Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles

Abstract The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based...

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Autores principales:	Seon-Ju Yeo, Duong Tuan Bao, Ga-Eun Seo, Cuc Thi Bui, Do Thi Hoang Kim, Nguyen Thi Viet Anh, Trinh Thi Thuy Tien, Nguyen Thi Phuong Linh, Hae-Jin Sohn, Chom-Kyu Chong, Ho-Joon Shin, Hyun Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c10ace6d8ce4e6594c60e9731dee104
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spelling	oai:doaj.org-article:2c10ace6d8ce4e6594c60e9731dee1042021-12-02T15:05:33ZImprovement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles10.1038/s41598-017-08328-92045-2322https://doaj.org/article/2c10ace6d8ce4e6594c60e9731dee1042017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08328-9https://doaj.org/toc/2045-2322Abstract The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.Seon-Ju YeoDuong Tuan BaoGa-Eun SeoCuc Thi BuiDo Thi Hoang KimNguyen Thi Viet AnhTrinh Thi Thuy TienNguyen Thi Phuong LinhHae-Jin SohnChom-Kyu ChongHo-Joon ShinHyun ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seon-Ju Yeo Duong Tuan Bao Ga-Eun Seo Cuc Thi Bui Do Thi Hoang Kim Nguyen Thi Viet Anh Trinh Thi Thuy Tien Nguyen Thi Phuong Linh Hae-Jin Sohn Chom-Kyu Chong Ho-Joon Shin Hyun Park Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles
description	Abstract The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.
format	article
author	Seon-Ju Yeo Duong Tuan Bao Ga-Eun Seo Cuc Thi Bui Do Thi Hoang Kim Nguyen Thi Viet Anh Trinh Thi Thuy Tien Nguyen Thi Phuong Linh Hae-Jin Sohn Chom-Kyu Chong Ho-Joon Shin Hyun Park
author_facet	Seon-Ju Yeo Duong Tuan Bao Ga-Eun Seo Cuc Thi Bui Do Thi Hoang Kim Nguyen Thi Viet Anh Trinh Thi Thuy Tien Nguyen Thi Phuong Linh Hae-Jin Sohn Chom-Kyu Chong Ho-Joon Shin Hyun Park
author_sort	Seon-Ju Yeo
title	Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles
title_short	Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles
title_full	Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles
title_fullStr	Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles
title_full_unstemmed	Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles
title_sort	improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza h7 subtype virus using europium nanoparticles
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2c10ace6d8ce4e6594c60e9731dee104
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Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles

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