The reciprocal interplay between TNFα and the circadian clock impacts on cell proliferation and migration in Hodgkin lymphoma cells

Abstract A bidirectional interaction between the circadian network and effector mechanisms of immunity brings on a proper working of both systems. In the present study, we used Hodgkin lymphoma (HL) as an experimental model for a type of cancer involving cells of the immune system. We identified thi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mónica Abreu, Alireza Basti, Nikolai Genov, Gianluigi Mazzoccoli, Angela Relógio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c1c7ad1f07245c49786e246d325ae18
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2c1c7ad1f07245c49786e246d325ae18
record_format	dspace
spelling	oai:doaj.org-article:2c1c7ad1f07245c49786e246d325ae182021-12-02T11:40:46ZThe reciprocal interplay between TNFα and the circadian clock impacts on cell proliferation and migration in Hodgkin lymphoma cells10.1038/s41598-018-29847-z2045-2322https://doaj.org/article/2c1c7ad1f07245c49786e246d325ae182018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29847-zhttps://doaj.org/toc/2045-2322Abstract A bidirectional interaction between the circadian network and effector mechanisms of immunity brings on a proper working of both systems. In the present study, we used Hodgkin lymphoma (HL) as an experimental model for a type of cancer involving cells of the immune system. We identified this cancer type among haematological malignancies has having a strong differential expression of core-clock elements. Taking advantage of bioinformatics analyses and experimental procedures carried out in III- and IV-stage HL cells, and lymphoblastoid B cells, we explored this interplay and bear out diverse interacting partners of both systems. In particular, we assembled a wide-ranging network of clock-immune-related genes and pinpointed TNF as a crucial intermediary player. A robust circadian clock hallmarked III-stage lymphoma cells, differently from IV-stage HL cells, which do not harbour a properly functioning clockwork. TNF and circadian gene modulation impacted on clock genes expression and triggered phenotypic changes in lymphoma cells, suggesting a crucial involvement of core-clock elements and TNF in the physiopathological mechanisms hastening malignancy. Our results move forward our understanding of the putative role of the core-clock and TNF in the pathobiology of Hodgkin lymphoma, and highlight their influence in cellular proliferation and migration in lymphatic cancers.Mónica AbreuAlireza BastiNikolai GenovGianluigi MazzoccoliAngela RelógioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mónica Abreu Alireza Basti Nikolai Genov Gianluigi Mazzoccoli Angela Relógio The reciprocal interplay between TNFα and the circadian clock impacts on cell proliferation and migration in Hodgkin lymphoma cells
description	Abstract A bidirectional interaction between the circadian network and effector mechanisms of immunity brings on a proper working of both systems. In the present study, we used Hodgkin lymphoma (HL) as an experimental model for a type of cancer involving cells of the immune system. We identified this cancer type among haematological malignancies has having a strong differential expression of core-clock elements. Taking advantage of bioinformatics analyses and experimental procedures carried out in III- and IV-stage HL cells, and lymphoblastoid B cells, we explored this interplay and bear out diverse interacting partners of both systems. In particular, we assembled a wide-ranging network of clock-immune-related genes and pinpointed TNF as a crucial intermediary player. A robust circadian clock hallmarked III-stage lymphoma cells, differently from IV-stage HL cells, which do not harbour a properly functioning clockwork. TNF and circadian gene modulation impacted on clock genes expression and triggered phenotypic changes in lymphoma cells, suggesting a crucial involvement of core-clock elements and TNF in the physiopathological mechanisms hastening malignancy. Our results move forward our understanding of the putative role of the core-clock and TNF in the pathobiology of Hodgkin lymphoma, and highlight their influence in cellular proliferation and migration in lymphatic cancers.
format	article
author	Mónica Abreu Alireza Basti Nikolai Genov Gianluigi Mazzoccoli Angela Relógio
author_facet	Mónica Abreu Alireza Basti Nikolai Genov Gianluigi Mazzoccoli Angela Relógio
author_sort	Mónica Abreu
title	The reciprocal interplay between TNFα and the circadian clock impacts on cell proliferation and migration in Hodgkin lymphoma cells
title_short	The reciprocal interplay between TNFα and the circadian clock impacts on cell proliferation and migration in Hodgkin lymphoma cells
title_full	The reciprocal interplay between TNFα and the circadian clock impacts on cell proliferation and migration in Hodgkin lymphoma cells
title_fullStr	The reciprocal interplay between TNFα and the circadian clock impacts on cell proliferation and migration in Hodgkin lymphoma cells
title_full_unstemmed	The reciprocal interplay between TNFα and the circadian clock impacts on cell proliferation and migration in Hodgkin lymphoma cells
title_sort	reciprocal interplay between tnfα and the circadian clock impacts on cell proliferation and migration in hodgkin lymphoma cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/2c1c7ad1f07245c49786e246d325ae18
work_keys_str_mv	AT monicaabreu thereciprocalinterplaybetweentnfaandthecircadianclockimpactsoncellproliferationandmigrationinhodgkinlymphomacells AT alirezabasti thereciprocalinterplaybetweentnfaandthecircadianclockimpactsoncellproliferationandmigrationinhodgkinlymphomacells AT nikolaigenov thereciprocalinterplaybetweentnfaandthecircadianclockimpactsoncellproliferationandmigrationinhodgkinlymphomacells AT gianluigimazzoccoli thereciprocalinterplaybetweentnfaandthecircadianclockimpactsoncellproliferationandmigrationinhodgkinlymphomacells AT angelarelogio thereciprocalinterplaybetweentnfaandthecircadianclockimpactsoncellproliferationandmigrationinhodgkinlymphomacells AT monicaabreu reciprocalinterplaybetweentnfaandthecircadianclockimpactsoncellproliferationandmigrationinhodgkinlymphomacells AT alirezabasti reciprocalinterplaybetweentnfaandthecircadianclockimpactsoncellproliferationandmigrationinhodgkinlymphomacells AT nikolaigenov reciprocalinterplaybetweentnfaandthecircadianclockimpactsoncellproliferationandmigrationinhodgkinlymphomacells AT gianluigimazzoccoli reciprocalinterplaybetweentnfaandthecircadianclockimpactsoncellproliferationandmigrationinhodgkinlymphomacells AT angelarelogio reciprocalinterplaybetweentnfaandthecircadianclockimpactsoncellproliferationandmigrationinhodgkinlymphomacells
_version_	1718395509339586560

The reciprocal interplay between TNFα and the circadian clock impacts on cell proliferation and migration in Hodgkin lymphoma cells

Ejemplares similares