Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth

Abstract Exosomes are extracellular vesicles secreted by multiple cell types into the extracellular space. They contain cell-state specific cargos which often reflects the (patho)physiological condition of the cells/organism. Milk contains high amounts of exosomes and it is unclear whether their car...

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Autores principales:	Monisha Samuel, David Chisanga, Michael Liem, Shivakumar Keerthikumar, Sushma Anand, Ching-Seng Ang, Christopher G. Adda, Ellen Versteegen, Markandeya Jois, Suresh Mathivanan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c2060f8aedc498487c01047cdddc358
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spelling	oai:doaj.org-article:2c2060f8aedc498487c01047cdddc3582021-12-02T11:40:14ZBovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth10.1038/s41598-017-06288-82045-2322https://doaj.org/article/2c2060f8aedc498487c01047cdddc3582017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06288-8https://doaj.org/toc/2045-2322Abstract Exosomes are extracellular vesicles secreted by multiple cell types into the extracellular space. They contain cell-state specific cargos which often reflects the (patho)physiological condition of the cells/organism. Milk contains high amounts of exosomes and it is unclear whether their cargo is altered based on the lactation stage of the organism. Here, we isolated exosomes from bovine milk that were obtained at various stages of lactation and examined the content by quantitative proteomics. Exosomes were isolated by OptiPrep density gradient centrifugation from milk obtained from cow after 24, 48 and 72 h post calving. As control, exosomes were also isolated from cows during mid-lactation period which has been referred to as mature milk (MM). Biochemical and biophysical characterization of exosomes revealed the high abundance of exosomes in colostrum and MM samples. Quantitative proteomics analysis highlighted the change in the proteomic cargo of exosomes based on the lactation state of the cow. Functional enrichment analysis revealed that exosomes from colostrum are significantly enriched with proteins that can potentially regulate the immune response and growth. This study highlights the importance of exosomes in colostrum and hence opens up new avenues to exploit these vesicles in the regulation of the immune response and growth.Monisha SamuelDavid ChisangaMichael LiemShivakumar KeerthikumarSushma AnandChing-Seng AngChristopher G. AddaEllen VersteegenMarkandeya JoisSuresh MathivananNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Monisha Samuel David Chisanga Michael Liem Shivakumar Keerthikumar Sushma Anand Ching-Seng Ang Christopher G. Adda Ellen Versteegen Markandeya Jois Suresh Mathivanan Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth
description	Abstract Exosomes are extracellular vesicles secreted by multiple cell types into the extracellular space. They contain cell-state specific cargos which often reflects the (patho)physiological condition of the cells/organism. Milk contains high amounts of exosomes and it is unclear whether their cargo is altered based on the lactation stage of the organism. Here, we isolated exosomes from bovine milk that were obtained at various stages of lactation and examined the content by quantitative proteomics. Exosomes were isolated by OptiPrep density gradient centrifugation from milk obtained from cow after 24, 48 and 72 h post calving. As control, exosomes were also isolated from cows during mid-lactation period which has been referred to as mature milk (MM). Biochemical and biophysical characterization of exosomes revealed the high abundance of exosomes in colostrum and MM samples. Quantitative proteomics analysis highlighted the change in the proteomic cargo of exosomes based on the lactation state of the cow. Functional enrichment analysis revealed that exosomes from colostrum are significantly enriched with proteins that can potentially regulate the immune response and growth. This study highlights the importance of exosomes in colostrum and hence opens up new avenues to exploit these vesicles in the regulation of the immune response and growth.
format	article
author	Monisha Samuel David Chisanga Michael Liem Shivakumar Keerthikumar Sushma Anand Ching-Seng Ang Christopher G. Adda Ellen Versteegen Markandeya Jois Suresh Mathivanan
author_facet	Monisha Samuel David Chisanga Michael Liem Shivakumar Keerthikumar Sushma Anand Ching-Seng Ang Christopher G. Adda Ellen Versteegen Markandeya Jois Suresh Mathivanan
author_sort	Monisha Samuel
title	Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth
title_short	Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth
title_full	Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth
title_fullStr	Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth
title_full_unstemmed	Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth
title_sort	bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2c2060f8aedc498487c01047cdddc358
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_version_	1718395632087990272

Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth

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