Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>

Biocompatible extraction emerges recently as a means to reduce costs of biotechnology processing of microalgae. In this frame, this study aimed at determining how specific culture conditions and the associated cell morphology impact the biocompatibility and the extraction yield of β-carotene from th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Guillaume Tanguy, Aline Legat, Olivier Gonçalves, Luc Marchal, Benoît Schoefs
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microalgae <i>Dunaliella salina</i> β-carotene in situ extraction milking biocompatibility Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2c0a258ff224436a845faf343239af5c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2c0a258ff224436a845faf343239af5c
record_format	dspace
spelling	oai:doaj.org-article:2c0a258ff224436a845faf343239af5c2021-11-25T18:13:05ZSelection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>10.3390/md191106481660-3397https://doaj.org/article/2c0a258ff224436a845faf343239af5c2021-11-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/648https://doaj.org/toc/1660-3397Biocompatible extraction emerges recently as a means to reduce costs of biotechnology processing of microalgae. In this frame, this study aimed at determining how specific culture conditions and the associated cell morphology impact the biocompatibility and the extraction yield of β-carotene from the green microalga <i>Dunaliella salina</i> using <i>n</i>-decane. The results highlight the relationship between the cell disruption yield and cell volume, the circularity and the relative abundance of naturally permeabilized cells. The disruption rate increased with both the cell volume and circularity. This was particularly obvious for volume and circularity exceeding 1500 µm<sup>3</sup> and 0.7, respectively. The extraction of β-carotene was the most biocompatible with small (600 µm<sup>3</sup>) and circular cells (0.7) stressed in photobioreactor (30% of carotenoids recovery with 15% cell disruption). The naturally permeabilized cells were disrupted first; the remaining cells seems to follow a gradual permeabilization process: reversibility (up to 20 s) then irreversibility and cell disruption. This opens new carotenoid production schemes based on growing robust β-carotene enriched cells to ensure biocompatible extraction.Guillaume TanguyAline LegatOlivier GonçalvesLuc MarchalBenoît SchoefsMDPI AGarticlemicroalgae<i>Dunaliella salina</i>β-carotenein situ extractionmilkingbiocompatibilityBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 648, p 648 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microalgae <i>Dunaliella salina</i> β-carotene in situ extraction milking biocompatibility Biology (General) QH301-705.5
spellingShingle	microalgae <i>Dunaliella salina</i> β-carotene in situ extraction milking biocompatibility Biology (General) QH301-705.5 Guillaume Tanguy Aline Legat Olivier Gonçalves Luc Marchal Benoît Schoefs Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>
description	Biocompatible extraction emerges recently as a means to reduce costs of biotechnology processing of microalgae. In this frame, this study aimed at determining how specific culture conditions and the associated cell morphology impact the biocompatibility and the extraction yield of β-carotene from the green microalga <i>Dunaliella salina</i> using <i>n</i>-decane. The results highlight the relationship between the cell disruption yield and cell volume, the circularity and the relative abundance of naturally permeabilized cells. The disruption rate increased with both the cell volume and circularity. This was particularly obvious for volume and circularity exceeding 1500 µm<sup>3</sup> and 0.7, respectively. The extraction of β-carotene was the most biocompatible with small (600 µm<sup>3</sup>) and circular cells (0.7) stressed in photobioreactor (30% of carotenoids recovery with 15% cell disruption). The naturally permeabilized cells were disrupted first; the remaining cells seems to follow a gradual permeabilization process: reversibility (up to 20 s) then irreversibility and cell disruption. This opens new carotenoid production schemes based on growing robust β-carotene enriched cells to ensure biocompatible extraction.
format	article
author	Guillaume Tanguy Aline Legat Olivier Gonçalves Luc Marchal Benoît Schoefs
author_facet	Guillaume Tanguy Aline Legat Olivier Gonçalves Luc Marchal Benoît Schoefs
author_sort	Guillaume Tanguy
title	Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>
title_short	Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>
title_full	Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>
title_fullStr	Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>
title_full_unstemmed	Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>
title_sort	selection of culture conditions and cell morphology for biocompatible extraction of β-carotene from <i>dunaliella salina</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2c0a258ff224436a845faf343239af5c
work_keys_str_mv	AT guillaumetanguy selectionofcultureconditionsandcellmorphologyforbiocompatibleextractionofbcarotenefromidunaliellasalinai AT alinelegat selectionofcultureconditionsandcellmorphologyforbiocompatibleextractionofbcarotenefromidunaliellasalinai AT oliviergoncalves selectionofcultureconditionsandcellmorphologyforbiocompatibleextractionofbcarotenefromidunaliellasalinai AT lucmarchal selectionofcultureconditionsandcellmorphologyforbiocompatibleextractionofbcarotenefromidunaliellasalinai AT benoitschoefs selectionofcultureconditionsandcellmorphologyforbiocompatibleextractionofbcarotenefromidunaliellasalinai
_version_	1718411426932981760

Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>

Ejemplares similares