Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment

Abstract Mycobacterium tuberculosis and M. smegmatis form drug-tolerant biofilms through dedicated genetic programs. In support of a stepwise process regulating biofilm production in mycobacteria, it was shown elsewhere that lsr2 participates in intercellular aggregation, while groEL1 was required f...

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Autores principales:	Mario Alberto Flores-Valdez, Michel de Jesús Aceves-Sánchez, Eliza J. R. Peterson, Nitin Baliga, Jorge Bravo-Madrigal, Miguel Ángel De la Cruz-Villegas, Miguel A. Ares, Sarah Born, Martin Voskuil, Nayeli Areli Pérez-Padilla, Mirna Burciaga-Flores, Tanya Amanda Camacho-Villegas, María Guadalupe Espinoza-Jorge
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c128ef7180346aa9fd5d3b4273f0b57
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spelling	oai:doaj.org-article:2c128ef7180346aa9fd5d3b4273f0b572021-12-02T16:06:39ZTranscriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment10.1038/s41598-020-69152-22045-2322https://doaj.org/article/2c128ef7180346aa9fd5d3b4273f0b572020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-69152-2https://doaj.org/toc/2045-2322Abstract Mycobacterium tuberculosis and M. smegmatis form drug-tolerant biofilms through dedicated genetic programs. In support of a stepwise process regulating biofilm production in mycobacteria, it was shown elsewhere that lsr2 participates in intercellular aggregation, while groEL1 was required for biofilm maturation in M. smegmatis. Here, by means of RNA-Seq, we monitored the early steps of biofilm production in M. bovis BCG, to distinguish intercellular aggregation from attachment to a surface. Genes encoding for the transcriptional regulators dosR and BCG0114 (Rv0081) were significantly regulated and responded differently to intercellular aggregation and surface attachment. Moreover, a M. tuberculosis H37Rv deletion mutant in the Rv3134c-dosS-dosR regulon, formed less biofilm than wild type M. tuberculosis, a phenotype reverted upon reintroduction of this operon into the mutant. Combining RT-qPCR with microbiological assays (colony and surface pellicle morphologies, biofilm quantification, Ziehl–Neelsen staining, growth curve and replication of planktonic cells), we found that BCG0642c affected biofilm production and replication of planktonic BCG, whereas ethR affected only phenotypes linked to planktonic cells despite its downregulation at the intercellular aggregation step. Our results provide evidence for a stage-dependent expression of genes that contribute to biofilm production in slow-growing mycobacteria.Mario Alberto Flores-ValdezMichel de Jesús Aceves-SánchezEliza J. R. PetersonNitin BaligaJorge Bravo-MadrigalMiguel Ángel De la Cruz-VillegasMiguel A. AresSarah BornMartin VoskuilNayeli Areli Pérez-PadillaMirna Burciaga-FloresTanya Amanda Camacho-VillegasMaría Guadalupe Espinoza-JorgeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mario Alberto Flores-Valdez Michel de Jesús Aceves-Sánchez Eliza J. R. Peterson Nitin Baliga Jorge Bravo-Madrigal Miguel Ángel De la Cruz-Villegas Miguel A. Ares Sarah Born Martin Voskuil Nayeli Areli Pérez-Padilla Mirna Burciaga-Flores Tanya Amanda Camacho-Villegas María Guadalupe Espinoza-Jorge Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment
description	Abstract Mycobacterium tuberculosis and M. smegmatis form drug-tolerant biofilms through dedicated genetic programs. In support of a stepwise process regulating biofilm production in mycobacteria, it was shown elsewhere that lsr2 participates in intercellular aggregation, while groEL1 was required for biofilm maturation in M. smegmatis. Here, by means of RNA-Seq, we monitored the early steps of biofilm production in M. bovis BCG, to distinguish intercellular aggregation from attachment to a surface. Genes encoding for the transcriptional regulators dosR and BCG0114 (Rv0081) were significantly regulated and responded differently to intercellular aggregation and surface attachment. Moreover, a M. tuberculosis H37Rv deletion mutant in the Rv3134c-dosS-dosR regulon, formed less biofilm than wild type M. tuberculosis, a phenotype reverted upon reintroduction of this operon into the mutant. Combining RT-qPCR with microbiological assays (colony and surface pellicle morphologies, biofilm quantification, Ziehl–Neelsen staining, growth curve and replication of planktonic cells), we found that BCG0642c affected biofilm production and replication of planktonic BCG, whereas ethR affected only phenotypes linked to planktonic cells despite its downregulation at the intercellular aggregation step. Our results provide evidence for a stage-dependent expression of genes that contribute to biofilm production in slow-growing mycobacteria.
format	article
author	Mario Alberto Flores-Valdez Michel de Jesús Aceves-Sánchez Eliza J. R. Peterson Nitin Baliga Jorge Bravo-Madrigal Miguel Ángel De la Cruz-Villegas Miguel A. Ares Sarah Born Martin Voskuil Nayeli Areli Pérez-Padilla Mirna Burciaga-Flores Tanya Amanda Camacho-Villegas María Guadalupe Espinoza-Jorge
author_facet	Mario Alberto Flores-Valdez Michel de Jesús Aceves-Sánchez Eliza J. R. Peterson Nitin Baliga Jorge Bravo-Madrigal Miguel Ángel De la Cruz-Villegas Miguel A. Ares Sarah Born Martin Voskuil Nayeli Areli Pérez-Padilla Mirna Burciaga-Flores Tanya Amanda Camacho-Villegas María Guadalupe Espinoza-Jorge
author_sort	Mario Alberto Flores-Valdez
title	Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment
title_short	Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment
title_full	Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment
title_fullStr	Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment
title_full_unstemmed	Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment
title_sort	transcriptional portrait of m. bovis bcg during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2c128ef7180346aa9fd5d3b4273f0b57
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Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment

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