag magnetic nanoparticle, Au@Ag nanoparticles, vancomycin-modified, rapid bacteria detectionWang CWGu BLiu QQPang YFXiao RWang SQDove Medical PressarticleSurface-enhanced Raman scatteringFe3O4@Ag magnetic nanoparticleAu@Ag nanoaprticlesvancomycin-modifiedrapid bacteria detectionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 1159-1178 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Surface-enhanced Raman scattering Fe3O4@Ag magnetic nanoparticle Au@Ag nanoaprticles vancomycin-modified rapid bacteria detection Medicine (General) R5-920
spellingShingle	Surface-enhanced Raman scattering Fe3O4@Ag magnetic nanoparticle Au@Ag nanoaprticles vancomycin-modified rapid bacteria detection Medicine (General) R5-920 Wang CW Gu B Liu QQ Pang YF Xiao R Wang SQ Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria
description	Chongwen Wang,1,2,* Bing Gu,3,4,* Qiqi Liu,1,* Yuanfeng Pang,2,5 Rui Xiao,1 Shengqi Wang1–3 1Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases, Beijing Institute of Radiation Medicine, Beijing, People’s Republic of China; 2College of Life Sciences and Bio-Engineering, Beijing University of Technology, Beijing, People’s Republic of China; 3Medical Technology School, Xuzhou Medical University, Xuzhou, People’s Republic of China; 4Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, People’s Republic of China; 5Department of Toxicology, Capital Medical University, Beijing, People’s Republic of China *These authors contributed equally to this work Background: Pathogenic bacteria have always been a significant threat to human health. The detection of pathogens needs to be rapid, accurate, and convenient. Methods: We present a sensitive surface-enhanced Raman scattering (SERS) biosensor based on the combination of vancomycin-modified Ag-coated magnetic nanoparticles (Fe3O4@Ag-Van MNPs) and Au@Ag nanoparticles (NPs) that can effectively capture and discriminate bacterial pathogens from solution. The high-performance Fe3O4@Ag MNPs were modified with vancomycin and used as bacteria capturer for magnetic separation and enrichment. The modified MNPS were found to exhibit strong affinity with a broad range of Gram-positive and Gram-negative bacteria. After separating and rinsing bacteria, Fe3O4@Ag-Van MNPs and Au@Ag NPs were synergistically used to construct a very large number of hot spots on bacteria cells, leading to ultrasensitive SERS detection. Results: The dominant merits of our dual enhanced strategy included high bacterial-capture efficiency (>65%) within a wide pH range (pH 3.0–11.0), a short assay time (<30 min), and a low detection limit (5×102 cells/mL). Moreover, the spiked tests show that this method is still valid in milk and blood samples. Owing to these capabilities, the combined system enabled the sensitive and specific discrimination of different pathogens in complex solution, as verified by its detection of Gram-positive bacterium Escherichia coli, Gram-positive bacterium Staphylococcus aureus, and methicillin-resistant S. aureus. Conclusion: This method has great potential for field applications in food safety, environmental monitoring, and infectious disease diagnosis. Keywords: surface-enhanced Raman scattering, Fe3O4@Ag magnetic nanoparticle, Au@Ag nanoparticles, vancomycin-modified, rapid bacteria detection
format	article
author	Wang CW Gu B Liu QQ Pang YF Xiao R Wang SQ
author_facet	Wang CW Gu B Liu QQ Pang YF Xiao R Wang SQ
author_sort	Wang CW
title	Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria
title_short	Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria
title_full	Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria
title_fullStr	Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria
title_full_unstemmed	Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria
title_sort	combined use of vancomycin-modified ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced raman scattering detection of bacteria
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/2c052bcc26cd44c6871dce37a07f01c0
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Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria

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