Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome

Abstract Obesity during pregnancy leads to adverse health outcomes in offspring. However, the initial effects of maternal obesity (MO) on embryonic organogenesis have yet to be thoroughly examined. Using unbiased single‐cell transcriptomic analyses (scRNA‐seq), the effects of MO on the myogenic proc...

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Autores principales:	Liang Zhao, Nathan C. Law, Noe A. Gomez, Junseok Son, Yao Gao, Xiangdong Liu, Jeanene M. de Avila, Mei‐Jun Zhu, Min Du
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	bone morphogenetic proteins signaling embryonic myogenesis maternal obesity single cell RNA sequencing Science Q
Acceso en línea:	https://doaj.org/article/2c1d6c3b39ba4c35911b0956821452ca
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spelling	oai:doaj.org-article:2c1d6c3b39ba4c35911b0956821452ca2021-11-17T08:40:31ZObesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome2198-384410.1002/advs.202102157https://doaj.org/article/2c1d6c3b39ba4c35911b0956821452ca2021-11-01T00:00:00Zhttps://doi.org/10.1002/advs.202102157https://doaj.org/toc/2198-3844Abstract Obesity during pregnancy leads to adverse health outcomes in offspring. However, the initial effects of maternal obesity (MO) on embryonic organogenesis have yet to be thoroughly examined. Using unbiased single‐cell transcriptomic analyses (scRNA‐seq), the effects of MO on the myogenic process is investigated in embryonic day 9.5 (E9.5) mouse embryos. The results suggest that MO induces systematic hypoxia, which is correlated with enhanced BMP signaling and impairs skeletal muscle differentiation within the dermomyotome (DM). The Notch‐signaling effectors, HES1 and HEY1, which also act down‐stream of BMP signaling, suppress myogenic differentiation through transcriptionally repressing the important myogenic regulator MEF2C. Moreover, the major hypoxia effector, HIF1A, enhances expression of HES1 and HEY1 and blocks myogenic differentiation in vitro. In summary, this data demonstrate that MO induces hypoxia and impairs myogenic differentiation by up‐regulating BMP signaling within the DM, which may account for the disruptions of skeletal muscle development and function in progeny.Liang ZhaoNathan C. LawNoe A. GomezJunseok SonYao GaoXiangdong LiuJeanene M. de AvilaMei‐Jun ZhuMin DuWileyarticlebone morphogenetic proteins signalingembryonic myogenesismaternal obesitysingle cell RNA sequencingScienceQENAdvanced Science, Vol 8, Iss 22, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bone morphogenetic proteins signaling embryonic myogenesis maternal obesity single cell RNA sequencing Science Q
spellingShingle	bone morphogenetic proteins signaling embryonic myogenesis maternal obesity single cell RNA sequencing Science Q Liang Zhao Nathan C. Law Noe A. Gomez Junseok Son Yao Gao Xiangdong Liu Jeanene M. de Avila Mei‐Jun Zhu Min Du Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome
description	Abstract Obesity during pregnancy leads to adverse health outcomes in offspring. However, the initial effects of maternal obesity (MO) on embryonic organogenesis have yet to be thoroughly examined. Using unbiased single‐cell transcriptomic analyses (scRNA‐seq), the effects of MO on the myogenic process is investigated in embryonic day 9.5 (E9.5) mouse embryos. The results suggest that MO induces systematic hypoxia, which is correlated with enhanced BMP signaling and impairs skeletal muscle differentiation within the dermomyotome (DM). The Notch‐signaling effectors, HES1 and HEY1, which also act down‐stream of BMP signaling, suppress myogenic differentiation through transcriptionally repressing the important myogenic regulator MEF2C. Moreover, the major hypoxia effector, HIF1A, enhances expression of HES1 and HEY1 and blocks myogenic differentiation in vitro. In summary, this data demonstrate that MO induces hypoxia and impairs myogenic differentiation by up‐regulating BMP signaling within the DM, which may account for the disruptions of skeletal muscle development and function in progeny.
format	article
author	Liang Zhao Nathan C. Law Noe A. Gomez Junseok Son Yao Gao Xiangdong Liu Jeanene M. de Avila Mei‐Jun Zhu Min Du
author_facet	Liang Zhao Nathan C. Law Noe A. Gomez Junseok Son Yao Gao Xiangdong Liu Jeanene M. de Avila Mei‐Jun Zhu Min Du
author_sort	Liang Zhao
title	Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome
title_short	Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome
title_full	Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome
title_fullStr	Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome
title_full_unstemmed	Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome
title_sort	obesity impairs embryonic myogenesis by enhancing bmp signaling within the dermomyotome
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/2c1d6c3b39ba4c35911b0956821452ca
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Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome

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