Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR

Abstract Modern techniques for quantifying blood group antibody-antigen interactions are very limited, especially for weaker interactions which result from low antigen expression and/or partial expression of the antigen structure. Surface plasmon resonance (SPR) detection is often used to monitor an...

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Autores principales:	Whui Lyn Then, Marie-Isabel Aguilar, Gil Garnier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c30a155f26c4d21a3f41b6a453fdbf5
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spelling	oai:doaj.org-article:2c30a155f26c4d21a3f41b6a453fdbf52021-12-02T15:05:05ZQuantitative Detection of Weak D Antigen Variants in Blood Typing using SPR10.1038/s41598-017-01817-x2045-2322https://doaj.org/article/2c30a155f26c4d21a3f41b6a453fdbf52017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01817-xhttps://doaj.org/toc/2045-2322Abstract Modern techniques for quantifying blood group antibody-antigen interactions are very limited, especially for weaker interactions which result from low antigen expression and/or partial expression of the antigen structure. Surface plasmon resonance (SPR) detection is often used to monitor and quantify bio-interactions. Previously, a regenerable, multi-fucntional platform for quantitative RBC phenotyping of normal antigen expression using SPR detection was reported. However, detection of weaker variants were not explored. Here, this sensitivity study used anti-human IgG antibodies immobilized to a gold sensor surface to two clinically important types of weaker D variants using SPR; weak D and partial D. Positive pre-sensitised cells bind to the anti-human IgG monolayer, and the response unit (RU) is reported (>100 RU). Unbound negative cells are directly eluted (<100 RU). Weak D cells were detected between a range of 180–580 RU, due to a lower expression of antigens. Partial D cells, category D VI, were also positively identified (352–1147 RU), similar to that of normal D antigens. The detection of two classes of weaker D variants was achieved for the first time using this fully regenerable SPR platform, opening up a new avenue to replace the current subjective and arbitrary methods for quantifying blood group antibody-antigen interactions.Whui Lyn ThenMarie-Isabel AguilarGil GarnierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Whui Lyn Then Marie-Isabel Aguilar Gil Garnier Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR
description	Abstract Modern techniques for quantifying blood group antibody-antigen interactions are very limited, especially for weaker interactions which result from low antigen expression and/or partial expression of the antigen structure. Surface plasmon resonance (SPR) detection is often used to monitor and quantify bio-interactions. Previously, a regenerable, multi-fucntional platform for quantitative RBC phenotyping of normal antigen expression using SPR detection was reported. However, detection of weaker variants were not explored. Here, this sensitivity study used anti-human IgG antibodies immobilized to a gold sensor surface to two clinically important types of weaker D variants using SPR; weak D and partial D. Positive pre-sensitised cells bind to the anti-human IgG monolayer, and the response unit (RU) is reported (>100 RU). Unbound negative cells are directly eluted (<100 RU). Weak D cells were detected between a range of 180–580 RU, due to a lower expression of antigens. Partial D cells, category D VI, were also positively identified (352–1147 RU), similar to that of normal D antigens. The detection of two classes of weaker D variants was achieved for the first time using this fully regenerable SPR platform, opening up a new avenue to replace the current subjective and arbitrary methods for quantifying blood group antibody-antigen interactions.
format	article
author	Whui Lyn Then Marie-Isabel Aguilar Gil Garnier
author_facet	Whui Lyn Then Marie-Isabel Aguilar Gil Garnier
author_sort	Whui Lyn Then
title	Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR
title_short	Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR
title_full	Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR
title_fullStr	Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR
title_full_unstemmed	Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR
title_sort	quantitative detection of weak d antigen variants in blood typing using spr
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2c30a155f26c4d21a3f41b6a453fdbf5
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_version_	1718388944643555328

Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR

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